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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 9621 - 9640 of 23077 results

  1. pBN083

    Plasmid
    #173445
    Purpose
    express E. coli E69 (O9a:K30) Wzc_K540M with Y705E, Y706E
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc_K540M with Y705E, Y706E
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M, Y705E, Y706E
    Promoter
    araBAD
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    173445-plasmid-map-sequence-id-344777

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  4. pBN008

    Plasmid
    #167949
    Purpose
    express E. coli E69 (O9a:K30) Wzc with K540M mutation
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30), K540M nutant
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M
    Promoter
    araBAD
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167949-plasmid-map-sequence-id-344798

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  5. pBN093

    Plasmid
    #167951
    Purpose
    express E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M, delete motif 3
    Promoter
    araBAD
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167951-plasmid-map-sequence-id-344776

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  8. pTJV1ScGm-rpoB

    Plasmid
    #166979
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aacC1 for gentamycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Insert
    gentamycin-3-acetyltransferase (aac(3)-Ia )
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Available Since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166979-plasmid-map-sequence-id-331330

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  9. pET11a-Z-N-DiB2

    Plasmid
    #168473
    Purpose
    N-fragment of the DiB2‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain DiB2‐split-Zip protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10
    Insert
    N-fragment of DiB2 + Leucine Zipper
    Tags
    His-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168473-plasmid-map-sequence-id-332731

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Showing: 9621 - 9640 of 23077 results