We narrowed to 15,995 results for: LEA

1. pV1010

   Plasmid

   #111423

   Purpose

   gRNA plasmid for cleavage of Candida albicans ADE2 - integrates at RPS1 (Part of Duet system)

   Depositor

   Gerald Fink

   Article

   Vyas et al Sci Adv. 2015;1(3):e1500248.

   Insert

   sgADE-NT2
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBW-m4133279

   Plasmid

   #152843

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;TapTag

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available Since

   July 30, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pGBW-m4133784

   Plasmid

   #152558

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHis-003

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available Since

   July 30, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. p3E-2a-6MTnlstdTomato

   Plasmid

   #67699

   Purpose

   3' entry clone with 6xmyc nuclear localized tdTomato

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3785-37

   Insert

   tdTomato
   

   Tags

   2A-6xMyc-NLS

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. mTagRFP-PCNA-19-SV40NLS-4

   Plasmid

   #57994

   Purpose

   Localization: Nucleus, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Tags

   mTagRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 4, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pEGFP-HA3-STONIN1

   Plasmid

   #177580

   Purpose

   Expresses Stonin1 in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Martina et al J Cell Biol. 2001 May 28;153(5):1111-20.

   Insert

   STONIN1
   

   Tags

   3XHA and GFP

   Expression

   Mammalian

   Mutation

   V334A- please see depositor comments

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCoofy61

   Plasmid

   #122013

   Purpose

   E.coli vector for parallel SLIC cloning containing N-terminal His6-eGFP with PreScission Cleavage site

   Depositor

   Peggy Stolt-Bergner, Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Tags

   His6-eGFP

   Expression

   Bacterial

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMtB

   Plasmid

   #32096

   Depositor

   Christopher Buck

   Article

   Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

   Insert

   MCV Small t antigen
   

   Expression

   Mammalian

   Mutation

   aberrant splicing leads to additional protein pro…

   Available Since

   Oct. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBW-m4046451

   Plasmid

   #145567

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp6

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pGBW-m4134552

    Plasmid

    #152652

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. TRIM33 (TRIM33B-c100)

    Plasmid

    #98243

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TRIM33B (TRIM33 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00628:PHD 889-932, pfam00439:Bromodomain 963-…

    Promoter

    T7

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGBW-m4133579

    Plasmid

    #152181

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    June 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pCHAP4

    Plasmid

    #206850

    Purpose

    A plasmid containing a 16,121 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector.

    Depositor

    Bogumil Karas

    Article

    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET22-6His-SUMO-Nsp10

    Plasmid

    #154960

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    

    Tags

    6His-Thrombin-SUMO

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    July 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pcDNA_DiCre2_59.F2

    Plasmid

    #60209

    Purpose

    Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(19-59)

    Depositor

    Jean-Paul Herman

    Insert

    Cre recombinase (CD207 Bacteriophage P1, Human, Synthetic)
    

    Tags

    FKBP12(F36V) and Nuclear localisation signal

    Expression

    Mammalian

    Mutation

    FKBP12 has the F36V mutation; amino acids 19-59 o…

    Promoter

    CMV

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGBW-m4133721

    Plasmid

    #152946

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pGBW-m4134371

    Plasmid

    #152394

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pGBW-m4134525

    Plasmid

    #152660

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHis-003

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    July 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGBW-m4133718

    Plasmid

    #152004

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 ORF8 protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF8 protein (ORF8 Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pTZ U6+27

    Plasmid

    #25713

    Purpose

    for nuclear expression of small RNAs in mammalian cells

    Depositor

    David Engelke

    Article

    Good et al Gene Ther. 1997 Jan . 4(1):45-54.

    Insert

    U6 snRNP (RNU6-1 Human)
    

    Expression

    Mammalian

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only