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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,891 results for: Coli

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Showing: 9261 - 9280 of 9891 results

  4. pDule-para-aminoPhe

    Plasmid
    #85502
    Purpose
    Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.
    Insert
    p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32T, E107T, D158P, I159L, L162A
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85502-plasmid-map-sequence-id-169860

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  5. sUPRa

    Plasmid
    #223242
    Purpose
    sUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levels
    Depositor
    Colin Akerman
    Article
    Chakrabarty et al Sci Rep. 2024 Jul 1;14(1):14990. doi: 10
    Insert
    sUPRa
    Use
    AAV
    Promoter
    Custom
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    223242-plasmid-map-sequence-id-450772

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  7. pECNUS4

    Plasmid
    #184887
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Article
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    RPS5A
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    184887-plasmid-map-sequence-id-437896

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  14. pAD-KPS12-Pman

    Plasmid
    #133832
    Purpose
    Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.
    Depositor
    Oscar Kuipers
    Article
    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57
    Insert
    mKate(KPS12)
    Use
    E.coli/bacillus spp. shuttle vector
    Expression
    Bacterial
    Promoter
    Pman
    Available Since
    Nov. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    133832-plasmid-map-sequence-id-261722

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  16. pDule2-Acd82

    Plasmid
    #174100
    Purpose
    Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.
    Depositor
    Ryan Mehl
    Article
    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.
    Insert
    Acridone 82 tRNA synthetase and tRNA
    Expression
    Bacterial
    Mutation
    N311S, C313G, V366A, W382T
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    174100-plasmid-map-sequence-id-345717

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  18. pDule-Acd82

    Plasmid
    #174099
    Purpose
    Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.
    Depositor
    Ryan Mehl
    Article
    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.
    Insert
    Acridone 82 tRNA synthetase and tRNA
    Expression
    Bacterial
    Mutation
    N311S, C313G, V366A, W382T
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    174099-plasmid-map-sequence-id-345716

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  19. pIND(V5)EcoDam

    Plasmid
    #59201
    Purpose
    Mammalian DamID vector used to fuse gene of interest to V5-tagged E.coli Dam. Can also express Dam alone as negative control.
    Depositor
    Bas van Steensel
    Article
    Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep
    Type
    Empty backbone
    Use
    Damid
    Tags
    Dam (DNA adenine methyltransferase) and V5
    Expression
    Mammalian
    Promoter
    Heat Shock Minimal Promoter
    Available Since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    59201-plasmid-map-sequence-id-247615

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Showing: 9261 - 9280 of 9891 results