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We narrowed to 16,278 results for: nes

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Showing: 9221 - 9240 of 16278 results

  5. PB-PE SpG

    Plasmid
    #179081
    Purpose
    NOTE: An improved version of this plasmid is now available. See Addgene plasmid #242072. All-in-one prime editor piggyBac transposon, SpG variant
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R
    Promoter
    CAG
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179081-plasmid-map-sequence-id-352652

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  6. PB-PE xCas9

    Plasmid
    #172652
    Purpose
    All-in-one prime editor piggyBac transposon (xCas9)
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    xCas9_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    172652-plasmid-map-sequence-id-346754

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  7. pKIF1C-BioID2-HA

    Plasmid
    #130980
    Purpose
    Expression of HA-tagged KIF1C-BioID2
    Depositor
    Anne Straube
    Publication
    Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KIF1C-BioID2-HA (KIF1C Human)
    Tags
    BioID2-HA
    Expression
    Mammalian
    Mutation
    5 silent mutations that make this construct RNAi …
    Promoter
    CMV
    Available since
    Oct. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    130980-plasmid-map-sequence-id-259358

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  8. Mouse 3' Hp1a AID GFP PuroR

    Plasmid
    #127897
    Purpose
    HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Hp1a Gene
    Depositor
    Mark Groudine
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Hp1a (Cbx5 Mouse, Mustard Weed)
    Use
    Donor plasmid
    Tags
    GFP, AID, PuroR
    Mutation
    Inserting GFP AID 2A Puro into mouse 3' Hp1a
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127897-plasmid-map-sequence-id-250990

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  9. pcDNA 3.1 SIRT1-deltaExon2

    Plasmid
    #105671
    Purpose
    Mammalian expression construct of mouse SIRT1 short isoform (lacking Exon2)
    Depositor
    Ullas Kolthur-Seetharam
    Publication
    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SIRT1 (Sirt1 Mouse)
    Expression
    Mammalian
    Mutation
    Synonymous base changes not affecting the protein…
    Promoter
    CMV
    Available since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    105671-plasmid-map-sequence-id-265025

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  10. CFF-EGFP

    Plasmid
    #86061
    Purpose
    A mammalian expression plasmid encoding fibrocystin mimentic clone CFF (CD8a luminal domain, fibrocystin transmembrane and cytosolic domains) with C-terminus EGFP.
    Depositor
    Lei Lu
    Publication
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fibrocystin (Pkhd1 Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains luminal and transmembrane domains of CD8…
    Promoter
    CMV
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86061-plasmid-map-sequence-id-177941

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  11. CD8a-cNLS-EGFP

    Plasmid
    #86052
    Purpose
    A mammalian expression plasmid expressing CD8a luminal and transmembrane domains followed by cNLS (classical nuclear localization signal) and C-terminus EGFP.
    Depositor
    Lei Lu
    Publication
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    CD8a (CD8A Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    CD8a luminal and transmembrane along with two SV4…
    Promoter
    CMV
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86052-plasmid-map-sequence-id-177916

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  13. pcDNA3.1 Flag YFP hNKCC1 A734C (NT508)

    Plasmid
    #50888
    Purpose
    Expresses human NKCC1 A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    A734C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50888-plasmid-map-sequence-id-76361

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Showing: 9221 - 9240 of 16278 results