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Klf5-eGFP-PGK-H2BRFP

Plasmid

#241865

Purpose

Klf5 epicenter-driven fluorescent reporter

Depositor

Elaine Fuchs

Article

Ge et al Cell. 2017 May 4;169(4):636-650.e14. doi

Insert

Klf5-eGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr 201_PHIP

Plasmid

#240328

Purpose

Entry vector for Gateway with PHIP

Depositor

Junjie Chen

Article

Nie et al Genes Dev. 2022 Sep 1;36(17-18):1016-103

Insert

PHIP (PHIP Human)

Expression

Bacterial

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr201_TNKS

Plasmid

#240329

Purpose

Entry vector for Gateway with TNKS

Depositor

Junjie Chen

Article

Nie et al Mol Cell Proteomics. 2020 Dec;19(12):201

Insert

TNKS (TNKS Human)

Expression

Bacterial

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pET50del_SynPspA_H1-5

Plasmid

#223820

Purpose

Bacterial expression of SynPspA H1-5 with N-terminal 6xHis-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Jan;32(1):23-3

Insert

SynPspA

Tags

6xHis

Expression

Bacterial

Mutation

truncation of helix 0

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET50delCT_Vipp1

Plasmid

#223815

Purpose

Bacterial expression of Vipp1 with C-terminal 6x His-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Mar;32(3):555-

Insert

Vipp1

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET50delCT_Vipp1_H1-6

Plasmid

#223816

Purpose

Bacterial expression of Vipp1 H1-6 with C-terminal 6x His-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Mar;32(3):555-

Insert

Vipp1

Tags

6xHis

Expression

Bacterial

Mutation

truncation of helix 0

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-empty

Plasmid

#239099

Purpose

pGL3 luciferase vector with no promoter.

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Type

Empty backbone

Use

Luciferase

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4-C-EF1α

Plasmid

#239097

Purpose

Empty pcDNA4-C backbone with EF1α promoter.

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Type

Empty backbone

Expression

Mammalian

Promoter

EF1α

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET50del_SynPspA

Plasmid

#223818

Purpose

Bacterial expression of SynPspA with N-terminal 6xHis-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Jan;32(1):23-3

Insert

SynPspA

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pGTPA-lb

Plasmid

#171528

Purpose

Plasmid contains the Cas12k gRNA for mutant library construction

Depositor

Quanjiang Ji

Article

Chen et al Cell Rep. 2021 Aug 31;36(9):109635. doi:

Insert

Cas12k gRNA

Use

CRISPR

Promoter

Constitutive promoter J23119

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pGTPA

Plasmid

#171525

Purpose

Plasmid contains the Cas12k gRNA and transposon donor cassette

Depositor

Quanjiang Ji

Article

Chen et al Cell Rep. 2021 Aug 31;36(9):109635. doi:

Insert

Cas12k gRNA, transposon donor cassette

Use

CRISPR

Promoter

Constitutive promoter J23119

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA-RMP64 (pAVA3500)

Plasmid

#239237

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP64

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP64

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA-RMP24 (pAVA3547)

Plasmid

#239240

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP24

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP24

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA-RMP24 (pAVA3555)

Plasmid

#239242

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP24

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP24

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA-RMP24 (pAVA3563)

Plasmid

#239258

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP24

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP24

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA-RMP64 (pAVA3572)

Plasmid

#239259

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP64

Depositor

Andrei Alexandrov

Article

Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

Insert

U6-driven sgRNA targeting RMP64

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pZmSWEET13a:ZmSWEET13a:GUS

Plasmid

#159535

Purpose

expresses ß--glucuronidase in cells expressing ZmSWEET13a

Depositor

Wolf Frommer

Article

Bezrutczyk et al Plant Cell. 2021 May 5;33(3):531-547. do

Insert

ZmSWEET13a

Tags

GUS

Expression

Bacterial and Plant

Promoter

native SWEET13a promoter

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1369

Plasmid

#239828

Purpose

WT OrufIscB mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1376

Plasmid

#239829

Purpose

WT OrufIscB omegaRNA mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB omegaRNA scaffold

Expression

Mammalian

Promoter

human U6

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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GB3471

Plasmid

#215244

Purpose

CDS of HispS from N. nambi codon optimized for N.benthamiana. This gene is the first step of the bioluminiscente pathway.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

HispS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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