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PB-BE3-3NLS

Plasmid

#119032

Purpose

Stably express BE3 in mammalian cells

Depositor

Jinsong Li

Article

Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

Insert

BE3

Tags

3xFlag

Expression

Mammalian

Available Since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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CHL1 wt-eGFP

Plasmid

#117876

Purpose

protein expression

Depositor

Geoffrey Chang

Insert

CHL1 wt-eGFP (AT5G40090 Mustard Weed)

Expression

Yeast

Promoter

AOX

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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CAG-loxP-3xpA-H2BsfGFP-tdTomato - opt GS PQR Var3 P2A -APGST E

Plasmid

#113847

Purpose

Histone H2B-superfolder GFP and tdTomato linked by P2A (-APGST linker)

Depositor

Mario Capecchi

Insert

H2B-sfGFP-P2A-tdTomato

Expression

Mammalian

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV6-XL4 FLAG-CBLN3-Fc

Plasmid

#115768

Purpose

Expression of the extracellular domain of CBLN3 with N-terminal FLAG tag and C-terminal hIgG tag.

Depositor

Davide Comoletti

Insert

CBLN3 (CBLN3 Human)

Tags

FLAG and hIgG/Fc

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR hH1-ARRET

Plasmid

#111382

Purpose

ARRET with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences under control of hH1 promoter and terminator

Depositor

Klaus Holzmann

Insert

hH1-ARRET

Use

Gateway entry cloning

Promoter

hH1

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBCSK_HIS3_I

Plasmid

#109058

Purpose

yeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.

Depositor

Colin Logie

Insert

HIS3 (HIS3 Budding Yeast, Zygosaccharomyces rouxii)

Use

Cre/Lox

Expression

Bacterial and Yeast

Promoter

HIS3

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pBCSK_HIS3_II

Plasmid

#109059

Purpose

yeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.

Depositor

Colin Logie

Insert

HIS3 (HIS3 Budding Yeast, Zygosaccharomyces rouxii)

Use

Cre/Lox

Expression

Bacterial and Yeast

Promoter

HIS3

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-BE3-PGK-Puro

Plasmid

#110842

Purpose

Lentiviral vector for constitutive expression of BE3 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3

Use

Lentiviral

Mutation

D10A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pHMR1B

Plasmid

#102958

Purpose

gene cloning of MR1B

Depositor

Gennaro De Libero, Lucia Mori

Insert

MR1B cDNA (MR1P1 Human)

Use

Cloning

Mutation

Stop codon has been removed by HindIII site

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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BRD1A

Plasmid

#74666

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

BRD1A (BRD1 Human)

Tags

His-6-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
mTagRFP-T2-Tubulin-6

Plasmid

#58044

Purpose

Localization: Microtubules, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Insert

Tubulin (TUBA1B Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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psicheck_NDUFA6_3UTR

Plasmid

#65886

Purpose

Luciferase assay vector, assessing regulation of NDUFA6 3' UTR

Depositor

Thomas Tuschl

Insert

NDUFA6 (NDUFA6 Human)

Expression

Mammalian

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
PAS529 (TJF072)

Plasmid

#65603

Purpose

pETDuet-1 Containing FadD Q338R-His

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Tags

6x His

Expression

Bacterial

Mutation

Q338R

Promoter

T7 lacO

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
mCherry-Espin-Like-C-18

Plasmid

#62744

Purpose

Localization: Actin Bundling , C1 Cloning Vector, Excitation:587, Emission:610

Depositor

Michael Davidson

Insert

EspinLike

Tags

mCherry

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

March 10, 2015

Availability

Academic Institutions and Nonprofits only
mPlum-H1-10

Plasmid

#55973

Purpose

Localization: Nucleus/Histones, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Insert

H1 (H1f0 Mouse)

Tags

mPlum

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
Dronpa-SSTR3-N-17

Plasmid

#57296

Purpose

Localization: Cilium, Excitation: 503, Emission: 518

Depositor

Michael Davidson

Insert

SSTR3

Tags

Dronpa

Expression

Mammalian

Mutation

A270V in SSTR3

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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tdEos-ARP2-N-14

Plasmid

#57581

Purpose

Localization: Actin Related Protein, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

ARP2 (ACTR2 Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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tdEos-ARP3-C-12

Plasmid

#57582

Purpose

Localization: Actin Related Protein, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

ARP3 (ACTR3 Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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tdEos-VASP-N-10

Plasmid

#57686

Purpose

Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

VASP

Tags

tdEos

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mCitrine-VASP-5

Plasmid

#56571

Purpose

Localization: Actin/Focal Adhesions, Excitation: 516, Emission: 529

Depositor

Michael Davidson

Insert

VASP

Tags

mCitrine

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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