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pBABE (hygro) mEOS2 hYAP 1-2delta

Plasmid

#124162

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

mEOS2-YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_MTM1_p.D298E

Plasmid

#81711

Purpose

Gateway Donor vector containing MTM1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MTM1 (MTM1 Human)

Use

Gateway entry vector

Mutation

D298E; E410K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23 A82V/T230A/D637G 2014 EBOV Delta-Mucin-Like-Domain

Plasmid

#86026

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Lacks mucin-like domain; Changed alanine 82 to va…

Promoter

CMV IE

Available Since

July 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_SLC46A1_p.W299S

Plasmid

#81622

Purpose

Gateway Donor vector containing SLC46A1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SLC46A1 (SLC46A1 Human)

Use

Gateway entry vector

Mutation

W299S; Q429P

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TBL1XR1_p.R166Q

Plasmid

#81483

Purpose

Gateway Donor vector containing TBL1XR1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TBL1XR1 (TBL1XR1 Human)

Use

Gateway entry vector

Mutation

R166Q; L512P

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CG_p.K973R

Plasmid

#81628

Purpose

Gateway Donor vector containing PIK3CG , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CG (PIK3CG Human)

Use

Gateway entry vector

Mutation

E2G; K973R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CG_p.W212C

Plasmid

#81630

Purpose

Gateway Donor vector containing PIK3CG , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CG (PIK3CG Human)

Use

Gateway entry vector

Mutation

E2G; W212C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CG_p.V168M

Plasmid

#81542

Purpose

Gateway Donor vector containing PIK3CG , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CG (PIK3CG Human)

Use

Gateway entry vector

Mutation

E2G; V168M

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPIAL4G_WT

Plasmid

#81900

Purpose

Gateway Donor vector containing PPIAL4G , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PPIAL4G (PPIAL4G Human)

Use

Gateway entry vector

Mutation

I6V; Q24L; H69R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAG2_p.E134D

Plasmid

#81629

Purpose

Gateway Donor vector containing STAG2 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAG2 (STAG2 Human)

Use

Gateway entry vector

Mutation

E134D

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAG2_p.P572S

Plasmid

#81598

Purpose

Gateway Donor vector containing STAG2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAG2 (STAG2 Human)

Use

Gateway entry vector

Mutation

P572S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAG2_p.L997W

Plasmid

#81588

Purpose

Gateway Donor vector containing STAG2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAG2 (STAG2 Human)

Use

Gateway entry vector

Mutation

L997W

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAG2_p.R252Q

Plasmid

#81589

Purpose

Gateway Donor vector containing STAG2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAG2 (STAG2 Human)

Use

Gateway entry vector

Mutation

R252Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAG2_p.R541K

Plasmid

#81578

Purpose

Gateway Donor vector containing STAG2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAG2 (STAG2 Human)

Use

Gateway entry vector

Mutation

R541K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3IP1_WT

Plasmid

#81853

Purpose

Gateway Donor vector containing PIK3IP1 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3IP1 (PIK3IP1 Human)

Use

Gateway entry vector

Mutation

T251S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3IP1_p.G185E

Plasmid

#81339

Purpose

Gateway Donor vector containing PIK3IP1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3IP1 (PIK3IP1 Human)

Use

Gateway entry vector

Mutation

G185E; T251S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Stx3 triple mutant (F31A D33A E34A)

Plasmid

#86945

Purpose

Expresses human Stx3 with triple mutation in the apical targeting signal (F31A D33A E34A)

Depositor

Thomas Weimbs

Article

Soo Hoo et al PLoS One. 2016 Sep 23;11(9):e0163671. do

Insert

STX3 triple mutant (F31A D33A E34A) (STX3 Human)

Tags

myc-His6

Expression

Mammalian

Mutation

triple mutant (F31A D33A E34A)

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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TRE-dCas9(sa)-VP64-AAV

Plasmid

#213035

Purpose

dCas9-VP64 expressed under control of TRE

Depositor

Nadav Ahituv

Article

Nguyen et al Nat Biotechnol. 2025 Feb 4:10.1038/s4158

Insert

dCas9

Use

AAV and CRISPR

Tags

NLS and NLS-VP64

Promoter

TRE, miniCMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLL5.0 shCavin1KDR-EGFP

Plasmid

#187254

Purpose

Lentiviral expression of shRNA targeting Cavin1 + reconstitution of mouse Cavin1 (Cavin1 rescue)

Depositor

Alpha Yap

Article

Teo et al Dev Cell. 2020 Jul 6;54(1):75-91.e7. doi

Insert

ShRNA for Cavin1 (Cavin1 Mouse)

Use

Lentiviral

Tags

EGFP

Promoter

U6, 5'LTR

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-SAM-Puro

Plasmid

#198713

Purpose

For expression of guide RNAs and the SAM transcriptional activator for CRISPRa in mammalian cells.

Depositor

Tyler Jacks

Article

Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

Inserts

U6-MS2-gRNAscaffold
EF1a-MPH-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a and U6

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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