We narrowed to 11,580 results for: Vars;

1. pAAV-CA-FLEX-dtA

   Plasmid

   #232236

   Purpose

   Expresses diphtheria toxin A subunit in Cre-positive cells.

   Depositor

   Naoshige Uchida

   Article

   Tsutsui-Kimura et al bioRxiv 2022.02.05.479267

   Insert

   Diphtheria toxin A subunit
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CA

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL2_163

   Plasmid

   #199095

   Purpose

   Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

   Depositor

   Cultivarium Tools, Charlie Gilbert, Nili Ostrov

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pAMβ1 ORI plus NGS barcode 3, gentamicin resistance cassette, mScarlet-I cassette, oriT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-6xHis-GFP(+12)

   Plasmid

   #199161

   Purpose

   used to express a supercharged GFP variant in E. coli

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   GFP(+12)
   

   Use

   Tags

   MGHHHHHHGGA

   Expression

   Mutation

   E16R, E44K, D86K, D112K, T115K, D127R, I138R, E15…

   Promoter

   T7

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. p458 VQR

   Plasmid

   #101727

   Purpose

   Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VQR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   VQR

   Promoter

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-6xHis-GFP(+18)

   Plasmid

   #199162

   Purpose

   used to express a supercharged GFP variant in E. coli

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   GFP(+18)
   

   Use

   Tags

   MGHHHHHHGGA

   Expression

   Mutation

   E16R, D29K, E42K, E44K, D86K, D112K, T115K, D127R…

   Promoter

   T7

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. mFGF8b

   Plasmid

   #22090

   Depositor

   Gail Martin

   Article

   Crossley et al Development. 1995 Feb . 121(2):439-51.

   Insert

   FGF8 (Fgf8 Mouse)
   

   Use

   Bacterial

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pSX2720

   Plasmid

   #200764

   Purpose

   Expresses Superfolder GFP-tag fused single change variable fragment antibody of GCN4 (Suntag) with the tissue-specific promoter col-19 in C. elegans.

   Depositor

   Suhong Xu

   Article

   Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

   Insert

   scFv-sfGFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   col-19

   Available Since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ii-Str_neomycin

   Plasmid

   #65308

   Purpose

   Luminal ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Invariant chain (p33)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Streptavidin in its lumenal part

   Promoter

   CMV

   Available Since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. LT3GEN

   Plasmid

   #111173

   Purpose

   Tet-regulated miR-E (miR-30 variant)-based RNAi

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   T3G

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV-CitroffRH

    Plasmid

    #158546

    Purpose

    Mammalian expression of nonbinding variant of inverse-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    CitroffRH
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available Since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL2_166

    Plasmid

    #199098

    Purpose

    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

    Depositor

    Cultivarium Tools, Charlie Gilbert, Nili Ostrov

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    RK2 ORI plus NGS barcode 12, gentamicin resistance cassette, mScarlet-I cassette, oriT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDEST14-DoubleCatcher-⍺-Lock

    Plasmid

    #216286

    Purpose

    Expresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher ⍺-Lock
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST14-DoubleCatcher-β-Lock

    Plasmid

    #216287

    Purpose

    Expresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher β-Lock
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST14-DoubleCatcher-γ-Lock

    Plasmid

    #216288

    Purpose

    Expresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher γ-Lock
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST14-DoubleCatcher-δ-Lock

    Plasmid

    #216289

    Purpose

    Expresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher δ-Lock
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST14-DoubleCatcher-ε-Lock

    Plasmid

    #216290

    Purpose

    Expresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher ε-Lock
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Ii-Str_puromycin

    Plasmid

    #65309

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Invariant chain (p33)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Streptavidin in its lumenal part

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIBA2-Int-SpyTag

    Plasmid

    #198038

    Purpose

    Expression plasmid for producing SpyTagged stalled secretion variant of intimin

    Depositor

    Jack Leo

    Article

    Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

    Insert

    eaeA
    

    Use

    Tags

    SpyTag

    Expression

    Bacterial

    Mutation

    lacks native signal peptide (amino acids 1-39)

    Promoter

    tet

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL3-LRF

    Plasmid

    #66936

    Purpose

    Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).

    Depositor

    Alvaro Monteiro

    Article

    Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV-40

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pGL2_167

    Plasmid

    #199099

    Purpose

    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

    Depositor

    Cultivarium Tools, Charlie Gilbert, Nili Ostrov

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pBBR1 ORI plus NGS barcode 13, gentamicin resistance cassette, mScarlet-I cassette, oriT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge