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pCRII-TOPO-DrSmg7_F

Plasmid

#146280

Purpose

Bacterial Expression of DrSmg7

Depositor

Elisa Izaurralde

Publication

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

DrSmg7 (smg7 Zebrafish)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO-DrUPF3a_F

Plasmid

#146283

Purpose

Bacterial Expression of DrUPF3a

Depositor

Elisa Izaurralde

Publication

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

DrUPF3a (upf3a Zebrafish)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubgenomic-PTC62_D

Plasmid

#146145

Purpose

Mammalian Expression of tubgenomic-PTC62

Depositor

Elisa Izaurralde

Publication

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubgenomic-PTC62

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubgenomic-PTC90_D

Plasmid

#146147

Purpose

Mammalian Expression of tubgenomic-PTC90

Depositor

Elisa Izaurralde

Publication

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubgenomic-PTC90

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubgenomic-PTC130_D

Plasmid

#146148

Purpose

Mammalian Expression of tubgenomic-PTC130

Depositor

Elisa Izaurralde

Publication

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubgenomic-PTC130

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pE5771

Plasmid

#170778

Purpose

production of tagged outer membrane protein

Depositor

Anthony Schryvers

Publication

Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

Insert

Kan resistance (kanR )

Tags

polyhisTEVSBPTEV

Expression

Bacterial

Promoter

T7

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYI006

Plasmid

#170840

Purpose

FRO6p::GUSPlus::tOCS in pFP100

Depositor

Christian Fankhauser

Publication

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

FRO6p::GUSPlus::tOCS

Expression

Plant

Promoter

FRO6

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe3-Venus-RfA

Plasmid

#186371

Purpose

Destination vector for the expression of a fluorescent fusion of N-ter Venus and a protein of interest into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Publication

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-H2B-mTurbo

Plasmid

#187178

Purpose

Express histone H2B tagged with miniTurbo under the control of CAG promoter

Depositor

Isei Tanida

Publication

Sanada et al Sci Rep. 2022 Jul 1;12(1):11130. doi: 10

Insert

histone H2B fused with miniTurbo (BirA mutant)

Expression

Mammalian

Promoter

CAG

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubgenomic-PTC33_D

Plasmid

#146144

Purpose

Mammalian Expression of tubgenomic-PTC33

Depositor

Elisa Izaurralde

Publication

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubgenomic-PTC33

Expression

Mammalian

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pX458_sgRNA_Ago1_5

Plasmid

#172470

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1

Depositor

Constance Ciaudo

Publication

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

sgAgo1

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pX458_sgRNA_Ago1_6

Plasmid

#172471

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1.

Depositor

Constance Ciaudo

Publication

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

sgAgo1

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-GFP-ECL-3TM-hcvs-KKMP

Plasmid

#179641

Purpose

HCVP-inducible membrane-bound GFP RELEASE construct

Depositor

Xiaojing Gao

Publication

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Membrane-bound GFP RELEASE

Expression

Mammalian

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pTK73_htz-1

Plasmid

#174546

Purpose

htz-1 targeting gRNA expression

Depositor

Masato Kanemaki

Publication

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Insert

htz-1 targeting gRNA

Expression

Worm

Promoter

Ce-U6 promoter

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBN079

Plasmid

#167960

Purpose

express E. coli E69 (O9a:K30) Wzc with Y718E

Depositor

James Naismith

Publication

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y718E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y718E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN072

Plasmid

#167953

Purpose

express E. coli E69 (O9a:K30) Wzc with Y705E

Depositor

James Naismith

Publication

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y705E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y705E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN073

Plasmid

#167954

Purpose

express E. coli E69 (O9a:K30) Wzc with Y706E

Depositor

James Naismith

Publication

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y706E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y706E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN075

Plasmid

#167956

Purpose

express E. coli E69 (O9a:K30) Wzc with Y708E

Depositor

James Naismith

Publication

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y708E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y708E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN076

Plasmid

#167957

Purpose

express E. coli E69 (O9a:K30) Wzc with Y713E

Depositor

James Naismith

Publication

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y713E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y713E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN077

Plasmid

#167958

Purpose

express E. coli E69 (O9a:K30) Wzc with Y715E

Depositor

James Naismith

Publication

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y715E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y715E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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