We narrowed to 31,198 results for: PLE;
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Plasmid#213299PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only
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HA-MBP-TEVpCS-ADGRG6 CTF-FLAG
Plasmid#217704PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGR6 (ADGRG6 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-840Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRM166 LKB (Luciferase mKate2 Blast)
Plasmid#183502PurposeExpresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.DepositorInsertsFirefly Luciferase
mKate2
BSD
UseLentiviral and LuciferaseTagsP2A linker and SV40 NLS with GGS linkerExpressionMammalianPromoterMND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMIR-FLAG-MLL-AF9
Plasmid#71444PurposeRetroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2DepositorAvailable SinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Opto-A2AR
Plasmid#106066PurposeExpression of a chimeric putative G-protein coupled receptor (Rhodopsin-A2AR; Opto-A2AR; F8; 719)DepositorInsertOpto-A2AR (ADORA2A Bovine, Human)
TagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTET GFP10-FRB/FKBP-GFP11
Plasmid#182497PurposeTripartite split-GFP. E. coli vector. Inducible coexpression of GFP10-FRB and FKBP-GFP11 coiled coilsDepositorInsertsTagssplit GFP10 tripartite and split GFP11 tripartiteExpressionBacterialPromoterTETAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVNP2.0-SpCas9
Plasmid#206880PurposeExpresses FLAG-tagged SpCas9 fused to the N-terminus of Gag/GagPol harbouring an intervening phospholipase C-δ1 pleckstrin homology (PH) domainDepositorAvailable SinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-eGFP-IRESMuro
Plasmid#82504PurposeVector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInserteGFP-IRESMuro
UseSynthetic BiologyExpressionMammalianAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 c-SRC (WT)
Plasmid#42202DepositorInsertc-SRC (SRC Human)
ExpressionMammalianMutationL176F mutation compared to reference sequence NM_…PromoterCMVAvailable SinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
GPRC5C-DuET
Plasmid#213297PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only