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Omega Destination-CMV::ELuc:bGH

Plasmid

#124528

Purpose

Omega destination vector, that has a CMV:ELUC:bgH in the backbone

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pNES-iRFP-C1

Plasmid

#116863

Purpose

cytosolic iRFP

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

iRFP713

Tags

X.leavis map2k1.L(32-44)

Expression

Mammalian

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:(MCS)-S11-DI-GFP1–9

Plasmid

#108260

Purpose

Binary vectors used for the inducible expression of dual intein-coupled tripartite split-sfGFP components in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-V5-hTEV

Plasmid

#65800

Purpose

expresses human codon-optimised TEV protease

Depositor

Douglas Green

Article

Oberst et al J Biol Chem. 2010 May 28;285(22):16632-4

Insert

V5-human TEV

Tags

V5

Expression

Mammalian

Mutation

codon-optimised to express in mammalian cells

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pFP.R267

Plasmid

#138179

Purpose

Expresses the split mCherry reporter interrupted by the gp41-1 intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(SGY).gp41-1.(SSS)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCMU-LEr

Plasmid

#61185

Purpose

Fluorescent reporter for late endosome mCherry-MtRAB5A2 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB5A2

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCDM8 hCD81

Plasmid

#11588

Depositor

Shoshana Levy

Article

Oren et al Mol Cell Biol. 1990 Aug . 10(8):4007-15.

Insert

CD81 (CD81 Human)

Expression

Mammalian

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
pCAG-SRGAP3-EGFP

Plasmid

#168504

Purpose

Expression of full length SRGAP3 with a C-terminal EGFP fusion

Depositor

Franck Polleux

Article

Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

Insert

SRGAP3 (SRGAP3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBN1

Plasmid

#86710

Purpose

C. elegans injection marker

Depositor

Peter Askjaer

Article

Rodenas et al Mol Biol Cell. 2012 Mar;23(5):930-44. do

Insert

Plmn-1::mCherry::his-58::pie-1 3'UTR

Expression

Worm

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-CoChR-mRuby2-ST

Plasmid

#109131

Purpose

Cation channelrhodopsin CoChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

CoChR-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a(+)- PC-MjMAT

Plasmid

#179372

Purpose

PC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.

Depositor

Andrea Rentmeister

Article

Peters et al Chembiochem. 2021 Oct 4. doi: 10.1002/cb

Insert

PC-MjMAT

Tags

6xHis

Expression

Bacterial

Mutation

L147A, I351A

Promoter

T7

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-GFP-SF3B6-FLAG

Plasmid

#86870

Purpose

To express SF3B6 with a GFP and FLAG tags at N- and C-terminus, respectively.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

SF3B6 (SF3B6 Human)

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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GLI K12

Plasmid

#16419

Depositor

Bert Vogelstein

Article

Kinzler et al Nature. 1988 Mar 24. 332(6162):371-4.

Insert

GLI (GLI1 Human)

Expression

Bacterial

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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pRU319

Plasmid

#167686

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::spCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 aa 1-552

Plasmid

#74614

Purpose

Expresses a truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-552 (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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6xHis(TEV)-Drp1-StrepII

Plasmid

#174422

Purpose

Expresses human Drp1 Isoform 3 in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Tags

6xHis(TEV) and StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pECFP-histone H3

Plasmid

#179698

Purpose

visualization of nucleus/chromosome

Depositor

Kenji Sugimoto

Article

Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

Insert

Histone H3 (H3c10 Mouse)

Tags

ECFP

Expression

Mammalian

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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GFPZFN1.4-B2H

Plasmid

#26791

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

GFP-ZFN1

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
His6-TEV-Venus-pBluescript

Plasmid

#166756

Purpose

For purification of Venus fluorophore

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

Venus

Tags

6x Histidine tag, and TEV protease site

Expression

Bacterial

Promoter

T7

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pEG BacMam N term His8 GFP

Plasmid

#160680

Purpose

vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

His8 GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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