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pENTR D-TOPO-C3_38

Plasmid

#60279

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ACSL1 enhancer (ACSL1 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKT1_p.E17K

Plasmid

#82089

Purpose

Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT1 (AKT1 Human)

Use

Gateway entry vector

Mutation

E17K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_LIG1_WT_V5

Plasmid

#83006

Purpose

Gateway Donor vector containing LIG1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

LIG1 (LIG1 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FBXW7_WT

Plasmid

#81973

Purpose

Gateway Donor vector containing FBXW7, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXW7 (FBXW7 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CHUK_WT

Plasmid

#82083

Purpose

Gateway Donor vector containing CHUK , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CHUK (CHUK Human)

Use

Gateway entry vector

Mutation

V268I

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_WBSCR17_WT

Plasmid

#81837

Purpose

Gateway Donor vector containing WBSCR17 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

WBSCR17 (GALNT17 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FBXW7_p.R505C

Plasmid

#81507

Purpose

Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXW7 (FBXW7 Human)

Use

Gateway entry vector

Mutation

R505C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DAXX_WT_V5

Plasmid

#82934

Purpose

Gateway Donor vector containing DAXX, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

DAXX (DAXX Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HRAS_p.G12D

Plasmid

#81284

Purpose

Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HRAS (HRAS Human)

Use

Gateway entry vector

Mutation

G12D

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMARCB1_WT

Plasmid

#81743

Purpose

Gateway Donor vector containing SMARCB1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SMARCB1 (SMARCB1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-FLVCR1_STOP

Plasmid

#161058

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

FLVCR1 (FLVCR1 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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JDW 742 (pDONR221-EGFP-KRAS4B-G12V)

Plasmid

#156403

Purpose

Gateway middle entry clone encoding EGFP-fused to human KRAS4B with G12V mutation, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4B-G12V (KRAS Human)

Use

Gateway cloning

Tags

EGFP

Mutation

mutant KRAS4B-G12V

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_CXCR4_WT

Plasmid

#81957

Purpose

Gateway Donor vector containing CXCR4, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CXCR4 (CXCR4 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AURKB_WT_V5

Plasmid

#82963

Purpose

Gateway Donor vector containing AURKB, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

AURKB (AURKB Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_33

Plasmid

#60275

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

GLIS3 enhancer (GLIS3 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERBB2_WT

Plasmid

#82300

Purpose

Gateway Donor vector containing ERBB2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2 (ERBB2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AR_WT

Plasmid

#82094

Purpose

Gateway Donor vector containing AR , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AR (AR Human)

Use

Gateway entry vector

Mutation

p.81_82insQQ; deletion of AA397-482

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC52A3_STOP

Plasmid

#161347

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC52A3 (C20orf54 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_CBX1_WT

Plasmid

#82102

Purpose

Gateway Donor vector containing CBX1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CBX1 (CBX1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAPK3_WT

Plasmid

#82313

Purpose

Gateway Donor vector containing MAPK3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAPK3 (MAPK3 Human)

Use

Gateway entry vector

Mutation

L180X; p.135_136delSQ-insRPAAE; p.138_379del

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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