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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,055 results for: crispr grnas

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Showing: 9801 - 9820 of 14055 results

  3. pAC-crRNA-Km

    Plasmid
    #158712
    Purpose
    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    AcGFP (for crRNA cloning)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    TetO
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    158712-plasmid-map-sequence-id-321925

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  5. pFH6

    Plasmid
    #86555
    Purpose
    Subcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiency
    Depositor
    Andreas Weber
    Article
    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:
    Insert
    U6-26p::sgRNA scaffold
    Use
    CRISPR; Subcloning
    Promoter
    Arabidopsis U6-26 promoter
    Available Since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86555-plasmid-map-sequence-id-176427

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  7. pPBO.ACT005

    Plasmid
    #182715
    Purpose
    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region
    Depositor
    Joseph Schoeniger
    Article
    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666
    Insert
    CasRx crRNA cloning backbone
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Catalytically deactivated R295A, H300A, R849A, H8…
    Promoter
    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)
    Available Since
    Aug. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    182715-plasmid-map-sequence-id-361296

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  13. pGMF1-M

    Plasmid
    #242203
    Purpose
    OsU6-2p::sgRNA scaffold in L1 vector backbone, for subcloning of 1st 20 bp target sequence. For use in monocots.
    Depositor
    Kate Creasey Krainer
    Article
    Abate et al bioRxiv 2025.11.09.687425
    Insert
    Level1 OsU6-2p::sgRNA1 for subcloning of 20 bp target sequence
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Promoter
    OsU6-2p
    Available Since
    Nov. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242203-plasmid-map-sequence-id-490170

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  14. pGMF2-M

    Plasmid
    #242204
    Purpose
    OsU6-2p::sgRNA scaffold in L1 vector backbone, for subcloning of 2nd 20 bp target sequence. For use in monocots.
    Depositor
    Kate Creasey Krainer
    Article
    Abate et al bioRxiv 2025.11.09.687425
    Insert
    Level1 OsU6-2p::sgRNA2 for subcloning of 20 bp target sequence
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Promoter
    OsU6-2p
    Available Since
    Nov. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242204-plasmid-map-sequence-id-490169

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  15. pGMF1-D

    Plasmid
    #242205
    Purpose
    AtU6-26p::sgRNA scaffold in L1 vector backbone, for subcloning of 1st 20 bp target sequence. For use in dicots.
    Depositor
    Kate Creasey Krainer
    Article
    Abate et al bioRxiv 2025.11.09.687425
    Insert
    Level1 AtU6-26p::sgRNA1 for subcloning of 20 bp target sequence
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Promoter
    AtU6-26p
    Available Since
    Nov. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242205-plasmid-map-sequence-id-490168

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  18. pPBO.ACT004

    Plasmid
    #182714
    Purpose
    Constituitvely expressed CasRx crRNA cloning vector, extended 3' terminator region
    Depositor
    Joseph Schoeniger
    Article
    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666
    Insert
    CasRx crRNA cloning backbone
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Catalytically deactivated R295A, H300A, R849A, H8…
    Promoter
    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)
    Available Since
    Aug. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    182714-plasmid-map-sequence-id-364194

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Showing: 9801 - 9820 of 14055 results