We narrowed to 10,634 results for: SEC
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Plasmid#157066PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only
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Donor_N_FH_Piwi
Plasmid#186651PurposeDonor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.DepositorInsertOSS PIWI N-terminal 3xFlag-3xHA Tag Donor Plasmid (piwi Fly)
Tags3xFlag-3xHAExpressionInsectAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
S+Q111R+N122D
Plasmid#167175PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R and N122D mutations in Sf9 cellsDepositorInsertsATP1A1-S+Q111R+N122D
ATP1B1
ExpressionBacterial and InsectMutationChanged Q at 111 to R and changed N at 122 to DPromoterP10 and PHAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
S+10subs
Plasmid#167176PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 10 R mutations in Sf9 cellsDepositorInsertsATP1A1-S+10R
ATP1B1
ExpressionBacterial and InsectMutationChanged A at 112 to T; E at 116 to D; I at 135 to…PromoterP10 and PHAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
S+12subs
Plasmid#167177PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 12 R mutations in Sf9 cellsDepositorInsertsATP1A1-S+12R
ATP1B1
ExpressionBacterial and InsectMutationChanged Q at 111 to R; A at 112 to T; E at 116to …PromoterP10 and PHAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAW-BiP-Nb127D01-mCherry-KDEL
Plasmid#171575PurposeExpresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membraneDepositorInsertBiP-Nb127D01-mCherry-KDEL (CXCR2 Human)
TagsBiP signal peptide and mCherry-KDELExpressionInsectPromoterfly actin5C promoterAvailable SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW-BiP-NbVHH05-mCherry-KDEL
Plasmid#171574PurposeExpresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membraneDepositorInsertBiP-NbVHH05-mCherry-KDEL (UBE2J1 Human)
TagsBiP signal peptide and mCherry-KDELExpressionInsectPromoterfly actin5C promoterAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_1-590 POLI
Plasmid#131233PurposeMammalian expression of DNA polymerase iota residues 1-590 with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains a 24 bp insertion within the Pol iota co…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_1-420 POLI
Plasmid#131234PurposeMammalian expression of DNA polymerase iota residues 1-420 with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains a 24 bp insertion within the Pol iota co…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
R979-X57-008: Hs.KRAS4b(1-169)
Plasmid#159690PurposeBaculovirus protein expression of KRAS4b (1-169)DepositorInsertHs.KRAS4b(1-169) (KRAS Human)
ExpressionInsectAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-chiRNA_dCrkgRNA1
Plasmid#131139PurposeEncodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).DepositorAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-chiRNA_dCrkgRNA2
Plasmid#131140PurposeEncodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).DepositorAvailable SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Rescue myc-PCDHGC5 mRNA
Plasmid#122229PurposeRescue mRNA for Protocadherin gamma C5 knock down with sh1 shRNA (plasmid 122227). With five silent mutations at sh1 shRNA target site.DepositorInsertPCDHGC5 (Pcdhgc5 Rat)
Tags9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…ExpressionMammalianMutationSilent mutations are in five consecutive codons …Available SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMCh-646
Plasmid#92289PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED 7W7A with seven mutated W-motifs in D. melanogaster cellsDepositorInsertdGW182 CED 7W7A (gw Fly)
TagsNHAExpressionInsectMutationdGW182 CED: aa 940-1215 with the following point …PromoterAc5Available SinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAc-GFP-dSUMOdeltaC
Plasmid#85697Purposeexpression of GFP-SUMO1 delta C in Drosophila cellsDepositorInsertSUMO (Sumo Fly)
TagsGFPExpressionInsectMutationnon-conjugatable SUMO, lacking the C-terminal gly…PromoterpAc5Available SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-NSF/T646A
Plasmid#74937PurposeMammalian expression of human NSF mutant T646A (mutation in the D2 domain of the protein)DepositorInsertNSF (NSF Human)
TagsFLAGExpressionMammalianMutationT646A (mutation in the D2 domain of the protein)PromoterCMVAvailable SinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-NSF/T645A
Plasmid#74936PurposeMammalian expression of human NSF mutant T645A (mutation in the D2 domain of the protein)DepositorInsertNSF (NSF Human)
TagsFLAGExpressionMammalianMutationT645A (mutation in the D2 domain of the protein)PromoterCMVAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-LBP-Fc(DAPA)-AviTag-6xHis
Plasmid#156870PurposeMammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.DepositorInsertLBP (LBP Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA3B-Fc(DAPA)-AviTag-6xHis
Plasmid#156956PurposeMammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.DepositorInsertSEMA3B (SEMA3B Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(Gaussia Luciferase)#5 in pTwist-CMV
Plasmid#216164PurposeExpression of secreted Gaussia Luciferase specifically in cells with TDP-43 loss of function. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor CommentsDepositorInsertGaussia princeps luciferase (with double mutation to improve stability)
ExpressionMammalianAvailable SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only