We narrowed to 22,749 results for: emb

1. pCAG-memRFP

   Plasmid

   #224564

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and membrane RFP reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   mRFP1
   

   Use

   Unspecified

   Tags

   Membrane localization signal

   Expression

   Mutation

   Codon optimized

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCVhygro-F-G9a

   Plasmid

   #41721

   Purpose

   Retroviral expression of human G9a in mammalian cells

   Depositor

   Kai Ge

   Article

   Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

   Insert

   G9a (EHMT2 Human)
   

   Use

   Retroviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUPD2

   Plasmid

   #68161

   Purpose

   Universal domesticator plasmid for level 0 parts

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   empty vector
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. 2C-3XtbGFP-PEST

   Plasmid

   #69072

   Purpose

   Expresses 3XturboGFP-NLS-PEST under MERVL_LTR

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

   Insert

   turboGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-hNINJ1-GFP

   Plasmid

   #208779

   Purpose

   Inducibly expresses human NINJ1 tagged with GFP

   Depositor

   Petr Broz

   Article

   Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

   Insert

   NINJ1 (NINJ1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV-mNINJ1-GFP

   Plasmid

   #208776

   Purpose

   MSCV vector expressing WT mouse NINJ1

   Depositor

   Petr Broz

   Article

   Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

   Insert

   NINJ1 (Ninj1 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pD-MSP1D1

   Plasmid

   #161607

   Purpose

   Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

   Insert

   D-MSP1D1
   

   Use

   Tags

   7-His tag

   Expression

   Bacterial

   Mutation

   mutant of MSP1D1; all tryptophans were replaced b…

   Promoter

   T7

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-MPAct-mRuby3-IRES-Puro

   Plasmid

   #155231

   Purpose

   lentiviral MPAct construct

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   Lentiviral expression of MPAct-mRuby sensor
   

   Use

   Lentiviral

   Tags

   mRuby3

   Expression

   Mutation

   Promoter

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUD-MSP1D1

   Plasmid

   #161608

   Purpose

   Bacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

   Insert

   UD-MSP1D1
   

   Use

   Tags

   7-His tag

   Expression

   Bacterial

   Mutation

   mutant of MSP1D1; all tryptophans and tyrosines w…

   Promoter

   T7

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLNCX2-mCherry-VPS4A

    Plasmid

    #115334

    Purpose

    expresses mCherry-VPS4A in mammalian cells

    Depositor

    Sanford Simon

    Article

    Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

    Insert

    VPS4A (VPS4A Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    silent mutation in position 4050 C to T

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNMT

    Plasmid

    #42578

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    N-myristoyl tranferase (NMT1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    two silent mutations T690C and T942C

    Promoter

    T7

    Available since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pLck-mVenus-C1

    Plasmid

    #84337

    Purpose

    Expresses a mVenus tagged with a membrane localization sequence

    Depositor

    Dorus Gadella

    Article

    van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

    Insert

    Lck (MGCVCSSNPELPVAT)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-Ran-T24N-mRFP1-polyA

    Plasmid

    #104561

    Purpose

    Express Ran T24N mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran (Ran Mouse)
    

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    T24N

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-Ran-Q69L-mRFP1-polyA

    Plasmid

    #104560

    Purpose

    Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran (Ran Mouse)
    

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    Q69L

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV-EGFP-ssHRP-TM

    Plasmid

    #31156

    Depositor

    Hollis Cline

    Article

    Li et al Front Neural Circuits. 2010 Feb 26;4:6.

    Insert

    ssHRP-TM
    

    Use

    Membrane targeted hrp (mhrp)

    Tags

    Expression

    Mammalian

    Mutation

    includes signal sequence and a transmembrane doma…

    Promoter

    CMV promoter including immediate early promoter/e…

    Available since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pLV-MPAct-mCit-IRES-Neo

    Plasmid

    #155230

    Purpose

    lentiviral MPAct construct

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    Lentiviral expression of MPAct-mCitrine sensor
    

    Use

    Lentiviral

    Tags

    mCitrine

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-RanWT-mRFP1-polyA

    Plasmid

    #59750

    Purpose

    Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran-mRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUB-MSP1D1

    Plasmid

    #161609

    Purpose

    Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

    Insert

    UD-MSP1D1
    

    Use

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    mutant of MSP1D1 ( Q138W, L181W, Y236W)

    Promoter

    T7

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-Nup37-EGFP-polyA

    Plasmid

    #104562

    Purpose

    Express Nup37-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Nup37 (Nup37 Mouse)
    

    Use

    Tags

    EGFP tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-EGFP-ELYS-polyA

    Plasmid

    #59746

    Purpose

    Express EGFP-ELYS in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    EGFP-ELYS
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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