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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,380 results for: Cre

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Showing: 9421 - 9440 of 14380 results

  1. S1 1029 CP ABEMax strategy 1

    Plasmid
    #135357
    Purpose
    Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the N-terminus
    Depositor
    Alex Hewitt
    Publication
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABEMax-nSpyCas9 aa 1029
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    WT
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135357-plasmid-map-sequence-id-267707

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  2. 1029-AIDx-CP

    Plasmid
    #135341
    Purpose
    Expression of circular permutant nSpyCas9 at amino acid position 1029 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.
    Depositor
    Alex Hewitt
    Publication
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CP 1029 nSpyCas9-hAIDx
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    WT
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135341-plasmid-map-sequence-id-267692

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  5. ABE8.13-d

    Plasmid
    #136297
    Purpose
    expresses ABE8.13-d in mammalian cells
    Depositor
    Nicole Gaudelli
    Publication
    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABE8.13-d
    Tags
    C-terminal BPNLS
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available since
    March 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    136297-plasmid-map-sequence-id-269928

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  6. p3E-Clta

    Plasmid
    #109585
    Purpose
    Multisite gateway vector for 3' tagging with Clathrin light chain a
    Depositor
    Rob Parton
    Publication
    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Clathrin light chain a (clta Zebrafish)
    Use
    Zebrafish plasmids
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    109585-plasmid-map-sequence-id-222116

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  7. p3E-Dnm2a-DN

    Plasmid
    #109572
    Purpose
    Multisite gateway vector for 3' tagging with dominant negative Dynamin2a
    Depositor
    Rob Parton
    Publication
    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Dynamin2a (dnm2a Zebrafish)
    Use
    Zebrafish plasmids
    Mutation
    dominant negative
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    109572-plasmid-map-sequence-id-222102

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  8. p3E-Dnm2b-DN

    Plasmid
    #109574
    Purpose
    Multisite gateway vector for 3' tagging with dominant negative Dynamin2b
    Depositor
    Rob Parton
    Publication
    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Dynamin2b (dnm2b Zebrafish)
    Use
    Zebrafish plasmids
    Mutation
    dominant negative
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    109574-plasmid-map-sequence-id-222104

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  12. KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid
    #121819
    Purpose
    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121819-plasmid-map-sequence-id-237368

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  13. HD201: pMVP (L3-L2) IRES-eGFP + polyA

    Plasmid
    #121747
    Purpose
    pMVP L3-L2 entry plasmid, contains IRES2-eGFP+ polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of eGFP reporter from IRES sequence.
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IRES2-eGFP + polyA
    Use
    Gateway Cloning and Synthetic Biology
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121747-plasmid-map-sequence-id-237185

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  18. pOCC177

    Plasmid
    #118886
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal SNAP tag, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease and SNAP tag…
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118886-plasmid-map-sequence-id-232379

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  19. pUC19-F3QMY7

    Plasmid
    #103107
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    F3QMY7(Cas9 coding gene from Parasutterella excrementihominis YIT 11859)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103107-plasmid-map-sequence-id-201816

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Showing: 9421 - 9440 of 14380 results