We narrowed to 11,581 results for: Vars;

1. pYPQ121-mTCABE-T

   Plasmid

   #218173

   Purpose

   Express plastid monomeric TALE-linked CABE-T, composed of TALE, a catalytically impaired, full length DddA variant (E1347A), a TadA-derived deaminase, and UGI

   Depositor

   Yiping Qi

   Article

   Wang et al Plant Biotechnol J. 2024 May 6. doi: 10.

   Insert

   TALE-Intron-TadAC-DddA full (E1347A)-UGI
   

   Use

   Plastid base editing

   Expression

   Plant

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. P1K-3HA-Atg8∆GR-MET15

   Plasmid

   #207033

   Purpose

   Expression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Tags

   3xHA

   Expression

   Yeast

   Mutation

   ∆GR

   Promoter

   pATG8

   Available Since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Dox-SOScat

   Plasmid

   #207329

   Purpose

   PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized catalytic domain of SOS (SOScat)

   Depositor

   Jared Toettcher

   Article

   Underhill et al Development. 2023 Aug 15;150(16):dev2016

   Insert

   son of sevenless homolog 2 variant, catalytic domain
   

   Tags

   myristoylation tag - BFP

   Expression

   Bacterial

   Mutation

   range: 593 to 1078

   Promoter

   Tet Response Element

   Available Since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDL28_Cas9-His Δcys E532C/E1207C

   Plasmid

   #206289

   Purpose

   Bacterial expression of SpCas9 Δcys E532C and E1027C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, E532C, C574S, E1207C

   Promoter

   T7

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDL25_Cas9-His Δcys E1207C

   Plasmid

   #206286

   Purpose

   Bacterial expression of Cas9-His Δcys E1027C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, C574S, E1207C

   Promoter

   T7

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDL23_Cas9-His Δcys E945C

   Plasmid

   #206284

   Purpose

   Bacterial expression of Cas9-His Δcys E945C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, C574S, E945C

   Promoter

   T7

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDL24_Cas9-His Δcys E1026C

   Plasmid

   #206285

   Purpose

   Bacterial expression of Cas9-His Δcys E1026C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, C574S, E1026C

   Promoter

   T7

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDL27_Cas9-His Δcys E532C/E945C

   Plasmid

   #206288

   Purpose

   Bacterial expression of SpCas9 Δcys E532C and E945C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, E532C, C574S, E945C

   Promoter

   T7

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDL26_Cas9-His Δcys M1C/E532C

   Plasmid

   #206287

   Purpose

   Bacterial expression of SpCas9 Δcys M1C and E532C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   M1C, C80S, E532C, C574S

   Promoter

   T7

   Available Since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDL22_Cas9-His Δcys E532C

    Plasmid

    #206283

    Purpose

    Bacterial expression of Cas9-His Δcys E532C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, E532C, C574S

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG1.1-HA/Ppp1cc2

    Plasmid

    #199626

    Purpose

    Expression vector of mouse PPP1CC2. HA tag at N-terminus.

    Depositor

    Masahito Ikawa

    Article

    Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

    Insert

    Ppp1cc mRNA (variant X1) (Ppp1cc Mouse)
    

    Tags

    HA-tag

    Promoter

    CAG

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc5.1A-DmXRN1-dsRNAres-V5His6_N

    Plasmid

    #147027

    Purpose

    Insect Expression of DmXRN1-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

    Insert

    DmXRN1-dsRNAres (pcm Fly)
    

    Expression

    Insect

    Mutation

    one silent mtuation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAFMW-Nbr-CM

    Plasmid

    #188585

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler Catalytic mutant
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    deleted first ATG; D435A [GAT=>GCC]; E435A [GA…

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAFMW-Nbr[N1-383]

    Plasmid

    #188586

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler N-terminal domain
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    deleted first ATG

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAFMW-Nbr[C399-625]

    Plasmid

    #188587

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler C-terminal domain
    

    Tags

    FLAG-Myc

    Expression

    Insect

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAc5.1B-lambdaN-HA-DmXRN1_A

    Plasmid

    #145879

    Purpose

    Insect Expression of DmXRN1

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmXRN1 (pcm Fly)
    

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. CD44U-c011

    Plasmid

    #175454

    Purpose

    Protein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649. Can be biotinylated.

    Depositor

    Opher Gileadi

    Insert

    CD44 (CD44 Human)
    

    Tags

    AviTag-His6

    Expression

    Mammalian

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJH580

    Plasmid

    #172164

    Purpose

    pylBCD variant in pBAD backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Para (pylBCD 3f2v2+B); AraC
    

    Expression

    Bacterial

    Mutation

    PylB(N61S, E84K, E122K, E178K, G188R, E251D, G332…

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJH61

    Plasmid

    #172143

    Purpose

    Accessory Plasmid for PACE of pylBCD variants

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Ppsp gIII.1TAG, luxAB; Plpp [eG SD8] pylS; PproK pylT
    

    Expression

    Bacterial

    Mutation

    gIII(P29*); [strong promoter eG, strong RBS SD8] …

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSUMO chicken-cPLA2-C2-domain

    Plasmid

    #170753

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

    Insert

    PLA2G4A-C2 domain (PLA2G4A Chicken)
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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