Ready-to-use AAV Retrograde particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

expression of PABPC1-MS2 fusion, double mutant.

The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage

pCMV-myc with cDNA encoding HsTRAPPC10 aa residues 1000-1259

pCMV-myc with cDNA encoding HsTRAPPC11 aa residues 701-1133

Expression vector for a customizable guide RNA

E. coli - Veillonella shuttle plasmid with single selectable marker