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pINTO-N-SA

Plasmid

#61723

Purpose

Inducible expression of proteins fused to an N-terminal monomeric streptavidin tag (mSA)

Depositor

Roberto Bonasio

Publication

Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

Type

Empty backbone

Tags

mSA

Expression

Mammalian

Promoter

T-REx (CMV + 2xTetO2)

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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W1

Plasmid

#21208

Depositor

Paul Khavari

Publication

Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

Insert

Mek1 (MAP2K1 Human)

Use

Subclone vector

Available since

July 24, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pFA6a-link-ymNeongreen-URA3

Plasmid

#168059

Purpose

Replaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FP

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeonGreen

Tags

ymNeonGreen

Expression

Yeast

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLF3FShC

Plasmid

#153083

Purpose

RMCE integration vector with self excising cassette

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMaCTag-P23

Plasmid

#120034

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Halo

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Halo

Use

Pcr template

Tags

Halo

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCN-PDC1-GFP

Plasmid

#45326

Depositor

Brendan Cormack

Publication

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Insert

yeGFP

Use

C. glabrata expression

Promoter

PDC1

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBRIT TAP

Plasmid

#17520

Depositor

Michael Rudnicki

Publication

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

TAP

Expression

Mammalian

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
GST-Keima-cb5

Plasmid

#137755

Purpose

Targets Keima to the endoplasmic reticulum. Used to monitor ER-phagy

Depositor

Carol Mercer

Publication

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

Keima-cb5

Tags

GST

Expression

Mammalian

Promoter

CMV

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCiPS

Plasmid

#60011

Purpose

Foamy virus helper plasmid expressing Pol, driven by a CMV promoter. Ampicillin resistant.

Depositor

David Russell

Publication

Trobridge et al Mol Ther. 2002 Sep;6(3):321-8.

Insert

Pol

Expression

Mammalian

Promoter

CMV

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pMRBAD-link-CGFP

Plasmid

#52733

Purpose

L-(+)-arabinose-inducible expression of link-CGFP negative control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Publication

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

link-CGFP

Expression

Bacterial

Promoter

PBAD

Available since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMYO

Plasmid

#34626

Depositor

Jesse Rinehart, Dieter Söll

Publication

Park et al Science. 2011 Aug 26;333(6046):1151-4.

Insert

Myoglobin

Tags

6xHIS

Expression

Bacterial

Mutation

codon-optimized

Promoter

lpp

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAc5.1C-FLuc-V5His6

Plasmid

#21183

Depositor

Elisa Izaurralde

Publication

Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

Insert

Firefly Luciferase

Use

Drosophila expression

Available since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pET3a-Ub-D77

Plasmid

#18896

Depositor

Cecile Pickart

Publication

Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

Insert

Ubiquitin-D77 (UBC Human)

Expression

Bacterial

Mutation

Aspartate residue is added to the C-terminus of t…

Available since

Sept. 10, 2008

Availability

Academic Institutions and Nonprofits only
AbVec2.1-mIglc2

Plasmid

#127156

Purpose

Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), lambda 2 constant region

Depositor

Hedda Wardemann

Publication

Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

Insert

Iglc2 (Iglc2 Mouse)

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLentiCRISPRv2 sgIRF3#5

Plasmid

#127642

Purpose

Knock-out of human IRF3

Depositor

Nicolas Manel

Publication

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

IRF3 sgRNA (IRF3 Human)

Use

Lentiviral

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HshnRNPK

Plasmid

#64923

Purpose

Expresses V5- and SBP-tagged hnRNPK in mammalian cells

Depositor

Elisa Izaurralde

Insert

hnRNPK (HNRNPK Human)

Tags

V5 and SBP

Expression

Mammalian

Promoter

CMV

Available since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1 Flag-Eya2

Plasmid

#49264

Purpose

expresses Flag tagged Eya2

Depositor

Heide Ford

Insert

Eya2 (EYA2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
q4wt

Plasmid

#24502

Depositor

Bruce Conklin

Publication

Conklin et al Nature. 1993 May 20. 363(6426):274-6.

Insert

Gq alpha

Tags

HA (see comments)

Expression

Mammalian

Available since

May 13, 2010

Availability

Academic Institutions and Nonprofits only
pCdrA-gfp(ASV)C

Plasmid

#111615

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

Depositor

Tim Tolker-Nielsen

Publication

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(ASV)-T0-T1

Expression

Bacterial

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHR-FKBP:mCherry-Rab7a

Plasmid

#72903

Purpose

Late endosome-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Publication

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Rab7a (RAB7A Human)

Use

Lentiviral

Tags

FKBP, mCherry

Expression

Mammalian

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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