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We narrowed to 4,839 results for: pcr

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Showing: 961 - 980 of 4839 results

  3. pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC

    Plasmid
    #140198
    Purpose
    Episomal expression of dLbCpf1(D156R)-VPR, variant for culture at room temperature. Filamentous fungi vector with AMA1 and riboB selection marker.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCas12a(D156R)-VPR
    Use
    CRISPR and Synthetic Biology; Expression in asper…
    Tags
    VPR (VP64-p65-Rta), NLS
    Mutation
    D832A DNase deactivated, D156R for improved activ…
    Promoter
    PgpdA
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140198-plasmid-map-sequence-id-309713

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  4. pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC

    Plasmid
    #140197
    Purpose
    Episomal expression of dLbCas12a-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCas12a-VPR
    Use
    CRISPR and Synthetic Biology; Expression in asper…
    Tags
    VPR (VP64-p65-Rta), NLS
    Mutation
    D832A DNase deactivated
    Promoter
    PgpdA
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140197-plasmid-map-sequence-id-309708

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  6. pCRI008-PgpdA-Cas12aScaffold-BsmbI-Cas12aScaffold-TrcpT

    Plasmid
    #140201
    Purpose
    Fungal vector for one-step cloning of LbCas12a crRNA arrays and expression. Pgpda and pyroA are BsmbI domesticated.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Cloning of crrna an…
    Tags
    dLbCas12a crRNA scaffold
    Promoter
    PgpdA
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140201-plasmid-map-sequence-id-310178

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  11. pOpen-THEAQpolA

    Plasmid
    #165512
    Purpose
    DNA polymerase used in routine PCR method because of its high thermostability, standard testing, screening and output testing.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Taq DNA Polymerase
    Use
    Synthetic Biology
    Available since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165512-plasmid-map-sequence-id-339400

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  12. pCMVlac-dirTopo-AU1-aGFP

    Plasmid
    #61971
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    61971-plasmid-map-sequence-id-101286

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  13. pCMVlac-dirTopo-P2A-aGFP

    Plasmid
    #61972
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    61972-plasmid-map-sequence-id-101285

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  14. pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI

    Plasmid
    #140202
    Purpose
    CRISPRa proof-of-concept test target with fluorescent reporter. PelcA is fused to mCherry, with low basal expression in Aspergillus nidulans. Fungal vector with AMA1 and pyrG selection marker.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Use
    CRISPR and Synthetic Biology; Proof-of-concept te…
    Mutation
    G174D
    Promoter
    PelcA
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140202-plasmid-map-sequence-id-310118

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  15. pCRI006-pYFAC-riboB-PgpdA-dSpCas9-VPR-TtrpC

    Plasmid
    #140199
    Purpose
    Episomal expression of dSpCas9-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Synthetic Biology; Expression in asper…
    Tags
    VPR (VP64-p65-Rta), NLS
    Promoter
    PgpdA
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140199-plasmid-map-sequence-id-309723

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  16. pJMP15011_pCRISPRtOE-noP-hyg

    Plasmid
    #225859
    Purpose
    Tn6677 transposon with guide RNA (BsaI site) and hyg resistance expression; no promoter.
    Depositor
    Jason Peters
    Publication
    Banta et al bioRxiv 2024.03.01.582922
    Insert
    CRISPRtOE transposon with gRNA expression cassette (no guide) and no outward facing promoter
    Use
    Synthetic Biology; Crispr associated transpositio…
    Expression
    Bacterial
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    225859-plasmid-map-sequence-id-448425

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  17. pJMP15021_pCRISPRtOE-PH-hyg-LZ1

    Plasmid
    #225861
    Purpose
    Tn6677 transposon with guide RNA (LZ1 guide) and hyg resistance expression; promoter H.
    Depositor
    Jason Peters
    Publication
    Banta et al bioRxiv 2024.03.01.582922
    Insert
    CRISPRtOE transposon with gRNA expression cassette (LZ1 guide) and synthetic promoter H
    Use
    Synthetic Biology; Crispr associated transpositio…
    Expression
    Bacterial
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    225861-plasmid-map-sequence-id-448427

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  18. pJMP10751_pCRISPRt-T-hyg-T03

    Plasmid
    #225844
    Purpose
    Tn6677 transposon with guide RNA (mscar T03 spacer) and hyg resistance expression.
    Depositor
    Jason Peters
    Publication
    Banta et al bioRxiv 2024.03.01.582922
    Insert
    CRISPRt transposon with gRNA expression cassette (mScar-T03 guide)
    Use
    Synthetic Biology; Crispr associated transpositio…
    Expression
    Bacterial
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    225844-plasmid-map-sequence-id-448363

    Enlarge

  19. pJMP10471_pCRISPRtOE-noP-gen

    Plasmid
    #225845
    Purpose
    Tn6677 transposon with guide RNA (BsaI site) and gm resistance expression; no promoter.
    Depositor
    Jason Peters
    Publication
    Banta et al bioRxiv 2024.03.01.582922
    Insert
    CRISPRtOE transposon with gRNA expression cassette (no guide) and no outward facing promoter
    Use
    Synthetic Biology; Crispr associated transpositio…
    Expression
    Bacterial
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    225845-plasmid-map-sequence-id-448365

    Enlarge

  20. pJMP10541_pCRISPRtOE-PH-gen-LZ1

    Plasmid
    #225847
    Purpose
    Tn6677 transposon with guide RNA (LZ1 guide) and gm resistance expression; promoter H.
    Depositor
    Jason Peters
    Publication
    Banta et al bioRxiv 2024.03.01.582922
    Insert
    CRISPRtOE transposon with gRNA expression cassette (LZ1 guide) and synthetic promoter H
    Use
    Synthetic Biology; Crispr associated transpositio…
    Expression
    Bacterial
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    225847-plasmid-map-sequence-id-459303

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Showing: 961 - 980 of 4839 results