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pAAV-CAG-IFP2-P2A-GFP

Plasmid

#197161

Purpose

Expresses the protein of IFP2-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

IFP2-P2A-GFP

Use

AAV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mRhubarb720-P2A-GFP

Plasmid

#197159

Purpose

Expresses the protein of mRhubarb720-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mRhubarb720-P2A-GFP

Use

AAV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-BDFP1.6-P2A-GFP

Plasmid

#197154

Purpose

Expresses the protein of BDFP1.6-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

BDFP1.6-P2A-GFP

Use

AAV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mRhubarb719-P2A-GFP

Plasmid

#197152

Purpose

Expresses the protein of mRhubarb719-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mRhubarb719-P2A-GFP

Use

AAV

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGE010-Target1 (Mpphot)

Plasmid

#186728

Purpose

Binary vector for CRISPR/Cas9 (target 1: Mpphot [positive control]) in plants (for Agrobacterium-mediated genetic transformation).

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

Mphot

Expression

Bacterial

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pB2AR-miRFP718nano

Plasmid

#195751

Purpose

Beta-2-adrenergic receptor internally tagged with miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

B2AR-miRFP718nano

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGas-miRFP718nano

Plasmid

#195752

Purpose

G protein alpha-subunit internally tagged with miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

G protein alpha-s-miRFP718nano

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-mVenusC-14-HP1b Chromo

Plasmid

#191398

Purpose

HP1b chromo domain fused to C-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

FLAG-mVenusC-14-HP1b Chromo

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-NLS-MaSat ZF-18-mVenusN

Plasmid

#191392

Purpose

Zinc finger fused to N-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

Flag-NLS-MaSat ZF-18-mVenusN

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BLZinCh-P40

Plasmid

#188057

Purpose

Expression of BLZinCh-P40, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Article

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P40

Expression

Mammalian

Promoter

CMV promotor

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BLZinCh-P50

Plasmid

#188058

Purpose

Expression of BLZinCh-P50, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Article

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P50

Expression

Mammalian

Promoter

CMV promotor

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BLZinCh-P20

Plasmid

#188055

Purpose

Expression of BLZinCh-P20, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Article

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P20

Expression

Mammalian

Promoter

CMV promotor

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BLZinCh-P30

Plasmid

#188056

Purpose

Expression of BLZinCh-P30, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

Depositor

Maarten Merkx

Article

Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

Insert

BLZinCh-P30

Expression

Mammalian

Promoter

CMV promotor

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pmCherry2-CD86-mEYFP(Q69K)-mEGFP

Plasmid

#190751

Purpose

Expression of triple tagged CD86 with mCherry2, mEYFP(Q69K), and mEGFP in mammalian cells.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Insert

CD86 (CD86 Human)

Tags

mCherry2, mEGFP, and mEYFP(Q69K)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE010-Target2 (Mpphot)

Plasmid

#186729

Purpose

Binary vector for CRISPR/Cas9 (target 2: Mpphot [negative control]) in plants (for Agrobacterium-mediated genetic transformation).

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

Mphot

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p-preSufI(IAC)

Plasmid

#168516

Purpose

Modified form of pre-SufI in pET25b. With C-terminal HHHHHHC (6xHisC) extension and mutations C17I and C295A.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

pre-SufI(IAC)

Tags

6xHisC tag and HSV tag

Expression

Bacterial

Mutation

C17I and C295A

Promoter

T7

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-CBh.22

Plasmid

#183566

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 22

Expression

Mammalian

Promoter

CBh

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-EF1a.13

Plasmid

#183576

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 13

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCytERM-miRFP670nano3

Plasmid

#184674

Purpose

Signal anchor of rabbit cytochrome p450 and miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

CytERM-miRFP670nano3

Tags

miRFP670nano3

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-TetOn.7

Plasmid

#183581

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 7

Expression

Mammalian

Promoter

Tet-On

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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