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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,380 results for: Cre

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Showing: 9621 - 9640 of 14380 results

  5. Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G

    Plasmid
    #157984
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Publication
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (tRNA-Tail-8A8G)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    157984-plasmid-map-sequence-id-355759

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  8. EGFP-uTEV1-MAVS-mTA

    Plasmid
    #170999
    Purpose
    HiLITR protease with MAVS [R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)
    Depositor
    Alice Ting
    Publication
    Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:
    Insert
    EGFP-uTEV1-MAVS(tmd)[R537A]
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Mutation
    MAVS(tmd)[R537A]
    Promoter
    pTRE-tight (TetOn)
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    170999-plasmid-map-sequence-id-335667

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  9. EGFP-uTEV1-MAVS-mTA4

    Plasmid
    #171002
    Purpose
    HiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)
    Depositor
    Alice Ting
    Publication
    Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:
    Insert
    EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Mutation
    MAVS(tmd)[Y536delinsFIVLI]
    Promoter
    pTRE-tight (TetOn)
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    171002-plasmid-map-sequence-id-335829

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  12. B-SpCas9

    Plasmid
    #126760
    Purpose
    Expression plasmid for human codon-optimized Blackjack-SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of WT SpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    amino acids 1005-1013 replaced with two glycine
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126760-plasmid-map-sequence-id-261519

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  13. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA- Pconstitutive-sgRNA(Sth3)_blaA

    Plasmid
    #133347
    Purpose
    expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets cpaA and second one targets blaA (from Caulobacter crescentus)
    Depositor
    Michael Laub
    Publication
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA_cpaA and sgRNA_blaA
    Use
    CRISPR
    Promoter
    constitutive
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133347-plasmid-map-sequence-id-269540

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  15. Smp_090100-Cd4-bio-His

    Plasmid
    #120655
    Purpose
    Expression of recombinant S. mansoni protein
    Depositor
    Gavin Wright
    Publication
    Cécile et al bioRXiv
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Smp_090100 (Smp_090100 Schistosoma mansoni)
    Tags
    ratCd4(d3+4)-bio-6His
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    120655-plasmid-map-sequence-id-234477

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Showing: 9621 - 9640 of 14380 results