We narrowed to 1,804 results for: FAST

1. pGREAT26

   Plasmid

   #170914

   Purpose

   GoldenBraid Double Transcriptional Unit - pEstradiol_Inducible_E-Luc_NtEUt+pNOS_Red-F_NOSt

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Plant

   Mutation

   c.375C>T silent mutation to remove BbsI site (…

   Promoter

   pEstradiol_Inducible

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Venus-7aa-ActA-pEGFP-C1

   Plasmid

   #177405

   Purpose

   To express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.

   Depositor

   David Andrews

   Article

   Osterlund et al Protocol Exchange (2022)

   Insert

   ActA tail anchor (actA Listeria monocytogenes)
   

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRiboI-APEX2m

   Plasmid

   #176843

   Purpose

   Expression of APEX2 from a codon-optimized gene (single-copy integrating plasmid)

   Depositor

   Jessica Seeliger

   Article

   Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

   Insert

   pRibo-APEX2m
   

   Use

   Integrating

   Expression

   Bacterial

   Promoter

   pRibo, theophilline riboswitch inducible hsp60 pr…

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCSN061

   Plasmid

   #101725

   Purpose

   Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.

   Depositor

   Rene Verwaal

   Article

   Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

   Inserts

   S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
   KanMX marker expression cassette.
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Promoter

   Heterologous TEF1 promoter from A. gossypii. and …

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUGW-RapACR_T111C-EYFP

   Plasmid

   #119195

   Purpose

   Fast anion channelrhodopsin mutant RapACR_T111C for time-resolved inhibition of neuronal spiking

   Depositor

   John Spudich

   Article

   Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

   Insert

   Anion channelrhodopsin RapACR fast mutant T111C
   

   Use

   Lentiviral

   Tags

   EYFP

   Mutation

   changed threonine 111 to cysteine

   Promoter

   Ubiquitin

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRibo-APEX2m

   Plasmid

   #176842

   Purpose

   Expression of APEX2 from a codon-optimized gene (multi-copy episomal plasmid)

   Depositor

   Jessica Seeliger

   Article

   Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

   Insert

   pRibo-APEX2m
   

   Expression

   Bacterial

   Promoter

   pRibo, theophilline riboswitch inducible hsp60 pr…

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGP-CMV-NES-fRGECO1

   Plasmid

   #125247

   Purpose

   Expresses red fluorescent calcium sensor in mammalian cells

   Depositor

   Katalin Torok

   Article

   Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

   Insert

   fRGECO2
   

   Tags

   NES signal

   Expression

   Mammalian

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGREAT18

   Plasmid

   #170906

   Purpose

   GoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Plant

   Mutation

   c.375C>T silent mutation to remove BbsI site (…

   Promoter

   pAtUBQ10

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJ414-PCY1

   Plasmid

   #98150

   Purpose

   Expresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZA1

    Plasmid

    #158479

    Purpose

    Entry clone for Golden Gate assembly. Golden Gate compatible wild-type A. thaliana ZAR1 with STOP codon

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1 (ZAR1 Mustard Weed)
    

    Use

    Golden gate entry vector

    Mutation

    STOP codon

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDx_mScarlet-I-SYFP2

    Plasmid

    #189761

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet-I-SYFP2 fusion protein for FRET analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet-I-SYFP2
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGREAT17

    Plasmid

    #170905

    Purpose

    GoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    p35SoTMV

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGREAT4

    Plasmid

    #170892

    Purpose

    GoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NOSt+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    pG10-90

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmEos2-Linker_PCNA-MutC

    Plasmid

    #98272

    Purpose

    for low level expression of mEos2-PCNA in mammalian cells

    Depositor

    Mike Heilemann

    Article

    Zessin et al Sci Rep. 2016 Jan 13;6:18779. doi: 10.10

    Insert

    PCNA (PCNA Human)
    

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Silent mutated Sequence: GACGCCGTAGTTATATCTTGC (O…

    Promoter

    CMV

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNB785

    Plasmid

    #60164

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus-PEST
    

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    SIC1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNB787

    Plasmid

    #60152

    Purpose

    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus-PEST
    

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    LEU1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNB789

    Plasmid

    #60166

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus-PEST
    

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    RNR1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDx_FusionRed-SYFP2

    Plasmid

    #189760

    Purpose

    Dual expression vector for bacteria and mammalian cells producing FusionRed-SYFP2 fusion protein for FRET analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    FusionRed-SYFP2
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDx_mScarlet-SYFP2

    Plasmid

    #189762

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet-SYFP2 fusion protein for FRET analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet-SYFP2
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABR Bcd-LEXY

    Plasmid

    #182594

    Purpose

    Drosophila integration plasmid expressing Bcd-LEXY fusion protein under the maternal tubulin promoter.

    Depositor

    Jared Toettcher

    Article

    Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

    Insert

    Bicoid (bcd Fly)
    

    Tags

    LEXY

    Expression

    Insect

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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