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pMIR-Report-Luc-KLF4-K5S-Mt344A

Plasmid

#34601

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Expression

Mammalian

Mutation

The KLF4 K5S region was mutated at the miR-344 bi…

Promoter

CMV

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-K5S-Mt206B

Plasmid

#34600

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Expression

Mammalian

Mutation

The KLF4 K5S region was mutated at the miR-206 bi…

Promoter

CMV

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
pENTR-E2F1-PIP3A

Plasmid

#37142

Depositor

Robert Duronio

Article

Shibutani et al Dev Cell. 2008 Dec;15(6):890-900.

Insert

E2F1-P!P3A (E2f1 Fly)

Use

Gateway

Mutation

I153, Y156, Y157 to Alanine

Available Since

July 9, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp4-a K>Q

Plasmid

#40044

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a K>Q (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Lysine 410,412,416,564,566,571, 572 to Glutamine

Promoter

CMV promoter

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-5-4a

Plasmid

#40045

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-5-4a (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Slp4 linker substituted by Slp5 linker domain (s…

Promoter

CMV promoter

Available Since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-a SHD

Plasmid

#40034

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a SHD (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

SHD domain (1-143)

Promoter

CMV promoter

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-N1 Btsz2

Plasmid

#40068

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Btsz2 (btsz Fly)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only
Topo_SpCas9.tracrRNA-CS1_AsCas12a DRv10

Plasmid

#237553

Purpose

TOPO vector for the cloning of the SpCas9 tracrRNA-CS1 - AsCas12a Direct Repeat (DR) v10 fragment into the pLCHKO_v4 hgRNA vector for scCHyMErA-Seq experiments.

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

SpCas9.tracrRNA-CS1_AsCas12a DRv10

Use

CRISPR

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pX461-ABE8e-nSpCas9-Intergenic sgRNA

Plasmid

#237463

Purpose

All-in-one base editor expressing adenine base editor (ABE8e-nSpCas9) and gRNA control targeting Intergenic site

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

Intergenic sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pX461-evoCDA1-nSpCas9-Intergenic sgRNA

Plasmid

#237464

Purpose

All-in-one base editor expressing cytosine base editor (evoCDA1-nSpCas9) and sgRNA control targeting Intergenic site

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

Intergenic sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pX462-ABE8e-nSaCas9-Intergenic sgRNA

Plasmid

#237465

Purpose

All-in-one base editor expressing adenine base editor (ABE8e-nSaCas9) and sgRNA control targeting Intergenic site

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

Intergenic sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pX461-ABE8e-nSpCas9-NRF1-ss-sgRNA

Plasmid

#237466

Purpose

All-in-one adenine base editor (ABE8e-nSpCas9) with gRNA targeting NRF1-Ex7 splice site

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

gRNA targeting NRF1-Ex7 splice site

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pX461-evoCDA1-nSpCas9-NRF1-ss-sgRNA

Plasmid

#237467

Purpose

All-in-one cytosine base editor (evoCDA1-nSpCas9) with sgRNA targeting NRF1-Ex7 splice site

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

sgRNA targeting NRF1-Ex7 splice site

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pX462-ABE8e-nSaCas9-NRF1-ss-sgRNA

Plasmid

#237468

Purpose

All-in-one adenine base editor (ABE8e-nSaCas9) with gRNA targeting NRF1-Ex7 splice site

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

gRNA targeting NRF1-Ex7 splice site

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF5-H4(A5,8,12,16)-HaloTag-FRT

Plasmid

#247455

Purpose

Expresses wild-type H4(A5,8,12,16)-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

H4(A5,8,12,16) has four mutations in the N-termin…

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1-H2B-mRFP1-pcdk1-EGFP1-FRT

Plasmid

#247334

Purpose

Expresses histone H2B fused with mRFP1, together with monomeric EGFP

Depositor

Kazuhiro Maeshima

Article

Hihara et al Cell Rep. 2012 Dec 27;2(6):1645-56. doi:

Insert

H2B (H2BC11) (H2BC11 Human, Synthetic)

Tags

mRFP1

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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PB-EF1-H4-HaloTag-IRES-Neo

Plasmid

#247450

Purpose

Expresses wild-type H4-Halo under EF1 promoter and can be randomly integrated by PiggyBac transposase

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-IB-H2B-PA-mCherry

Plasmid

#247339

Purpose

Expresses photoactivatable H2B-Cherry under CAG promoter and can be randomly integrated by PiggyBac transposase

Depositor

Kazuhiro Maeshima

Article

Nozaki et al Mol Cell. 2017 Jul 20;67(2):282-293.e7.

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

PAmCherry

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEF5-H4(Q5,8,12,16)-HaloTag-FRT

Plasmid

#247454

Purpose

Expresses wild-type H4(Q5,8,12,16)-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

H4(Q5,8,12,16) has four mutations in the N-termin…

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF5-H4Δ1-19-HaloTag-FRT

Plasmid

#247456

Purpose

Expresses wild-type H4(Δ1-19)-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

H4Δ1-19 lacks the initial 19 residues at the N-te…

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge