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LPUtopia-7_CoV-2

Plasmid

#224782

Purpose

Landing Pad donor vector containing partial RdRp and N gene sequences from SARS-CoV-2 for fluorescent reporter assay

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

partial RdRp and N gene (N, ORF1ab SARS-CoV-2)

Expression

Mammalian

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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p40651-EFSNS-FMR1_e1-24xms2

Plasmid

#222968

Purpose

MS2 mRNA reporter with intact FMR1 5' UTR and exon 1 sequence

Depositor

Xinyu Zhao

Article

Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

Insert

FMR1-31CGG 5' UTR and exon1

Expression

Mammalian

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-99a-WT

Plasmid

#215880

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-99a-WT (MIR99A Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSG071

Plasmid

#214257

Purpose

Hybrid PaFtsH1NFtsH2 protease: PaftsH2SD -PaftsH21-462-PaftsH1454-1920

Depositor

Ute Romling

Article

Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

Insert

Hybrid PaFtsH protease PaFtsH1NFtsH2 of Pseudomonas aeruginosa SG17M

Expression

Bacterial

Mutation

Hybrid PaFtsH2NFtsH1 protease: First 18 nucleotid…

Promoter

araBAD promoter

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSG075

Plasmid

#214258

Purpose

Hybrid PaFtsH2NFtsH1 protease: PaftsH2SD -PaftsH11-453-PaftsH2463-1881 in pJN105

Depositor

Ute Romling

Article

Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

Insert

Hybrid PaFtsH protease PaFtsH2NFtsH1 of Pseudomonas aeruginosa SG17M

Expression

Bacterial

Mutation

Hybrid PaFtsH2NFtsH1 protease: First 18 nucleotid…

Promoter

araBAD promoter

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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AA081

Plasmid

#215977

Purpose

Fragmid fragment: (N' terminus) HIV Nuclear Export Sequence

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; HIV-NES_v1.2; HIV-NES_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSB55 - pL0_CrGLK (CDS)

Plasmid

#203903

Purpose

MoClo compatible L0 plasmid containing the CrGLK coding sequence

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CrGLK

Expression

Plant

Mutation

Silent mutation of BpiI or BsaI cut site for MoCl…

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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TLNRD1_35-345_eGFP

Plasmid

#208881

Purpose

Express eGFP-TLNRD1 lacking N and Cterm domains (TLNRD1 amino acids 35-345) in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

TLNRD1 (TLNRD1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

amino acid sequence 35-345

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pUC57-RPLP15UTR-hRluc-A60

Plasmid

#182639

Purpose

Transcription template plasmid for RPLP1 5'UTR followed by Renilla luciferase open reading frame and polyA sequence

Depositor

Stephen Floor

Article

Calviello et al Nucleic Acids Res. 2021 May 21;49(9):533

Insert

Renilla luciferase

Use

Luciferase

Expression

Mammalian

Mutation

The transcription is driven by a T7 promoter, pai…

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T-F41DW42D_AB

Plasmid

#148372

Purpose

Insect Expression of Dm4E-T-F41DW42D

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Dm4E-T-F41DW42D (4E-T Fly)

Expression

Insect

Mutation

5 non silent mutation L127S, E493G, H628R, N659S,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPAN2-del246-249-dsRNAres_Y

Plasmid

#148065

Purpose

Insect Expression of DmPAN2-del246-249-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-del246-249-dsRNAres (PAN2 Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmPAN2-del246-249-dsRNAres_Y

Plasmid

#148034

Purpose

Insect Expression of DmPAN2-del246-249-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-del246-249-dsRNAres (PAN2 Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-H19A_V

Plasmid

#147799

Purpose

Mammalian Expression of HsDCP1a-H19A

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP1a-H19A (DCP1A Human)

Expression

Mammalian

Mutation

two silent mutations T237C and T1716G compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-N74G_V

Plasmid

#147800

Purpose

Mammalian Expression of HsDCP1a-N74G

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP1a-N74G (DCP1A Human)

Expression

Mammalian

Mutation

two silent mutations T237C and T1716G compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-Q18AH19A_V

Plasmid

#147801

Purpose

Mammalian Expression of HsDCP1a-Q18AH19A

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP1a-Q18AH19A (DCP1A Human)

Expression

Mammalian

Mutation

two silent mutations T237C and T1716G compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-R72G_V

Plasmid

#147802

Purpose

Mammalian Expression of HsDCP1a-R72G

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP1a-R72G (DCP1A Human)

Expression

Mammalian

Mutation

two silent mutations T237C and T1716G compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-R72GN74G_V

Plasmid

#147803

Purpose

Mammalian Expression of HsDCP1a-R72GN74G

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP1a-R72GN74G (DCP1A Human)

Expression

Mammalian

Mutation

two silent mutations T237C and T1716G compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsDCP2_1-244_V

Plasmid

#147790

Purpose

Mammalian Expression of HsDCP2_1-244

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP2_1-244 (DCP2 Human)

Expression

Mammalian

Mutation

one silent mutation A429G compared to the sequenc…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot3-F764E-dsRNAres_U

Plasmid

#147721

Purpose

Insect Expression of DmNot3-F764E-dsRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot3-F764E-dsRNAres (Not3 Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpH-HsNot3_607-753_U

Plasmid

#147724

Purpose

Bacterial Expression of HsNot3_607-753

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3_607-753 (CNOT3 Human)

Expression

Bacterial

Mutation

two silent mutations compared to the sequence giv…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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