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JC021

Plasmid

#104944

Purpose

Estrogen inducing G-CSF

Depositor

Timothy Lu

Article

Cao et al Nat Commun. 2018 Jan 8;9(1):77. doi: 10.

Insert

G-CSF

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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Human Genome-wide Intron Tagging Library, Frame 0

Pooled Library

#216133

Purpose

Library of intron-targeting sgRNAs for pooled tagging of proteins with GFP or other protein tags at their endogenous genomic locus using an intron tagging approach.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only
pWS3867

Plasmid

#185744

Purpose

Sub-array backbone 3/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJH104

Plasmid

#68384

Purpose

Integration vector for creating Sinorhizobium meliloti Landing Pad Strain

Depositor

Trevor Charles

Insert

attP-aadA-uidA-attP

Use

Synthetic Biology

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pHM130

Plasmid

#225529

Purpose

Encodes destablized GFP under IBRE4 promoter, and constitutive BFP

Depositor

Jared Toettcher

Article

McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

Inserts

GFP
BFP

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_nLightR

Plasmid

#217657

Purpose

Expresses norepinephrine indicator nLightR under mammalian promoter

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Insert

nLightR

Expression

Mammalian

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pJ2040

Plasmid

#140679

Purpose

NOT gate with sg-4

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

NOT gate with sg-4

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pJ2043

Plasmid

#140677

Purpose

NOT gate with sg-2

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

NOT gate with sg-2

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pJ2039

Plasmid

#140678

Purpose

NOT gate with sg-3

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

NOT gate with sg-3

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBP

Plasmid

#72947

Purpose

Backbone vector with internal BsmBI removed by site-directed mutagenesis

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMYT096

Plasmid

#198867

Purpose

Transient CRISPR/Cas9 Vector without a selectable marker to be used in the Ellis MYT system

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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p63_AF043

Plasmid

#175878

Purpose

arabinose inducible luxI, AHL inducible tetR, constitutive mCherry

Depositor

Chris Barnes

Article

Fedorec et al Nat Commun. 2021 Mar 30;12(1):1977. doi:

Insert

AF043 cassette

Use

Synthetic Biology

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJ2048_2xNOT

Plasmid

#140673

Purpose

Double-inverter

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

Double-inverter

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_mTFP1

Plasmid

#118858

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

mTFP1

Tags

His-tag

Expression

Bacterial

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pZA1pLtetO-GFP

Plasmid

#78227

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCH104

Plasmid

#72298

Purpose

Receptors for two-strain system

Depositor

Matthew Bennett

Article

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Inserts

RhlR (rhlR )
CinR

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pHM093

Plasmid

#225525

Purpose

Encodes rtTA under IBRE4 promoter, and constitutive BFP

Depositor

Jared Toettcher

Article

McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

Inserts

rtTA
BFP

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHM039

Plasmid

#225523

Purpose

Encodes Wnt-responsive rtTA and constitutive BFP

Depositor

Jared Toettcher

Article

McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

Inserts

rtTA
BFP

Expression

Mammalian

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAS166

Plasmid

#194489

Purpose

43-8 Op (Part 2)

Depositor

Ahmad Khalil

Article

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

43-8 Op (Part 2)

Use

Synthetic Biology

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pWS3806

Plasmid

#185747

Purpose

Sub-array spacer 2/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 2/4

Use

CRISPR

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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