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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,205 results for: LEA

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Showing: 9961 - 9980 of 12205 results

  1. pdHJ2

    Plasmid
    #248893
    Purpose
    Generation of double Holliday Junction DNA
    Depositor
    Stephen West
    Publication
    Ho et al Proc Natl Acad Sci U S A. 2022 May 3;119
    Insert
    synthetic DNA comprised of homologous sequences (to phagemid backbone), DNAzyme self-cleavage cassettes and precursor DNA
    Use
    Cloning and sequencing. as a phagemid, it allowsā€¦
    Available since
    Feb. 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    248893-plasmid-map-sequence-id-500691

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  3. pEvolvR-mCherry-Sniper-nCas9(D10A)-PolI5MĪ”

    Plasmid
    #249071
    Purpose
    Expresses nucleus-localized Sniper-nCas9 (D10A) fused to PolI5MĪ” and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    Sniper-nCas9-PolI5MĪ”
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease dā€¦
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249071-plasmid-map-sequence-id-496323

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  4. pEvolvR-mCherry-SuperFi-nCas9(D10A)-PolI5MĪ”

    Plasmid
    #249070
    Purpose
    Expresses nucleus-localized SuperFi-nCas9 (D10A) fused to PolI5MĪ” and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    SuperFi-nCas9-PolI5M?
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease dā€¦
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249070-plasmid-map-sequence-id-496322

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  5. pEvolvR-mCherry-HSC1.2-nCas9(D10A)-PolI5MĪ”

    Plasmid
    #249073
    Purpose
    Expresses nucleus-localized HSC1.2-nCas9 (D10A) fused to PolI5MĪ” and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    HSC1.2-nCas9-PolI5MĪ”
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease dā€¦
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249073-plasmid-map-sequence-id-496319

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  6. pEvolvR-mCherry-LZ3-nCas9(D10A)-PolI5MĪ”

    Plasmid
    #249072
    Purpose
    Expresses nucleus-localized LZ3-nCas9 (D10A) fused to PolI5MĪ” and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    LZ3-nCas9-PolI5MĪ”
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease dā€¦
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249072-plasmid-map-sequence-id-496318

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  7. pEvolvR-mCherry-HF1-nCas9(D10A)-PolI5MĪ”

    Plasmid
    #249075
    Purpose
    Expresses nucleus-localized HF1-nCas9 (D10A) fused to PolI5MĪ” and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    HF1-nCas9-PolI5MĪ”
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease dā€¦
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249075-plasmid-map-sequence-id-496315

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  8. pEvolvR-mCherry-Hypa-nCas9(D10A)-PolI5MĪ”

    Plasmid
    #249076
    Purpose
    Expresses nucleus-localized Hypa-nCas9 (D10A) fused to PolI5MĪ” and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    Hypa-nCas9-PolI5MĪ”
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease dā€¦
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249076-plasmid-map-sequence-id-496314

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  9. pEvolvR-mCherry-SpRY-nCas9(D10A)-PolI5MĪ”

    Plasmid
    #249074
    Purpose
    Expresses nucleus-localized SpRY-nCas9 (D10A) fused to PolI5MĪ” and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    SpRY-nCas9-PolI5MĪ”
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease dā€¦
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249074-plasmid-map-sequence-id-496313

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  10. pGGE-AtCLFset

    Plasmid
    #246807
    Purpose
    A GreenGate entry vector containing coding region of SET domain of AtCLF
    Depositor
    Danhua Jiang
    Insert
    AtCLFset (CLF Mustard Weed)
    Use
    Greengate cloning entry vector
    Available since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246807-plasmid-map-sequence-id-494296

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  14. TMPRSS2-Mutant-R255A

    Plasmid
    #240612
    Purpose
    To test the cleavage of ACE2 by a non-autocleavable form of TMPRSS2 (Less active form)
    Depositor
    Zaid Abassi
    Insert
    Type II transmembrane serine protease (TMPRSS2 Human)
    Expression
    Mammalian
    Mutation
    Arginine (R) at position 255 is replaced by alaniā€¦
    Promoter
    EF-1a
    Available since
    Aug. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    240612-plasmid-map-sequence-id-484527

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Showing: 9961 - 9980 of 12205 results