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pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta23

Plasmid

#106117

Purpose

Lentiviral expression of PRKRA delta23

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Lentiviral

Tags

DDK (FLAG) and myc

Mutation

AA 1-127

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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3TERM_bba_b0015_rev_chlo

Plasmid

#163931

Purpose

L0 part - terminator

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

from GenBank: KT364744.3, prokaryotic double terminator BBa_B0015

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pmCellFree_KA2

Plasmid

#145370

Purpose

Gateway destination vector for cell-free and mamalian expression with N-term 8xHis-tag and mCherry tag

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Type

Empty backbone

Tags

His and mCherry

Expression

Bacterial

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pMAD-Gate2

Plasmid

#25941

Depositor

Kamil Onder

Article

Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

Insert

Tags

VP16 Fusion Protein

Expression

Mammalian

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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p15aC-1D-5

Plasmid

#107865

Purpose

Bacterial expression of PIS and PI4P5K

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Inserts

PIS
phosphatidylinositol 4-phosphate 5-kinase

Tags

myc

Expression

Bacterial

Promoter

proD and proD (in operon)

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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plIVMD156

Plasmid

#160934

Purpose

In vivo mRNA display Gateway Destination construct

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop

Tags

HIS tag, 3'UTR MS2 Stem Loop

Expression

Yeast

Promoter

MET25

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pACYC-pVhb-vfFadBA

Plasmid

#215650

Purpose

pACYC Origin, CmR, with vfFadBA gene under Vhb promoter

Depositor

Brian Pfleger

Article

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Insert

thiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)

Expression

Bacterial

Promoter

Vhb

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWC

Plasmid

#112898

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mTFP1.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046879

Plasmid

#149238

Purpose

Bacterial expression plasmid for SARS-CoV-2 nsp2

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available Since

May 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCVD438

Plasmid

#63759

Purpose

eae gene (3476 bp) from pJY21 removed by SamI/SphI digestion, cloned into EcoRV/SphI sites of pACYC184. Insertional inactivation of tetracycline gene.

Depositor

James Kaper

Article

Jerse et al Proc Natl Acad Sci U S A. 1990 Oct;87(20

Insert

eae

Expression

Bacterial

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only