We narrowed to 19,856 results for: IRE;

Showing: 9641 - 9660 of 19856 results

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C1A-C1A-EYFP

Plasmid

#61155

Purpose

Diacylglycerol (DAG) reporter

Depositor

Tobias Meyer

Article

Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

Insert

C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)

Tags

EYFP

Expression

Mammalian

Mutation

C1A from PKCG amino acids 26-89

Promoter

CMV

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.tRFP

Plasmid

#57823

Purpose

3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP

Use

CRISPR and Lentiviral

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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ERK-KTR-mStrawberry reporter

Plasmid

#158686

Purpose

This plasmid is a ERK pathway reporter. It has CMV promoter driving the expression of ERK-KTR fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-ATG8(416)/GFP-AUT7(416)

Plasmid

#49425

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

Depositor

Daniel Klionsky

Article

Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Tags

GFP

Expression

Bacterial and Yeast

Promoter

ATG8

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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VCP(wt)-EGFP

Plasmid

#23971

Depositor

Nico Dantuma

Article

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Insert

Valosin containing protein (VCP Human)

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

none

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2

Plasmid

#221712

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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AKT-translocation-mStrawberry reporter

Plasmid

#158685

Purpose

This plasmid is a AKT pathway reporter. It has 1EF1a promoter driving the expression of truncated FoxO1 fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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Nrf1-Myc pcDNA3.1(+)-C-Myc

Plasmid

#169125

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hNrf1 (NFE2L1 Human)

Expression

Mammalian

Mutation

wt

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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8522-M01-663

Plasmid

#225660

Purpose

Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (green configuration)

Depositor

Dominic Esposito

Article

Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

Insert

dsCopGFP

Use

Lentiviral

Promoter

SORE6-mCMVp

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AY22_pgRNA.R5.1

Plasmid

#100294

Purpose

CCR5-targeting gRNA expression plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

gRNA spacer

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6 RNA Pol III promoter human snRNA

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pMRXIP TMEM41B-GFP

Plasmid

#117414

Purpose

Expresses TMEM41B tagged with GFP in mammalian cells

Depositor

Noboru Mizushima

Article

Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

Insert

TMEM41B (TMEM41B Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pHTNW

Plasmid

#136403

Purpose

Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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HotPETase_pET21b

Plasmid

#215407

Purpose

Expression construct for HotPETase gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

HotPETase

Expression

Bacterial

Mutation

S121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKM512

Plasmid

#140192

Purpose

For curing of Orbit-integrated plasmids and associated drug marker

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Inserts

zeocin resistance gene
Bxb1gp47 (directionality factor)

Expression

Bacterial

Mutation

Cloned in gp47 directinality factor in place of R…

Promoter

EM7 and Ptet

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry

Plasmid

#159655

Purpose

Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Type

Empty backbone

Use

CRISPR

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-ATG5

Plasmid

#99573

Purpose

Expresses Cas9 with sgRNA targeting Exon 7 of ATG5

Depositor

Edward Campbell

Article

Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

Insert

gRNA targeting ATG5 (ATG5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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p38-KTR-mStrawberry reporter

Plasmid

#158687

Purpose

This plasmid is a p38 pathway reporter. It has CMV promoter driving the expression of p38-KTR fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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RAR-TRE-mStrawberry reporter

Plasmid

#158683

Purpose

This plasmid is a RAR pathway reporter. It has a Retinoic Acid Receptor Response Element promoter driving the expression of mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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mApple-M1 Spastin

Plasmid

#134461

Purpose

M1 Spastin expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

M1 Spastin (SPAST Human)

Expression

Mammalian

Mutation

Met to Ala change at amino acid 87

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCW57-RFP-P2A-MCS (Neo)

Plasmid

#89182

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Article

Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Promoter

TRE

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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