We narrowed to 6,877 results for: GFP expression plasmids

1. pAc5.1B-EGFP-DmPABPC1-V5His6_G

   Plasmid

   #146352

   Purpose

   Insect Expression of DmPABPC1

   Depositor

   Elisa Izaurralde

   Insert

   DmPABPC1 (pAbp Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one non silent mutation G26S, and seven silent mu…

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mCherry-7aa-cb5-pEGFP-C1

   Plasmid

   #177407

   Purpose

   To express mCherry fused to endoplasmic reticulum (ER) targeting sequence "Cb5". mCherry faces the cytosol.

   Depositor

   David Andrews

   Insert

   Cb5 tail anchor (Cyb5r4 Rat)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mCerulean3-Bcl2-ActA-pEGFP-C1

   Plasmid

   #177418

   Purpose

   To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).

   Depositor

   David Andrews

   Insert

   Bcl-2-ActA, Bcl2-acta, Bcl-acta
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mCerulean3-Bcl2-cb5-pEGFP-C1

   Plasmid

   #177421

   Purpose

   Express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).

   Depositor

   David Andrews

   Insert

   Bcl-2-Cb5, Bcl2-cb5, Bcl-cb5
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mT2A-sfGFP-linker-iCre-ERT2-FNF

   Plasmid

   #182341

   Purpose

   Recombineering cassette plasmid for T2A-sfGFP-linker-iCre-ERT2-FNF

   Depositor

   Mario Capecchi

   Insert

   T2A-sfGFP-linker-iCre-ERT2-FNF
   

   Use

   Mouse Targeting; Flp / frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA

   Plasmid

   #105648

   Purpose

   Expression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeks

   Depositor

   Sandra Rieger

   Insert

   Tol2-Isl2b:GFP:polyA (isl2b Zebrafish)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Pten_shRNAmir

   Plasmid

   #178044

   Purpose

   CAG tdGFP Pten shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

   Depositor

   Mario Capecchi

   Insert

   Pten_1523 shRNAmir
   

   Use

   Cre/Lox and RNAi; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1B-EGFP-DmNot3-mut2-dsRNAres_Q

   Plasmid

   #147264

   Purpose

   Insect Expression of DmNot3-mut2-dsRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmNot3-mut2-dsRNAres (Not3 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation compared to the sequence give…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAc5.1B-EGFP-DmNot3-F764A-dsRNAres_Q

   Plasmid

   #147261

   Purpose

   Insect Expression of DmNot3-F764A-dsRNAres

   Depositor

   Elisa Izaurralde

   Insert

   DmNot3-F764A-dsRNAres (Not3 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation compared to the sequence give…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAc5.1B-EGFP-DmNot3-mut1-dsRNAres_Q

    Plasmid

    #147263

    Purpose

    Insect Expression of DmNot3-mut1-dsRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmNot3-mut1-dsRNAres (Not3 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAc5.1B-EGFP-DmNot3-F818A-dsRNAres_Q

    Plasmid

    #147262

    Purpose

    Insect Expression of DmNot3-F818A-dsRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmNot3-F818A-dsRNAres (Not3 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Renilla_shRNAmir

    Plasmid

    #178046

    Purpose

    CAG tdGFP Renilla shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Renilla_713 shRNAmir
    

    Use

    Cre/Lox and RNAi; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Luciferase_shRNAmir

    Plasmid

    #178045

    Purpose

    CAG tdGFP Luciferase shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Luciferase_1309 shRNAmir
    

    Use

    Cre/Lox and RNAi; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p667-UBC-GFP-V5_IDG-K

    Plasmid

    #135242

    Purpose

    Gateway destination clone of GFP (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    GFP-V5
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHAGE-CMV-Flag-GFP_IDG-K

    Plasmid

    #135275

    Purpose

    Gateway destination clone of GFP (as control) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

    Depositor

    Ben Major

    Insert

    Flag-GFP
    

    Use

    Lentiviral; Gateway destination

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. H2B-sfGFP-T2A-iCre

    Plasmid

    #178546

    Purpose

    pCMV-Histone H2B-sfGFP-T2A-iCre-bpA-FRT-Neo*-FRT

    Depositor

    Mario Capecchi

    Insert

    Histone H2B-sfGFP-T2A-iCre
    

    Use

    Cre/Lox and Mouse Targeting; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHBS1051 TDP43 RRM-GFP F-Y

    Plasmid

    #107802

    Purpose

    To test the effect of sequence on TDP43 droplet properties

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All phenylalanine to tyrosine in LLPS reporter

    Promoter

    Available since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHBS932 TDP43 RRM-GFP Φ-S

    Plasmid

    #107798

    Purpose

    To test the effect of sequence on TDP43 droplet properties

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All hydrophobics to serine

    Promoter

    Available since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIRES-GFP-hFJX1-myc

    Plasmid

    #46928

    Purpose

    coexpression of FJX1-myc and GFP in mammalian cells

    Depositor

    Natasha Deane, R.D. Beauchamp

    Insert

    FJX1 (FJX1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pT2/shαSMA/GFP4_Seq1/clone1

    Plasmid

    #206138

    Purpose

    Knockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Insert

    alpha-SMA (Acta2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge