We narrowed to 25,036 results for: Nes

1. pKSB-609 U6-sgRNA1 ATCC GGAA

   Plasmid

   #173671

   Purpose

   To clone and express a gRNA in Anopheles

   Depositor

   Eric Marois

   Article

   Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. mscv2.2-6mycFlaA

   Plasmid

   #60203

   Purpose

   6xMYC-tagged full-length FlaA from Legionella pneumophila, expressed under the constitutive, moderate-level retroviral LTR promoter for mammalian cell expression; IRES-GFP reporter downstream of FlaA

   Depositor

   Russell Vance

   Article

   Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

   Insert

   FlaA
   

   Use

   Retroviral

   Tags

   6xMYC

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. MIG(F)-RelA

   Plasmid

   #26986

   Depositor

   Stephen Smale

   Article

   Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

   Insert

   RelA (Rela Mouse)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET22b-T5-mad32TAG

   Plasmid

   #188997

   Purpose

   Express madanin-1 with TAG at 32 position

   Depositor

   Han Xiao

   Article

   Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

   Insert

   madanin-1 with TAG at 32 position
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABE Hygro ST6GalNaC5

   Plasmid

   #26981

   Depositor

   Joan Massague

   Article

   Bos et al Nature. 2009 Jun 18. 459(7249):1005-9.

   Insert

   ST6GalNaC5 (ST6GALNAC5 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

6. p1.1

   Plasmid

   #162737

   Purpose

   Protein expression in CHO cells

   Depositor

   Ivan Vorobiev

   Article

   Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCBLsgRNA1

   Plasmid

   #199727

   Purpose

   Golden Gate entry vector; 1st gRNA under OsU3 promoter

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   OsU3-sgRNA1 scaffold
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCBLsgRNA2

   Plasmid

   #199728

   Purpose

   Golden Gate entry vector; 2nd gRNA under OsU3 promoter

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   OsU3-sgRNA2 scaffold
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICH51266

   Plasmid

   #50267

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter (1.3 kb ), 35s (Cauliflower Mosaic Virus) + 5'UTR omega (Tobacco Mosaic Virus)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGGF012

    Plasmid

    #48849

    Purpose

    Provides a sulfadiazine resistance cassette as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    pMAS::SulfadiazineR::t35S
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    internal BsaI recognition sites in SulfR removed …

    Promoter

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGWB601NL3F10H

    Plasmid

    #141287

    Purpose

    No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, BASTA R for plant)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Type

    Empty backbone

    Use

    Luciferase; Gateway

    Tags

    FLAG (x3), HIS (x10), and NLUC

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMC-J6-dCas9-24XGP41-J9

    Plasmid

    #202006

    Purpose

    MoClo level 0 dCas9-24XGP41 coding sequence module

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Insert

    dCas9-24XGP41
    

    Use

    Synthetic Biology; Moclo cloning vector

    Tags

    GS linkers

    Expression

    Mutation

    Promoter

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. p415GPD-3xHA-GW

    Plasmid

    #63781

    Purpose

    yeast expression vector p415GPD modified such that it is Gateway converted and introduces a 3xHA tag at the N-terminus of the insert

    Depositor

    Susan Baserga

    Article

    McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

    Type

    Empty backbone

    Use

    Tags

    3xHA

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available Since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2+ Integrin beta 1b-mKate

    Plasmid

    #105975

    Purpose

    in vitro transcription, Full length zebrafish Integrin beta 1b tagged C terminally with mKate2 using Gateway cloning.

    Depositor

    Caren Norden

    Article

    Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

    Insert

    Integrinbeta1b
    

    Use

    Tags

    mKate

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. CBX4

    Plasmid

    #25237

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CBX4_02 (CBX4 Human)
    

    Use

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pZ147-BvCas12b-sgRNA-scaffold

    Plasmid

    #122447

    Purpose

    Expresses sgRNA for BvCas12b in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    U6-BvCas12b-sgRNA-scaffold, CMV-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. HDAC6 (3GV4)

    Plasmid

    #25297

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HDAC6_42 (HDAC6 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acid residues 1109-1215

    Promoter

    Available Since

    Aug. 16, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pMB66

    Plasmid

    #47946

    Purpose

    Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

    Expression

    Worm

    Mutation

    Promoter

    hsp-16.48

    Available Since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGGE001

    Plasmid

    #48839

    Purpose

    Provides the rbcS terminator as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    rbcS terminator
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMB-TPr

    Plasmid

    #61190

    Purpose

    Fluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    AtTIP1-1
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available Since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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