We narrowed to 31,198 results for: PLE;
-
Plasmid#193655PurposeExpression of base editor RR-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertRR-dLbCas12a-hA3A-Y130F, EGFP
ExpressionMammalianMutationdLbCas12a-G532R/K595R, hAPOBEC3A-Y130FPromoterCMVAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP
Plasmid#194890PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-1 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_K365A_R368A
U6-crRNA1
UseLentiviralTagsHAMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available SinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_Q418A-HA-P2A-tagBFP
Plasmid#194895PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-Q418A mutant and crRNA-2 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_Q418A
U6-crRNA2
UseLentiviralTagsHAExpressionMammalianMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ3A
Plasmid#197513PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-3 σ3A
TagsGAL4-DNA binding domain fragment, HA tag, and nuc…ExpressionYeastMutationsilent substitution in codon 2 of tyrosinase tail…PromoterADH1 and MET25Available SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP
Plasmid#194889PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-1 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_WT
U6-crRNA1
UseLentiviralTagsHAExpressionMammalianMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_Q418A-HA-P2A-tagBFP
Plasmid#194891PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-Q418A mutant and crRNA-1 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_Q418A
U6-crRNA1
UseLentiviralTagsHAExpressionMammalianMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_R396A-HA-P2A-tagBFP
Plasmid#194896PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-R396A mutant and crRNA-2 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_R396A
U6-crRNA2
UseLentiviralTagsHAExpressionMammalianMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_R396A-HA-P2A-tagBFP
Plasmid#194888PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-R396A mutant and crRNA-1 and -2 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_R396A
U6-crRNA1and2
UseLentiviralTagsHAExpressionMammalianMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_Q418A-HA-P2A-tagBFP
Plasmid#194887PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-Q418A mutant and crRNA-1 and -2 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_Q418A
U6-crRNA1and2
UseLentiviralTagsHAExpressionMammalianMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available SinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP
Plasmid#194893PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-2 targeting the human MECP2 locus.DepositorInsertsdLbCpf1-NLS-hCTCF_WT
U6-crRNA2
UseLentiviralTagsHAExpressionMammalianMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available SinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only