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Plasmid#175831PurposeExpress Sirius with plasma membrane-targeting sequence in mammalian cellsDepositorInsertSirius with plasma membrane-targeting sequence
UseTagsC-terminal hypervariable region of K-Ras 4BExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-SECFP-KRasCT
Plasmid#175832PurposeExpress SECFP with plasma membrane-targeting sequence in mammalian cellsDepositorInsertSECFP with plasma membrane-targeting sequence
UseTagsC-terminal hypervariable region of K-Ras 4BExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2 ER:ras
Plasmid#67844Purpose4-hydroxytamoxifen inducible ER:ras fusion proteinDepositorInsertER1a H-RasG12V fusion
UseTagsExpressionMammalianMutationG525R renders the HBD largely insensitive to 17ß‐…PromoterCMVAvailable sinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
2_T7-mScarlet3-KRas
Plasmid#225929PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras membrane localisation signalDepositorInsertmScarlet3-KRas
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
3_T7-mScarlet3-KRas6R
Plasmid#225930PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras6R membrane localisation signalDepositorInsertmScarlet3-KRas6R
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
human SLC12A5-V5-HA-2A-Luciferase repair template
Plasmid#158576PurposeFor targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.DepositorInserthuman SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
UseTagsV5 and HA on the endogenous KCC2 protein productExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R713-M88-303: CMV51p> Halotag-Hs.HRAS
Plasmid#159681PurposeMammalian protein expression of HRAS-Halotag fusionDepositorInsertHalotag-Hs.HRAS (HRAS Human)
UseTagsHalotagExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R713-M91-303: CMV51p> Halotag-Hs.NRAS
Plasmid#159682PurposeMammalian protein expression of NRAS-Halotag fusionDepositorInsertHalotag-Hs.NRAS (NRAS Human)
UseTagsHalotagExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
R713-M50-303: CMV51p> Halotag-HRasHVR
Plasmid#159685PurposeMammalian protein expression of HRAS HVR (Hypervariable region)-Halotag fusionDepositorInsertHalotag-HRasHVR (HRAS Human)
UseTagsHalotagExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
R713-M52-303: CMV51p> Halotag-NRasHVR
Plasmid#159686PurposeMammalian protein expression of NRAS HVR (Hypervariable region)-Halotag fusionDepositorInsertHalotag-NRasHVR (NRAS Human)
UseTagsHalotagExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRT006.3 L1 dual-luciferase AI retrotransposition reporter
Plasmid#213031PurposeBi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integrationDepositorInsertTet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
UseLuciferaseTagsFirefly luciferase antisense intron (3' UTR)ExpressionMammalianMutationPromoterpTRE bidirectional tet-onAvailable sinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-K-CAAX
Plasmid#41644PurposeExpresses wild-type Dronpa fused to the membrane-anchoring K-Ras C-terminal farnesylation motif (CAAX box)DepositorInsertDronpa145K-CAAX
UseTagsCAAX boxExpressionMammalianMutationPromoterCMVAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-Lg-K
Plasmid#216974PurposeExpression of Large BiT-Linker-GvpKDepositorInsertLarge BiT-Linker-GvpK
UseTagsFLAG-Split Nanoluciferase LargeBiTExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-K-Sm
Plasmid#216996PurposeExpression of GvpK-Linker-Small BiTDepositorInsertGvpK-Linker-Small BiT
UseTagsSplit Nanoluciferase SmallBiTExpressionBacterialMutationPromoterAvailable sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-Sm-K
Plasmid#216997PurposeExpression of Small BiT-Linker-GvpKDepositorInsertSmall BiT-Linker-GvpK
UseTagsSplit Nanoluciferase SmallBiTExpressionBacterialMutationPromoterAvailable sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-K-Lg
Plasmid#216973PurposeExpression of GvpK-Linker-Large BiTDepositorInsertGvpK-Linker-Large BiT
UseTagsSplit Nanoluciferase LargeBiT-FLAGExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-MMP9 matrix metalloproteinase-9 precursor [L51/82R]
Plasmid#149455PurposeMammalian Expression Plasmid of anti-MMP9 matrix metalloproteinase-9 precursor (Rat). Derived from hybridoma L51/82.DepositorInsertanti-MMP9 matrix metalloproteinase-9 precursor (Rattus norvegicus) recombinant mouse monoclonal antibody (Mmp9 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Anti-Copper ATPase 1 (Menke's disease protein) [L60/4R]
Plasmid#114502PurposeMammalian Expression Plasmid of anti-Copper ATPase 1 (Menke's disease protein) (Human). Derived from hybridoma L60/4.DepositorInsertanti-Copper ATPase 1 (Menke's disease protein) (Homo sapiens) recombinant mouse monoclonal antibody (ATP7A Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
K-to-R mutant NPRL2(1-380) in pLJM60
Plasmid#184562PurposeCMV-driven expression of an untagged mutant NPRL2 (core subunit of GATOR1) — native sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.DepositorInsertNPRL2 (NPRL2 Human)
UseLentiviralTagsExpressionMammalianMutationAll lysines were mutated to arginines.PromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPLP1.3
Plasmid#22615DepositorInsertProteolipid Protein
UseE. coli phagemid vectorTagsExpressionMutationPromoterAvailable sinceNov. 24, 2009AvailabilityAcademic Institutions and Nonprofits only