We narrowed to 27,857 results for: sta

1. pJ151-HDR

   Plasmid

   #113631

   Purpose

   Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.

   Depositor

   Alex Kentsis

   Inserts

   Left homology arm
   Puromycin resistance gene
   Venus fluorescence gene
   Thymidine kinase
   Right homology arm
   

   Use

   Cre/Lox; Homology recombination selection vector

   Expression

   Bacterial and Mammalian

   Promoter

   EF1a promoter, EF1a promoter (T2A cleaving sequen…

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNP1 sfGFP toehold switch

   Plasmid

   #107355

   Purpose

   Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 sfGFP trigger is co-expressed

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   toehold 7 + sfGFP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmiRFP720-tubulin

   Plasmid

   #136574

   Purpose

   Human alpha-tubulin labeling with a near-infrared fluorescent protein miRFP720

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   miRFP720-tubulin
   

   Tags

   miRFP720

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC118-FLIP-FlpE-Puro

   Plasmid

   #84539

   Purpose

   a mock vector for generating FLIP-FlpE targeting vectors

   Depositor

   Bon-Kyoung Koo

   Article

   Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

   Insert

   FLIP-FlpE - Puro
   

   Use

   Cre/Lox and Mouse Targeting

   Available Since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMM119

   Plasmid

   #127213

   Purpose

   BeYDV replicon with constitutive GFP expression, WUS2, sgRNA targeting PDS

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   GFP, WUS2, sgRNA
   

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBbS8c-ddcpf1-Δ

   Plasmid

   #153039

   Purpose

   For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   Ascpf1
   

   Expression

   Bacterial

   Mutation

   E993A

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBacPAK p110a WT

   Plasmid

   #55722

   Purpose

   His3 GST tagged WT p110 alpha

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   p110 alpha (Pik3ca Mouse)
   

   Tags

   His3 GST

   Promoter

   polyhedrin promoter

   Available Since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

8. FusionRed-Lifeact-7

   Plasmid

   #54778

   Purpose

   Localization: Actin, Excitation: 580, Emission: 608

   Depositor

   Michael Davidson

   Article

   Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

   Type

   Empty backbone

   Tags

   FusionRed-Lifeact

   Expression

   Mammalian

   Available Since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pLentiCRISPR V2 sgIREB2_2

   Plasmid

   #169886

   Purpose

   Disrupt IREB2

   Depositor

   Richard Possemato

   Article

   Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

   Insert

   sgRNA targeting IREB2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-NKX3.1

    Plasmid

    #42897

    Depositor

    Edward Gelmann

    Article

    Steadman et al Nucleic Acids Res. 2000 Jun 15;28(12):23

    Insert

    NKX3.1 (NKX3-1 Human)
    

    Expression

    Mammalian

    Available Since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

11. Tol2-lyzC-mito-Dendra2

    Plasmid

    #107590

    Purpose

    Expresses mitochondria localized Dendra2 in zebrafish neutrophils

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    mito-Dendra2
    

    Tags

    mitochondira localization sequence

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRF0G-IodoY

    Plasmid

    #113645

    Purpose

    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    IodoYRS and tRNA gene
    

    Use

    Synthetic Biology

    Available Since

    July 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pMM146

    Plasmid

    #127218

    Purpose

    BeYDV replicon with constitutive Luc expression, BBM, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, BBM, sgRNA
    

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV/hSyn-SCLM

    Plasmid

    #216760

    Purpose

    AAV2 transfer vector with the human synapsin I promoter for neuronal expression of SuperClomeleon

    Depositor

    George Augustine

    Insert

    SuperClomeleon
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S30R and 11 AA deletion in the Cerulean CFP moiet…

    Promoter

    hSyn (human synapsin I)

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAB078d5

    Plasmid

    #79204

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) luxAB; Ppro1 434cI
    

    Expression

    Bacterial

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. LTR5HS S. aureus scaffold CARGO array

    Plasmid

    #191317

    Purpose

    Contains an array of guide RNAs targeting LTR5Hs elements

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Insert

    Array of guide RNAs
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHCKan-phoAp-GFPuv

    Plasmid

    #139072

    Purpose

    Low phosphate inducible promoter phoAp for GFP expression

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

    Insert

    phoAp-GFPuv
    

    Expression

    Bacterial

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMM136

    Plasmid

    #127217

    Purpose

    BeYDV replicon with constitutive Luc expression, MP-delta, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, MP-delta, sgRNA
    

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Dendra2-Golgi-7

    Plasmid

    #57722

    Purpose

    Localization: Golgi, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Golgi (B4GALT1 Human)
    

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. PBMBP-eGFP

    Plasmid

    #40976

    Depositor

    Joseph Loturco

    Article

    Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

    Insert

    MBP promoter fragment (Mbp Mouse)
    

    Expression

    Mammalian

    Available Since

    May 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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