We narrowed to 713 results for: ELP

1. pET/ELP-I-CAT

   Plasmid

   #71461

   Purpose

   ELP-intein purification system

   Depositor

   David Wood

   Article

   Banki et al Nat Methods. 2005 Sep;2(9):659-61.

   Type

   Empty backbone

   Use

   Tags

   ELP ((VPGXG)110) and intein

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-A96 ELP

   Plasmid

   #67856

   Purpose

   GFP Fluorescent Elastin-like Polypeptide(VPGAG) with 96 ELP repeats containing Alanine guest residue

   Depositor

   Andrew MacKay

   Insert

   GFP fused to ELP (VPGAG) repeated 96 times
   

   Use

   Tags

   GFP fused to N-terminal of ELP

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-24a-ELP[V-30]

   Plasmid

   #67014

   Purpose

   Expresses ELP[V-30] in E. coli

   Depositor

   Ashutosh Chilkoti

   Article

   Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

   Insert

   ELP[V-30]
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET22b-TEVP-cleavableELP

   Plasmid

   #219402

   Purpose

   Tobacco etch virus protease-responsive elastin-like polypeptide (ELP)

   Depositor

   Danielle Mai

   Article

   Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

   Insert

   TEVP-cleavableELP
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SELP-bio-His

   Plasmid

   #51639

   Purpose

   Expresses full-length P-selectin ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   SELP (SELP Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMAL-c5X-ELP[AV-60]

   Plasmid

   #67012

   Purpose

   Expresses malE-ELP[AV-60] in E. coli

   Depositor

   Ashutosh Chilkoti

   Article

   Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

   Insert

   malE-ELP[AV-60]
   

   Use

   Tags

   His6 and malE-Factor Xa

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMAL-c5X-ELP[V-60]

   Plasmid

   #67013

   Purpose

   Expresses malE-ELP[V-60] in E. coli

   Depositor

   Ashutosh Chilkoti

   Article

   Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

   Insert

   malE-ELP[V-60]
   

   Use

   Tags

   His6 and malE-Factor Xa

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHelper-CBE-sgRNA

   Plasmid

   #221137

   Purpose

   Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

   Depositor

   Craig Roy

   Article

   Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

   Insert

   sgRNA-scaffold with Cas9 handle
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119(SpeI)

   Available since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-V96 ELP

   Plasmid

   #67853

   Purpose

   GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

   Depositor

   Andrew MacKay

   Article

   Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

   Insert

   GFP fused to ELP (VPGVG) repeated 96 times
   

   Use

   Tags

   GFP fused to N-terminal of ELP

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE80L TriCatcher-ELP

    Plasmid

    #112630

    Purpose

    Two SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcher

    Depositor

    Mark Howarth

    Article

    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

    Insert

    TriCatcher-ELP
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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