We narrowed to 13,002 results for: LIC

1. pBridge-tyrosinase.σ1A

   Plasmid

   #197381

   Purpose

   Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Inserts

   tyrosinase cytosolic tail
   AP-1 σ1A
   

   Use

   Tags

   GAL4-DNA binding domain fragment, HA tag, and SV4…

   Expression

   Yeast

   Mutation

   Contains an extra 42 nucleotides encoding 14 resi…

   Promoter

   ADH1 and MET25

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDest-1.7kbfabp6-eGFP-pA-CrymCherry

   Plasmid

   #159088

   Purpose

   LR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA inserts

   Depositor

   John Rawls

   Article

   Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

   Inserts

   1.7kb fabp6 promoter (fabp6 Zebrafish)
   eGFP
   polyA
   

   Use

   Fluorescent reporter

   Tags

   Expression

   Mutation

   Promoter

   1.7kb fabp6

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB-6HIS-TEV-mEGFP,IRES-NGFR

   Plasmid

   #164521

   Purpose

   All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal His-TEV-GFP tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

   Depositor

   Mitchell Guttman

   Article

   Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223_SLIRP_WT

   Plasmid

   #82288

   Purpose

   Gateway Donor vector containing SLIRP, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   SLIRP (SLIRP Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. miR5 Mm ATP13A2

   Plasmid

   #171822

   Purpose

   transfer plasmid for lentiviral vector production with miR for Mm ATP13A2

   Depositor

   Veerle Baekelandt

   Article

   van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

   Insert

   ATP13A2 (Atp13a2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. miR3 Mm ATP13A2

   Plasmid

   #171771

   Purpose

   transfer plasmid for lentiviral vector production with miR for Mm ATP13A2

   Depositor

   Veerle Baekelandt

   Article

   van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

   Insert

   ATP13A2 (Atp13a2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNIC-ZB-SPRTN-E112A-FL

   Plasmid

   #110218

   Purpose

   Expression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus.

   Depositor

   Kristijan Ramadan

   Article

   Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

   Insert

   SPRTN (SPRTN Human)
   

   Use

   Tags

   His6 and ZB

   Expression

   Bacterial

   Mutation

   E112A, P296L (see depositor comments below)

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA4-His-Max-A-PQBP1-Y65C

   Plasmid

   #25803

   Depositor

   Marius Sudol

   Article

   Tapia et al J Biol Chem. 2010 Jun 18;285(25):19391-4

   Insert

   PQBP1 with Y65C mutation (PQBP1 Human)
   

   Use

   Tags

   His and Xpress

   Expression

   Mammalian

   Mutation

   Tyrosine 65 within the WW domain of PQBP1 is chan…

   Promoter

   Available since

   July 21, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pDONR223_VEGFC_WT

   Plasmid

   #82282

   Purpose

   Gateway Donor vector containing VEGFC, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   VEGFC (VEGFC Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   K414Q

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-HA-Nr4a1-AA

    Plasmid

    #160942

    Purpose

    Generation of retrovirus for the overexpression of Nr4a1 mutant (287C 288EAA)

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Nr4a1 (Nr4a1 Mouse)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Change Cys 287 Glu 288 into Ala Ala

    Promoter

    MSCV-LTR

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_SGK3_WT

    Plasmid

    #82251

    Purpose

    Gateway Donor vector containing SGK3 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SGK3 (SGK3 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223_STAT6_p.N417Y

    Plasmid

    #81591

    Purpose

    Gateway Donor vector containing STAT6 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    STAT6 (STAT6 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    N417Y

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPR-Cas9/CD4-TK2

    Plasmid

    #104397

    Purpose

    The designed sgRNA cloned into this plasmid directs the specific DNA cleavage exerted by Cas9 nuclease in a region of exon 5 of the human TK2 gene. The plasmid also includes the Cas9 nuclease and CD4.

    Depositor

    Jeronimo Blanco

    Article

    Meca-Cortes et al Mol Ther Nucleic Acids. 2017 Sep 15;8:39

    Insert

    TK2 (TK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUSEss-CHIg-mM-Asn209Ala_M18

    Plasmid

    #91733

    Purpose

    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ala (EU numbering).

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

    Insert

    immunoglobulin heavy constant mu (Ighm Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Asn209 changed to Ala (EU numbering)

    Promoter

    hEF1-HTLV prom

    Available since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5-Myc-PSK2 (1-416)

    Plasmid

    #197123

    Purpose

    Expression of Myc-tagged PSK2 (aa1-416) / TAOK1 (aa1-416) in mammalian cells

    Depositor

    Jonathan Morris

    Article

    Tavares et al J Biol Chem. 2013 May 24;288(21):15418-2

    Insert

    TAOK1 (aa1-416) (TAOK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deleted C-terminus

    Promoter

    SV40

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223_SAMHD1_p.D218H

    Plasmid

    #81513

    Purpose

    Gateway Donor vector containing SAMHD1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SAMHD1 (SAMHD1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    D218H; G404E; A546V

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223_VHL_p.S111N

    Plasmid

    #81675

    Purpose

    Gateway Donor vector containing VHL, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    VHL (VHL Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    S111N

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP C1-Eps8 ∆cap/∆bund

    Plasmid

    #74891

    Purpose

    mammalian expression of the capping and bundling activity mutant Eps8 fused to GFP

    Depositor

    Giorgio Scita

    Article

    Hertzog et al PLoS Biol. 2010 Jun 1;8(6):e1000387. doi

    Insert

    Eps8 (Eps8 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    V689D, L693D, L757A, K759A

    Promoter

    Available since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pDONR223_RIMS2_WT

    Plasmid

    #81803

    Purpose

    Gateway Donor vector containing RIMS2 ,part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RIMS2 (RIMS2 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    S86F; Q295R

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGS-FCER1G-1

    Plasmid

    #109192

    Purpose

    Encodes human full-length FCER1G to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    Human full-length FCER1G, codon-optimized (FCER1G Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    p10

    Available since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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