178,448 results

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CROP-sgRNA-MS2

Plasmid

#153457

Purpose

CROP-seq vector with mCherry and x2 MS2 loops in gRNA scaffold

Depositor

Wolf Reik

Publication

Alda-Catalinas et al Cell Syst. 2020 Jul 22;11(1):25-41.e9. d

Insert

sgRNA empty backbone

Use

CRISPR

Expression

Mammalian

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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CARPID dCasRx-BASU

Plasmid

#153303

Purpose

express CARPID dCasRx-BASU fusion protein in mammalian cells

Depositor

Jian Yan, Liang Zhang

Publication

Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

Insert

dCasRx-BASU

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A in RfxCas13d

Promoter

EF-1a

Available since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pTetOne-mCherry-cNLS

Plasmid

#138527

Purpose

Inducible expression of mCherry with nuclear localization signal

Depositor

Christopher Grigsby, Ana Teixeira

Publication

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

mCherry

Tags

3x SV40 cNLS

Expression

Mammalian

Promoter

TRE3Gs

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-BFP

Plasmid

#138272

Purpose

Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor

Depositor

Xiao Wang

Publication

Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

Insert

BFP H66 PAM

Use

CRISPR

Expression

Mammalian

Mutation

H66, PAM motif

Promoter

Ef1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin

Plasmid

#134860

Purpose

Expresses EGFP-tagged recycling endosome marker

Depositor

David Andrews

Publication

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

RAB11B (RAB11B Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pQE-60NA-msGFP2

Plasmid

#135301

Purpose

Encodes a photostable monomeric superfolder GFP

Depositor

Benjamin Glick

Publication

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

msGFP2

Expression

Bacterial

Promoter

T5

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag

Plasmid

#132403

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mNeonGreen-Rab7

Plasmid

#129603

Purpose

In vivo visualization of Rab7 (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Rab7

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-hSyn-ASAP3Kv-WPRE

Plasmid

#132332

Purpose

expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

Depositor

Michael Lin

Publication

Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

Insert

ASAP3Kv

Use

AAV

Expression

Mammalian

Promoter

hSynapsin

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSET-mEosEM

Plasmid

#132708

Purpose

application in Correlative light-electron microscopy

Depositor

Pingyong Xu

Publication

Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

mEosEM

Expression

Bacterial

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDisplay-mgSrtA

Plasmid

#125792

Purpose

Transfected into cell of interest for membrane expression. The encoded mgSrtA could enable labeling of the interacting cells which is useful for recording cell-cell interactions.

Depositor

P. Chen

Publication

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

mgSrtA

Expression

Mammalian

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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fwYellow chromoprotein

Plasmid

#117841

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coli

Depositor

Anthony Forster

Publication

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, fwYellow

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Human DNA Binding Domain-Focused CRISPR Knockout Library

Pooled library

#123334

Purpose

Designed for genomic deletion screening of transcription factors by targeting the DNA binding domains.

Depositor

Christopher Vakoc

Publication

Huang et al Genes Dev. 2018 Jul 1;32(13-14):915-928.

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pQE60-RRF-His

Plasmid

#124132

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Ribosome recycling factor (frr Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-DDX3X

Plasmid

#116730

Purpose

Lentiviral expression of DDX3X

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

DDX3X (DDX3X Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mNeonGreen-2A-mTurquoise2

Plasmid

#98885

Purpose

Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

Depositor

Dorus Gadella

Publication

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mNeonGreen-2A-mTurquoise2

Expression

Mammalian

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-EF1a-fDIO-Cre

Plasmid

#121675

Purpose

Expresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)

Depositor

Esteban Engel, Alexander Nectow

Publication

Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

Has service

AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

Insert

NLS-CRE-HA

Use

AAV

Tags

3xHA and SV40-NLS

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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Synapsin-cyto-iATPSnFR1.0

Plasmid

#102556

Purpose

expresses cytoplasmic ATP sensor in neurons

Depositor

Baljit Khakh

Publication

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Use

AAV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMRE-Tn7-145

Plasmid

#118561

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn7 delivery vector

Expression

Bacterial

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTP288

Plasmid

#113460

Purpose

human Flag tagged Nbs1 in baculovirus transfer vector

Depositor

Tanya Paull

Publication

Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

Insert

Nbs1 (NBN Human)

Tags

Flag

Expression

Insect

Promoter

polyhedrin

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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