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pc3XB-ZF104

Plasmid

#13238

Depositor

Daniel Voytas

Publication

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF104

Expression

Mammalian

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

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pc3XB-ZF105

Plasmid

#13239

Depositor

Daniel Voytas

Publication

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF105

Expression

Mammalian

Available since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-Poc5

Plasmid

#261520

Purpose

N-terminal GFP-tagged mouse POC5

Depositor

Aimin Liu

Publication

Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

Insert

Poc5 (Poc5 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 14, 2026

Availability

Academic Institutions and Nonprofits only

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Upstream U6 24-mer acceptor backbone

Plasmid

#257852

Purpose

For cloning of next-generation 24-mer pegArrays, with upstream U6

Depositor

Jonathan Weissman

Publication

Colgan et al Cell. 2026 Oct 8:S0092-8674(26)01196-7.

Type

Empty backbone

Expression

Mammalian

Available since

Oct. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGGS-HHIP

Plasmid

#261261

Purpose

Expresses mouse HHIP under CAG promoter.

Depositor

Cliff Tabin

Publication

Yan et al Nat Commun. 2025 Nov 27;16(1):10643. doi

Insert

Hedgehog-Interacting Protein (Hhip Mouse)

Expression

Mammalian

Available since

Oct. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pLIB-TSP-UL32

Plasmid

#250566

Purpose

Insect cell expression of HSV-1 UL32 with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Allison Didychuk

Publication

Bailey et al PLoS Pathog. 2026 Sep 10;22(9):e1014540.

Insert

UL32

Tags

TwinStrepTagII-HRV3C

Expression

Insect

Promoter

polyhedrin

Available since

Oct. 1, 2026

Availability

Academic Institutions and Nonprofits only

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pLIB-TSP-UL52

Plasmid

#250567

Purpose

Insect cell expression of HCMV UL52 with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Allison Didychuk

Publication

Bailey et al PLoS Pathog. 2026 Sep 10;22(9):e1014540.

Insert

UL52

Tags

TwinStrepTagII-HRV3C

Expression

Insect

Promoter

polyhedrin

Available since

Oct. 1, 2026

Availability

Academic Institutions and Nonprofits only

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pBAD-cysJ(hisJ)

Plasmid

#261397

Purpose

Expresses N-terminally his-tagged sulfite reductase flavoprotein (cysJ(hisJ))

Depositor

M. Elizabeth Stroupe

Publication

Askenasy et al J Biol Chem. 2015 Jul 31;290(31):19319-3

Insert

cysJ

Tags

6xhis on SiRFP

Expression

Bacterial

Available since

Aug. 31, 2026

Availability

Academic Institutions and Nonprofits only

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Destination vector

Plasmid

#254019

Purpose

It serves as a destination vector in the EffiModular process.

Depositor

Dae-Hee Lee

Publication

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

sfGFP

Use

Synthetic Biology

Available since

June 17, 2026

Availability

Academic Institutions and Nonprofits only

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pN1_NUP62-Fv_EGFP3

Plasmid

#252749

Purpose

Expression of NUP62 with three tandem EGFPs. FV is a modified FKBP domain (aka DmrB) whose dimerization is induced by addition of Rapalog2. Blocks nulcear pore upon addition of Rapalog2

Depositor

Kristen Verhey

Publication

Takao et al Curr Biol. 2014 Oct 6;24(19):2288-94. do

Insert

NUP62 (Nup62 Mouse)

Tags

FV, 3xEGFP

Expression

Mammalian

Available since

April 3, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pN1_NUP62-EGFP3

Plasmid

#252748

Purpose

Expression of NUP62 with three tandem EGFPs.

Depositor

Kristen Verhey

Publication

Takao et al Curr Biol. 2014 Oct 6;24(19):2288-94. do

Insert

NUP62 (Nup62 Mouse)

Tags

E3xGFP

Expression

Mammalian

Available since

April 3, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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hVAMP3(1-77) WT

Plasmid

#206028

Purpose

Expresses the SNARE motif of wildtype human VAMP3 in bacteria

Depositor

Geert van den Bogaart

Publication

Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

Insert

Vesicle-associated membrane protein 3 (VAMP3 Human)

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-FGD3

Plasmid

#242832

Purpose

Encodes human FGD3 and N-terminal EGFP tag

Depositor

Cheng-han Yu

Publication

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

FGD3 (FGD3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

N/A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-EBFP2-VAV1

Plasmid

#242837

Purpose

Encodes human VAV1 and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Publication

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

VAV1 (VAV1 Human)

Use

Lentiviral

Tags

EBFP2

Expression

Mammalian

Mutation

N/A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET50del_SynPspA_R44K/E126Q/E179Q

Plasmid

#223819

Purpose

Bacterial expression of SynPspA R44K/E126Q/E179Q with N-terminal 6xHis-Tag and 3C cleavage site

Depositor

Carsten Sachse

Publication

Junglas et al Nat Struct Mol Biol. 2025 Jan;32(1):23-3

Insert

SynPspA

Tags

6xHis

Expression

Bacterial

Mutation

R44K, E126Q, E179Q

Available since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pX330 - LRR1 Terminal CRISPR

Plasmid

#221705

Purpose

Cas9 targeting plasmid with gRNA specific for LRR1 N-terminus

Depositor

Aga Gambus

Publication

Jones et al iScience. 2024 Jun 12;27(7):110260. doi:

Insert

TGTAGCTTCATCTCGCCCAA

Use

CRISPR

Promoter

Chicken Beta-actin

Available since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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POC1426

Plasmid

#221573

Purpose

Plasmid containing the Insert 1 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1427

Plasmid

#221574

Purpose

Plasmid containing the Insert 2 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1428

Plasmid

#221575

Purpose

Plasmid containing the Insert 3 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1429

Plasmid

#221576

Purpose

Plasmid containing the Insert 4 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge