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Plasmid#104403Purposetransient expression of dCas9-DNMT3a fusion proteinDepositorInsertDNMT3a (DNMT3A Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pARNTL-donor
Plasmid#112384PurposeCRISPR donor plasmid to tag human transcription factor ARNTL with GFPDepositorInsertARNTL homology arms flanking EGFP-IRES-Neo cassette (BMAL1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTFAP2C-donor
Plasmid#113824PurposeCRISPR donor plasmid to tag human transcription factor TFAP2C with GFPDepositorInsertTFAP2C homology arms flanking EGFP-IRES-Neo cassette (TFAP2C Human)
UseCRISPRTagsExpressionMutationrs6025024, hg38:20:56638660:TTT/-PromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pISL1-donor
Plasmid#113820PurposeCRISPR donor plasmid to tag human transcription factor ISL1 with GFPDepositorInsertISL1 homology arms flanking EGFP-IRES-Neo cassette (ISL1 Human)
UseCRISPRTagsExpressionMutationUnknownPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDDIT3-donor
Plasmid#112300PurposeCRISPR donor plasmid to tag human transcription factor DDIT3 with GFPDepositorInsertDDIT3 homology arms flanking EGFP-IRES-Neo cassette (DDIT3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHOXB5-donor
Plasmid#113815PurposeCRISPR donor plasmid to tag human transcription factor HOXB5 with GFPDepositorInsertHOXB5 homology arms flanking EGFP-IRES-Neo cassette (HOXB5 Human)
UseCRISPRTagsExpressionMutationrs2288276, rs7406798, rs36049616, rs3087996PromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCLOCK-donor
Plasmid#112310PurposeCRISPR donor plasmid to tag human transcription factor CLOCK with GFPDepositorInsertCLOCK homology arms flanking EGFP-IRES-Neo cassette (CLOCK Human)
UseCRISPRTagsExpressionMutationhg38:4:55435551:A>G, hg38:4:55435749:G>APromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF416-donor
Plasmid#112372PurposeCRISPR donor plasmid to tag human transcription factor ZNF416 with GFPDepositorInsertZNF416 homology arms flanking EGFP-IRES-Neo cassette (ZNF416 Human)
UseCRISPRTagsExpressionMutationhg38:19:57572882:A>G,hg38:19:57572932:C>G,h…PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEHF-donor
Plasmid#113817PurposeCRISPR donor plasmid to tag human transcription factor EHF with GFPDepositorInsertEHF homology arms flanking EGFP-IRES-Neo cassette (EHF Human)
UseCRISPRTagsExpressionMutationrs3818514, rs11032804, hg38:11:34659087:G>APromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBCL6-donor
Plasmid#112389PurposeCRISPR donor plasmid to tag human transcription factor BCL6 with GFPDepositorInsertBCL6 homology arms flanking EGFP-IRES-Neo cassette (BCL6 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSRF-donor
Plasmid#112362PurposeCRISPR donor plasmid to tag human transcription factor SRF with GFPDepositorInsertSRF homology arms flanking EGFP-IRES-Neo cassette (SRF Human)
UseCRISPRTagsExpressionMutationhg38:6:43178470:C>A, hg38:6:43178514:G>APromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF766-donor
Plasmid#112352PurposeCRISPR donor plasmid to tag human transcription factor ZNF766 with GFPDepositorInsertZNF766 homology arms flanking EGFP-IRES-Neo cassette (ZNF766 Human)
UseCRISPRTagsExpressionMutationhg38:19:52290357:G>T, hg38:19:52290373:A>G,…PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVEZF1-donor
Plasmid#112341PurposeCRISPR donor plasmid to tag human transcription factor VEZF1 with GFPDepositorInsertVEZF1 homology arms flanking EGFP-IRES-Neo cassette (VEZF1 Human)
UseCRISPRTagsExpressionMutationrs999907002, hg38:17:57975308:A>T, hg38:17:579…PromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pELK3-donor
Plasmid#112322PurposeCRISPR donor plasmid to tag human transcription factor ELK3 with GFPDepositorInsertELK3 homology arms flanking EGFP-IRES-Neo cassette (ELK3 Human)
UseCRISPRTagsExpressionMutationhg38:12:96266960:A>T, rs760335647, rs6538704PromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDG330
Plasmid#100898PurposeSpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.DepositorInsertshumanized CRISPR associated protein 9 Nickase
U6-gRNA scaffold 1
U6-gRNA scaffold 2
UseCRISPR and Mouse TargetingTags3xFLAGExpressionMammalianMutationPromoterCBh and U6Available sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJB07
Plasmid#190481PurposeTargeting plasmid for 2-plasmid C. difficile mutagenesis systemDepositorInsertsUpstream & Downstream pyrE deletion region
gRNA
UseE. coli / b. subtilis - c. difficile shuttle vect…TagsExpressionMutationPromoterAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW-Tet-Cas9-BFP-PGK-Blast
Plasmid#196720PurposeTet-inducible expression of Cas9-T2A-TagBFPDepositorInsertCas9-T2A-TagBFP
UseCRISPR and LentiviralTags3xFLAG-SV40NLS and Nucleoplasmin NLSExpressionMutationPromoterTRE, PGKAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro
Plasmid#121174PurposeDonor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell linesDepositorInsertMAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-dCas9-VP64-MS2-VP64-ZsG
Plasmid#192665Purpose3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1DepositorInsertdCas9-VP64, MS2-VP64, ZsGreen1
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1aAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG-ABEmax-P2A-EGFP-ires-puro
Plasmid#121170PurposeCAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.DepositorInsertABEmax-P2A-EGFP-ires-puro
UseCRISPRTagsP2A-EGFP-ires-puroExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only