We narrowed to 15,730 results for: NOL

1. pMonAID_nCas9-PmCDA_Hyg_ALS

   Plasmid

   #91693

   Purpose

   Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A for nickase Cas9

   Promoter

   2x35S

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Dnase1L3 R206C pIRES-DsRed

   Plasmid

   #98847

   Purpose

   Expresses Human Dnase1L3 with activity-reducing HUVS mutation R206C in mammalian cells along with DsRed

   Depositor

   Peter Keyel

   Article

   Shi et al Front Immunol. 2017 May 8;8:522. doi: 10

   Insert

   Dnase1L3 (DNASE1L3 Human)
   

   Tags

   IRES DsRed

   Expression

   Mammalian

   Mutation

   R206C which greatly reduces nuclease activity

   Promoter

   CMV

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_UdgX-APOBEC1-UdgX-HF-nCas9

   Plasmid

   #163559

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-APOBEC1-UdgX-HF-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. B*40:01:01

   Plasmid

   #203257

   Purpose

   Overexpress HLA allele

   Depositor

   Derin Keskin, Catherine Wu

   Article

   Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

   Insert

   B*40:01:01 (HLA-B Human)
   

   Expression

   Mammalian

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTol2-10xUAS-Voltron-ST

   Plasmid

   #119040

   Purpose

   UAS-driven expresion of soma-localized Voltron in zebrafish

   Depositor

   Eric Schreiter

   Article

   Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

   Insert

   Voltron-ST
   

   Use

   Zebrafish expression

   Promoter

   E1b

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNLF1-C_hCOL4A5-Nluc_R1683X

   Plasmid

   #198124

   Purpose

   Express hCOL4A5_R1683X in mammalian cells

   Depositor

   Jeffrey Miner

   Article

   Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

   Insert

   COL4A5 (COL4A5 Human)
   

   Tags

   Nluc

   Expression

   Mammalian

   Mutation

   R1683X

   Promoter

   CMV, beta-globin/IgG chimeric intron

   Available Since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-TRE3G-BE3RA-PGK-Puro

   Plasmid

   #110846

   Purpose

   Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA
   

   Use

   Lentiviral

   Mutation

   D10A

   Promoter

   TRE3G

   Available Since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. non-targeting control gRNA (BRDN0001145598)

   Plasmid

   #80173

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. p2T-cmv-SpCas9-TadCBEa-BlastR

   Plasmid

   #193847

   Purpose

   Express TadCBEa in mammalian cells with BlastR selection, Tol2 integration

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadCBEa
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. MLKL gRNA (BRDN0001148608)

    Plasmid

    #77539

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MLKL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MLKL (MLKL Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. MLKL gRNA (BRDN0001146456)

    Plasmid

    #77540

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MLKL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MLKL (MLKL Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. K15-EGFP

    Plasmid

    #44266

    Depositor

    George Cotsarelis

    Article

    Morris et al Nat Biotechnol. 2004 Apr;22(4):411-7. Ep

    Insert

    K15 promoter (-4.8) (Krt15 Mouse)
    

    Use

    Mouse Targeting; Fluorescence microscopy

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Contains the murine K15 promoter (-4.8) fragment

    Promoter

    murine K15 promoter (-4.8) fragment

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

13. SFFV-AID-CTERM-GFP

    Plasmid

    #185768

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, AID

    Mutation

    none

    Promoter

    SFFV

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNAD1

    Plasmid

    #154347

    Purpose

    pUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistance

    Depositor

    John van der Oost

    Article

    Naduthodi et al Biotechnol Biofuels. 2019 Mar 25;12:66.

    Insert

    ShBle
    

    Use

    Microalgae

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Violaxanthin Chlorophyll a Binding Protein Precur…

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. SARS_COV2_ORF3a_HA_BASU

    Plasmid

    #153994

    Purpose

    Express Orf3a gene along with HA and BASU

    Depositor

    Paul Khavari

    Article

    Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

    Insert

    Orf3a (ORF3a Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSF-Ivy-his-hLYZ

    Plasmid

    #83920

    Purpose

    Co-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment.

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Ivy
    lysozyme (LYZ Human)
    

    Tags

    Ivy protein has C-terminal hisx6 tag

    Expression

    Bacterial

    Mutation

    Ivy leader sequence deleted and replaced.

    Promoter

    T7 and t7

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pL-CRISPR.SFFV.PAC

    Plasmid

    #57829

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-PAC
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-hA3A-BE3-Y130F

    Plasmid

    #113428

    Purpose

    Expresses hA3A-BE3-Y130F in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3A-BE3-Y130F (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_Y130F

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. MLKL gRNA (BRDN0001149268)

    Plasmid

    #77541

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MLKL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MLKL (MLKL Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. TV-hPITX3-forward

    Plasmid

    #22073

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    PITX3 (ASMD Human)
    

    Use

    Cre/Lox; Zfn donor

    Tags

    eGFP

    Available Since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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