We narrowed to 9,352 results for: ef1
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Plasmid#131255PurposeMammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein)DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
TagsMycExpressionMammalianMutationA mutant form of our pcDNA3.1-N-Myc_535-888 USP7 …Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_DW164AA + DED/AAA USP7
Plasmid#131256PurposeMammalian expression of N-terminally Myc-tagged USP7, with mutations in the TRAF-like domain (DW164AA) and UBL2 (DE758AA_D764A)DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
TagsMycExpressionMammalianMutationContains point mutations that convert USP7 aspart…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-CuO-V5-CASC3 siRes 110-480 188/218-mut
Plasmid#158544PurposeFor PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 110-480DepositorInsertCASC3; BTZ; MLN51 (CASC3 Human)
TagsV5ExpressionMammalianMutationAmino acids 110-480, C-terminal deletion, F188D, …PromoterCMVAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVE-FH-Dnd1-IN
Plasmid#70072Purpose2nd gen lentiviral vector. Cre-removable and tet/dox-controllable expression of FLAG-HA-tagged murine Dnd1 in mammalian cells.DepositorInsertDnd1 (Dnd1 Mouse)
UseCre/Lox and LentiviralTagsFLAG and HAExpressionMammalianPromoterEF1alphaAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-chimeric-IgM-mouse/human_M18
Plasmid#91738PurposeExpression plasmid coding for modified heavy chain of mouse IgM antibody. Residues 203-239 (EU numbering) were exchanged to human homologue sequence.DepositorInsertimmunoglobulin heavy constant mu (Ighm Mouse)
ExpressionMammalianMutationResidues 203-239 (EU numbering) were exchanged to…PromoterhEF1-HTLV promAvailable SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mM-Asp212Ser_M18
Plasmid#91739PurposeExpression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asp212Ser (EU numbering) - N-glycosylated Asn209DepositorInsertimmunoglobulin heavy constant mu (Ighm Mouse)
ExpressionMammalianMutationAsp212 changed to Ser (EU numbering)PromoterhEF1-HTLV promAvailable SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAB2076 pAAV REPAIR.t1
Plasmid#176323PurposepAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)DepositorInsertshuman codon optimized Cas13bt1 (catalytically inactivated)
huADAR2dd(E488Q) (ADARB1 Human)
Cas13bt1 crRNA + BpiI cloning site
UseAAV and CRISPRTags3xHA and HIV NESExpressionMammalianMutationE488Q, only the deaminase domain (aa 276-702 are …PromoterEFS (short EF1alpha) and hU6Available SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB-CuR-hSOD1
Plasmid#232477PurposeThe inducible PiggyBac Cumate Switch vector (PBQM812A-1 System Biosciences) expressing human SOD1gene including flag -tag (DYKDDDDK) on the 3' endDepositorInsertHomo sapiens superoxide dismutase 1 (SOD1 Human)
UseTransposon systemTagsDYKDDDDK (FLAG-tag)ExpressionMammalianPromoterCMV-CuOAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1–110)-IRES-Neomycin
Plasmid#223959PurposeDual fluorescent reporter for histone H2B and for APC/C activityDepositorUseLentiviralTagsiRFP670 and mCeruleanExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB-TR-hSOD1
Plasmid#232478PurposeTetracycline inducible PiggyBac vector expressing human SOD1gene including flag-tag (DYKDDDDK) on the 3' endDepositorInsertHomo sapiens superoxide dismutase 1 (SOD1 Human)
UseTransposon systemTagsDYKDDDDK (FLAG-tag)ExpressionMammalianPromoterCMV-TetOAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-TEAD4-YBD
Plasmid#166450PurposeExpresses fusion of mCherry and TEAD4-YAP binding domainDepositorAvailable SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX311-SHOC2-V5
Plasmid#134521PurposeLentiviral plasmid expressing SHOC2 with V5 C-term tagDepositorInsertSHOC2 (SHOC2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationSilent mutations made at wobble base position at …PromoterEF1alphaAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT230 lenti pEF-rTetR(SE-G72P)-3XFLAG-NCOA3-TAD-T2A-mCherry-BSD-WPRE
Plasmid#188763PurposeLentiviral backbone containing NCOA3 as a fusion with rTetR(SE-G72P)-3XFLAGDepositorInsertNCOA3 (NCOA3 Human)
UseLentiviralTagsrTetR(SE-G72P)-3xFLAGExpressionMammalianPromoterpEF1aAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Cry2-mCherry-TEAD4-IDR
Plasmid#166449PurposeExpresses fusion of CRY2PHR with mCherry and TEAD4-IDRDepositorAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-AMBRA1-501*-3xFLAG
Plasmid#174160PurposePiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 501DepositorInsertAMBRA1 (AMBRA1 Human)
Tags3xFLAGExpressionMammalianMutationMissing aa 501-1208PromoterEF1AAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-AMBRA1-217*-3xFLAG
Plasmid#174161PurposePiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 217DepositorInsertAMBRA1 (AMBRA1 Human)
Tags3xFLAGExpressionMammalianMutationMissing aa 217-1208PromoterEF1AAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT222 lenti pEF-rTetR(SE-G72P)-3XFLAG-FOXO3 FOXO-TAD-T2A-mCherry-BSD-WPRE
Plasmid#188760PurposeLentiviral backbone containing FOXO3 FOXO-TAD as a fusion with rTetR(SE-G72P)-3XFLAGDepositorInsertFOXO3 FOXO-TAD (FOXO3 Human)
UseLentiviralTagsrTetR(SE-G72P)-3xFLAGExpressionMammalianPromoterpEF1aAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT226 lenti pEF-rTetR(SE-G72P)-3XFLAG-SMARAC2 QLQ-T2A-mCherry-BSD-WPRE
Plasmid#188761PurposeLentiviral backbone containing SMARCA2 QLQ as a fusion with rTetR(SE-G72P)-3XFLAGDepositorInsertSMARCA2 QLQ (SMARCA2 Human)
UseLentiviralTagsrTetR(SE-G72P)-3xFLAGExpressionMammalianPromoterpEF1aAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT228 lenti pEF-rTetR(SE-G72P)-3XFLAG-KIBRA WW-T2A-mCherry-BSD-WPRE
Plasmid#188762PurposeLentiviral backbone containing KIBRA WW as a fusion with rTetR(SE-G72P)-3XFLAGDepositorInsertKIBRA WW (WWC1 Human)
UseLentiviralTagsrTetR(SE-G72P)-3xFLAGExpressionMammalianPromoterpEF1aAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT236 lenti pEF-rTetR(SE-G72P)-3XFLAG-CXXC1 PHD-T2A-mCherry-BSD-WPRE
Plasmid#188764PurposeLentiviral backbone containing CXXC1 PHD as a fusion with rTetR(SE-G72P)-3XFLAGDepositorInsertCXXC1 PHD (CXXC1 Human)
UseLentiviralTagsrTetR(SE-G72P)-3xFLAGExpressionMammalianPromoterpEF1aAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only