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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,351 results for: multi

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Showing: 9361 - 9380 of 18351 results

  1. pcDNA5/FRT/TO_TOMM20-STf

    Plasmid
    #182002
    Purpose
    CMV overexpression of SNAP-tag fast at the mitochondria surface of mammalian cell lines
    Depositor
    Kai Johnsson
    Publication
    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TOMM20-STf
    Expression
    Mammalian
    Mutation
    Fast mutation on SNAP-tag E30R
    Promoter
    CMV
    Available since
    Nov. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    182002-plasmid-map-sequence-id-356088

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  4. pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry

    Plasmid
    #170182
    Purpose
    Mammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptide
    Depositor
    Hunter Fraser
    Publication
    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Tags
    3XFlag and T2A
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    170182-plasmid-map-sequence-id-333578

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  5. pNOC_dCas9_sgRNA NC

    Plasmid
    #176259
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and
    Depositor
    John van der Oost
    Publication
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176259-plasmid-map-sequence-id-346581

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  10. pCK351

    Plasmid
    #129696
    Purpose
    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120034435
    Depositor
    John Heap
    Publication
    Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m
    Inserts
    PrhaBAD
    yfp
    rhaS
    Terminator ECK120034435
    Expression
    Bacterial
    Available since
    Oct. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    129696-plasmid-map-sequence-id-258483

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  11. pcDNA3.1(+)-blueAKAR(T/A)

    Plasmid
    #118471
    Purpose
    Negative-control mutant for blueAKAR biosensor.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    blueAKAR(T/A)
    Tags
    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Mutation
    Contains Thr-to-Ala mutation in substrate sequenc…
    Promoter
    CMV
    Available since
    April 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    118471-plasmid-map-sequence-id-243089

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  12. pcDNA3.1(+)-ExRai-AKAR-Kras

    Plasmid
    #118408
    Purpose
    Plasma membrane-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ExRai-AKAR-Kras
    Tags
    C-terminal targeting sequence from Kras
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    118408-plasmid-map-sequence-id-242822

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  13. pJEP311-pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid
    #113688
    Purpose
    SaCas9 driven by EFS. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.
    Depositor
    Jonathan Ploski
    Publication
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Insert
    SaCas9 (NEWENTRY )
    Use
    AAV and CRISPR
    Tags
    Flag, HAx2, KASH, and NLS
    Promoter
    Cytomegalo Virus(CMV)
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113688-plasmid-map-sequence-id-231828

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  14. pJEP312-pAAV-CMV-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid
    #113689
    Purpose
    SaCas9 driven by CMV. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS
    Depositor
    Jonathan Ploski
    Publication
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Insert
    SaCas9 (NEWENTRY )
    Use
    AAV and CRISPR
    Tags
    Flag, HAx2, KASH, and NLS
    Promoter
    Cytomegalo Virus(CMV)
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    113689-plasmid-map-sequence-id-231823

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  15. pcDNA3-blueCKAR(T/A)

    Plasmid
    #118473
    Purpose
    Negative-control mutant for blueCKAR biosensor.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    blueCKAR(T/A)
    Tags
    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Mutation
    Contains Thr-to-Ala mutation in substrate sequenc…
    Promoter
    CMV
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    118473-plasmid-map-sequence-id-230294

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  16. pAAV hSyn1 bPAC cMyc T2A tDimer

    Plasmid
    #85397
    Purpose
    humanized photoactivated adenylyl cyclase with cMyc Tag driven by neuron-specific promoter, plus red fluorescent protein
    Depositor
    Thomas Oertner
    Inserts
    humanized photoactivated adenylyl cyclase
    red fluorescent protein
    Use
    AAV
    Tags
    cMyc Tag
    Expression
    Mammalian
    Promoter
    human synapsin-1 and ribosomal skip sequence T2A
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    85397-plasmid-map-sequence-id-201306

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  19. pcDNA-NLS-ZapCmR1.1

    Plasmid
    #59011
    Purpose
    Nuclear zinc-binding FRET biosensor
    Depositor
    Amy Palmer
    Publication
    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-ZapCmR1.1 genetically encoded Zn(II) sensor
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    See comment
    Promoter
    CMV
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 9361 - 9380 of 18351 results