We narrowed to 17,149 results for: tes

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pcDNA3.1(+)-MC38-TCRA-33-MC_hammer

Plasmid

#138989

Purpose

IVT template for the alpha subunit of the mouse TCR #33 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 CRY2-TSS-guide3

Plasmid

#125491

Purpose

CRISPR-mediated activation of CRY2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 CRY2-TSS-guide2

Plasmid

#125490

Purpose

CRISPR-mediated activation of CRY2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-H031gp160

Plasmid

#123272

Purpose

Mammalian expression plasmid for Env from the H031 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (H031) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-CH115gp160

Plasmid

#123280

Purpose

Mammalian expression plasmid for Env from the CH115 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (CH115) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-25711gp160

Plasmid

#111841

Purpose

Mammalian expression plasmid for Env from the 25711 HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (25711.2) Env

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-HF2-BE2

Plasmid

#120396

Purpose

Expresses HF2-BE2 in mammalian cells

Depositor

Junjiu Huang

Article

Liang et al Protein Cell. 2017 Aug;8(8):601-611. doi

Insert

HF2-BE2

Expression

Mammalian

Mutation

Mammalian codon-optimized Cas9-HF

Promoter

CMV

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a SVEM hphMX6 lys1-I-SceI cleavage site

Plasmid

#67648

Purpose

I-SceI cleavage site at lys1-hphMX6

Depositor

Kurt Runge

Article

Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

Inserts

lys1 (lys1 Fission Yeast)
hphMX6

Use

To test i-scei cleavage efficiency

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pF708-pET28a-Hs-delta38-METTL20-NHis wt

Plasmid

#85109

Purpose

expression of recombinant human mature (delta38) METTL20 wt with N-terminal 6xHis-tag

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human mature (delta38) METTL20 (METTL20 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgQKI-2201

Plasmid

#115449

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgQKI-2201 (QKI Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shMBNL3-0455

Plasmid

#115463

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shMBNL3-0455 (MBNL3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgQKI-2204

Plasmid

#115450

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgQKI-2204 (QKI Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shRBFOX1-3261

Plasmid

#115458

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shRBFOX1-3261 (RBFOX1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shRBFOX1-3260

Plasmid

#115457

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shRBFOX1-3260 (RBFOX1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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PGEX-6P1-hNF2-AR mutant

Plasmid

#107149

Purpose

bacterial expression

Depositor

Tina Izard

Article

Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

Insert

neurofibromin 2 (NF2 Human)

Tags

GST

Expression

Bacterial

Mutation

A585W-R588K

Promoter

Ptac

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-Fascin-Promoter (-402-0)

Plasmid

#89823

Purpose

Promoter luciferase assay

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Fascin1 (FSCN1 Human)

Use

Luciferase

Promoter

SV40

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3 N-t

Plasmid

#89980

Purpose

Expresses the GFP-tagged N-terminus (Josephin domain) of ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Mutation

construct contains aa 1-182; deleted aa 183-361

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3 *VBM

Plasmid

#89976

Purpose

Expresses GFP-tagged ataxin-3 with a mutation in the VCP-binding motif

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Mutation

mutated 282RKRR to 282HNHH

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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LacI/109-pHG165c

Plasmid

#90058

Purpose

WT LacI polymorphism with T at position 109

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LacI/109

Expression

Bacterial

Mutation

109

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLPCX2-HA-GATA3 C1(259-443)

Plasmid

#89832

Purpose

Express GATA3 C1 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Gata3 (Gata3 Mouse)

Use

Retroviral

Tags

HA

Mutation

C1 (AA 259-443)

Promoter

CMV

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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