We narrowed to 19,287 results for: tes

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pLentiRNACRISPR_009-TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-EGFP

Plasmid

#223174

Purpose

Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

Depositor

Neville Sanjana

Publication

Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

Insert

RfxCas13d

Use

Lentiviral

Tags

NLS-HA

Available since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pMRX-IBU-pSu9-HaloTag7-SNAP

Plasmid

#217319

Purpose

Expresses the presequence of Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag for measuring mitophagy activity by Halo processing assay.

Depositor

Noboru Mizushima

Publication

Yang et al EMBO J. 2024 Nov;43(22):5613-5634. doi:

Insert

Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag

Use

Retroviral

Tags

HaloTag and SNAP

Promoter

CMV

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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p207-Switch-ON (FRT)

Plasmid

#217886

Purpose

Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

Depositor

Ulrich Elling

Publication

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

hU6

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pKUNOC-ErbB1-mScarlet

Plasmid

#197360

Purpose

Encoding human ErbB1 tagged with mScarlet.

Depositor

Michiyuki Matsuda

Publication

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ERBB1 (EGFR Human)

Tags

mScarlet

Expression

Mammalian

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/TO/V5-DEST-IGFBP2-Clover

Plasmid

#191008

Purpose

Lentiviral expression vector for IGFBP2 with a fluorescent Clover tag

Depositor

Johanna Ivaska

Publication

Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

Insert

Insulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)

Use

Lentiviral

Tags

Clover

Promoter

CMV

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcmv3-IRG1-H159Q-HA

Plasmid

#198183

Purpose

Mammalian expression of HA-tagged human ACOD1 (IRG1) H159Q

Depositor

Yun Zhao

Publication

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ACOD1 (ACOD1 Human)

Tags

HA

Expression

Mammalian

Mutation

The histidine in IRG1 at position 159 mutates to …

Promoter

CMV

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcmv3-IRG1-L272Q-HA

Plasmid

#198184

Purpose

Mammalian expression of HA-tagged human ACOD1 (IRG1) L272Q

Depositor

Yun Zhao

Publication

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ACOD1 (ACOD1 Human)

Tags

HA

Expression

Mammalian

Mutation

The leucine in IRG1 at position 272 mutates to gl…

Promoter

CMV

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-BMAL1-S513A/S515A/S516A

Plasmid

#186835

Purpose

Expresses FLAG-BMAL1-S513A/S515A/S516A in mammalian cells

Depositor

Hung-Chun Chang

Publication

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

bmal1 (Bmal1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 513/515/516 to Alanine

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG-HUWE1_R4187C

Plasmid

#187142

Purpose

mammalian cell expression of 3xFLAG-HUWE1 R4187C

Depositor

Eric Fischer

Publication

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HUWE1 (HUWE1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

R4187C

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSN838

Plasmid

#184826

Purpose

Expresses human KIF5A(∆exon27) in mammalian cells

Depositor

Kyoko Chiba

Publication

Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

Insert

KIF5A (∆exon27) (KIF5A Human)

Tags

mScarlet

Expression

Mammalian

Mutation

∆exon27 form

Promoter

CMV

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_CALM1_EF-hand

Plasmid

#178468

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Publication

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CALM1_EF-hand (CALM1 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Hygro DHFR.cHSF1

Plasmid

#134738

Purpose

Lentiviral expression vector for constitutively active HSF1 fused to DHFR destabilized domain

Depositor

Matthew D. Shoulders

Publication

Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

Insert

DHFR.cHSF1 (HSF1 Human)

Use

Lentiviral

Tags

DHFR

Expression

Mammalian

Mutation

Deletion of amino acids 186-202

Promoter

CMV

Available since

Oct. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mSNX27-ΔF3

Plasmid

#163618

Purpose

mouse SNX27 without the F3 domain cDNA C-terminally tagged with GFP

Depositor

Josef Kittler

Publication

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mouse SNX27-ΔF3 (Snx27 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

deletion F3 domain

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX330 NC-FKBP-Cas9

Plasmid

#162725

Purpose

Mammalian expression

Depositor

Amit Choudhary

Publication

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FKBP F36V

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pX330 NL-FKBP-Cas9

Plasmid

#162723

Purpose

Mammalian expression

Depositor

Amit Choudhary

Publication

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FKBP F36V

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRG706_lexO-SbfI_LexA-TF_LEU2MX

Plasmid

#154818

Purpose

Inducible expression of SbfI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Publication

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

SbfI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-Enhancer-ZC3H12D_PromoterWT

Plasmid

#153066

Purpose

ZC3H12D promoter region cloned upstream of luciferase reporter gene

Depositor

Silvia Monticelli

Publication

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ZC3H12D promoter region (ZC3H12D Human)

Use

Luciferase

Expression

Mammalian

Promoter

none

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-B41gp160

Plasmid

#123247

Purpose

Mammalian expression plasmid for Env from the B41 HIV-1 isolate

Depositor

Erik Procko

Publication

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (B41) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-CH111gp160

Plasmid

#123268

Purpose

Mammalian expression plasmid for Env from the CH111 HIV-1 isolate

Depositor

Erik Procko

Publication

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (AD8) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGEMT-Pax7bait-T2a-Cherry-pA

Plasmid

#111154

Purpose

knock in bait for Axolotl Pax7 locus

Depositor

Elly Tanaka

Publication

Fei et al Proc Natl Acad Sci U S A. 2017 Nov 21;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Pax7bait-T2a-Cherry-pA

Expression

Mammalian

Mutation

wt

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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