We narrowed to 14,571 results for: VARS

1. pCBh-T7-SpG-P2A-mTagBFP2(RAS3583)

   Plasmid

   #223110

   Purpose

   pCBh Human expression plasmid for SpG with with C-term BPNLS-P2A-mTagBFP2

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpG-P2A-mTagBFP2
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-mTagBFP2

   Expression

   Mammalian

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCBh-T7-SpCas9-P2A-mTagBFP2(RAS3575)

   Plasmid

   #223111

   Purpose

   pCBh Human expression plasmid for SpCas9 with with C-term BPNLS-P2A-mTagBFP2

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9-P2A-mTagBFP2
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-mTagBFP2

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-SpCas9-P2A-EGFP (MSP2582)

   Plasmid

   #223067

   Purpose

   pCAG human codon optimized wild-type SpCas9 expression plasmid with C-terminal NLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized SpCas9-P2A-EGFP
   

   Use

   CRISPR

   Tags

   NLS(SV40)-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiCRISPR V2 Neo sgITGB1_3

   Plasmid

   #233250

   Purpose

   Knock out of murine ITGB1

   Depositor

   David Bryant

   Article

   Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

   Insert

   ITGB1 (Itgb1 Mouse)
   

   Use

   Lentiviral

   Available Since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCRISPR V2 Neo sgITGB1_2

   Plasmid

   #233249

   Purpose

   Knock out of murine ITGB1

   Depositor

   David Bryant

   Article

   Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

   Insert

   ITGB1 (Itgb1 Mouse)
   

   Use

   Lentiviral

   Available Since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. EFS-SpdCas9-Dnmt3A/3L-V1

   Plasmid

   #222498

   Purpose

   Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) followed by P2A-mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Insert

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A

   Promoter

   EFS

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. EFS-SpdCas9-Dnmt3A/3L-V2

   Plasmid

   #222499

   Purpose

   Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) followed by P2A-mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Insert

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A

   Promoter

   EFS

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. EFS-SpdCas9-Dnmt3A/3L-V3

   Plasmid

   #222500

   Purpose

   Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) followed by P2A-mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Insert

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; R887E and E814…

   Promoter

   EFS

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TRE3GV-SpdCas9-Dnmt3A/3L-V1

   Plasmid

   #222503

   Purpose

   Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V1 (R887E) and hPGK promoter driven mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Inserts

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V1 (R887E) engineered fusion
   mCherry
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; R887E in Dnmt3A

   Promoter

   TRE3GV and hPGK

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TRE3GV-SpdCas9-Dnmt3A/3L-V2

    Plasmid

    #222505

    Purpose

    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) and hPGK promoter driven mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Inserts

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
    mCherry
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A

    Promoter

    TRE3GV and hPGK

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TRE3GV-SpdCas9-Dnmt3A/3L-V3

    Plasmid

    #222506

    Purpose

    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) and hPGK promoter driven mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Inserts

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
    mCherry
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; R887E and E814…

    Promoter

    TRE3GV and hPGK

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TRE3GV-SpdCas9-Dnmt3A/3L-catΔ

    Plasmid

    #222508

    Purpose

    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) and hPGK promoter driven mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Inserts

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
    mCherry
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; C706A and R832…

    Promoter

    TRE3GV and hPGK

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. IL2-AHDC2-Cherry in pmCherryN1

    Plasmid

    #187291

    Purpose

    Express pmCherry-IL2-AH-deltaC2

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Scraps interleukin-2 receptor alpha-subunit, anillin (IL2, ANLN Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    AH-deltaC2

    Promoter

    CMV

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONOR-MARINA

    Plasmid

    #223328

    Purpose

    DONOR vector for genomic integration of MARINA voltage sensitive indicator gene from Platisa et al., 2017 (10.1021/acschemneuro.6b00234).

    Depositor

    Jonathan Arias

    Article

    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

    Inserts

    MARINA
    KIR2.1 channel Golgi-to-plasma membrane trafficking signal
    

    Use

    CRISPR and Synthetic Biology

    Tags

    mCherry and super-ecliptic pHluorin

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSF_his6_sumo_hRNMT_RAM

    Plasmid

    #213528

    Purpose

    Expresses the human RNMT-RAM complex

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Inserts

    human RNMT (RNMT Human)
    RAM
    

    Tags

    His6-sumo

    Expression

    Bacterial

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSF_his6_sumo_SARS_CoV2_NSP14

    Plasmid

    #213518

    Purpose

    Expresses NSP14 of SARS-CoV-2

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Tags

    N-terminal His6-sumo

    Expression

    Bacterial

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSF_his6_sumo_SARS_CoV2_NSP14_V290A

    Plasmid

    #213522

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation V290A

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed V290 to A

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRSF_his6_sumo_SARS_CoV2_NSP14_N306K

    Plasmid

    #213523

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation N306K

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV2-NSP14 (ORF1ab SARS-CoV-2)
    

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed N306 to K

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSF_his6_sumo_SARS_CoV2_NSP14_P335S

    Plasmid

    #213524

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation P335S

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed P335 to S

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSF_his6_sumo_SARS_CoV2_NSP14_P335H

    Plasmid

    #213525

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation P335H

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed P335 to H

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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