We narrowed to 27,555 results for: STI;
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Plasmid#158137PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
ExpressionYeastMutationCloned in a TAG codon at the third serine residue…PromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSWD95Y14D
Plasmid#159098PurposeCckA FRET Sensor with cyclic-di-GMP point mutantDepositorInsertCckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691(Y514D), Linker SNVTRHRSAT, mRuby3
ExpressionBacterialMutationMutated Y514 to D514PromoterXyloseAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSWD95D293A
Plasmid#159099PurposeCckA FRET Sensor with DITE motif mutantDepositorInsertCckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691 (D293A), Linker SNVTRHRSAT, mRuby3
ExpressionBacterialMutationCckA D293 mutated to A293 and V199I on mRuby3 (P…PromoterXyloseAvailable SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMIII-Neo-mChe
Plasmid#153423PurposeRetroviral (MSCV) expression of mCherry fluorescent proteinDepositorInsertmCherry
UseRetroviralExpressionMammalianAvailable SinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFloat.assemble(AB).LC_v1
Plasmid#133083PurposeTier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. Low copy. Version 1.0.DepositorTypeEmpty backboneUseDestination vectorExpressionBacterialAvailable SinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFloat.assemble(AB).HC_v1
Plasmid#133084PurposeTier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. High copy. Version 1.0.DepositorTypeEmpty backboneUseDestination vectorExpressionBacterialAvailable SinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-RnBarr2
Plasmid#137798PurposeVisualization of beta-arrestin2DepositorAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-C3H2B
Plasmid#133387PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)DepositorTypeEmpty backboneExpressionMammalianPromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK0_028
Plasmid#123968PurposeEncodes the cROSA26 homology landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK systemDepositorInsertcRosa26 landing pad destination vector
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP1-N
Plasmid#128310PurposeExpress hNABP1 N-terminus (1–141 aa) in E. coli with a C-terminal His-tagDepositorInserthNABP1 N-terminal domain (OB fold, 1–141 aa) (NABP1 Human)
TagsHisExpressionBacterialMutationN-terminal domain (OB fold, 1–140 aa)PromoterT7Available SinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP2-C
Plasmid#128309PurposeExpress hNABP2 C-terminus(122–211 aa) in E. coli with a C-terminal His-tagDepositorInserthNABP2 C-terminal domain (122–211 aa) (NABP2 Human)
TagsHisExpressionBacterialMutationC-terminal domain (122–211 aa)PromoterT7Available SinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-4xUAS
Plasmid#125149PurposeVector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cellsDepositorInsert4 x upstream activating sequence (UAS)
UseStap-seq screening vectorAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
JL104: pMVP/Neo-DEST
Plasmid#121857PurposepMVP destination vector, empty expression vector backbone w/ Neo selection cassetteDepositorTypeEmpty backboneUsePmvp gateway destination vectorExpressionMammalianAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR_Trf2
Plasmid#125159PurposeGateway entry cloneDepositorInsertTrf2 (Trf2 Fly)
UseGateway entry vectorAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBDU-Atg17(F317D, I325A)
Plasmid#122275PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationPhenylalanine 317 to Aspartic acid; Isoleucine 32…Available SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBDU-Atg17(I325A)
Plasmid#122274PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationIsoleucine 325 to alanineAvailable SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(F317D, I325A)
Plasmid#122273PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationPhenylalanine 317 to Aspartic acid; Isoleucine 32…Available SinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(I325A)
Plasmid#122272PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationIsoleucine 325 to alanineAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(F317Q)
Plasmid#122271PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationPhenylalanine 317 to glutamineAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(F317D)
Plasmid#121391PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastMutationPhenylalanine 317 to Aspartic acidPromoterADH1 promoterAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only