We narrowed to 675 results for: intein

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pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pA

Plasmid

#140223

Purpose

Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activator

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein

Expression

Mammalian

Mutation

D832A

Promoter

PGK

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-408 FLAG UnaG[1] Stuffer/InteinD UnaG[2]

Plasmid

#130156

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

UnaG

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-397 FLAG Venus210[1] Stuffer/InteinA Venus210[2]

Plasmid

#130145

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Venus

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTYB3-human PUM1-HD MUT7-1 (E1083Q)-intein-CBD

Plasmid

#73303

Purpose

Expresses mutant human PUM1-HD

Depositor

Traci Hall

Article

Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

Insert

human PUM1 Pumilio homology domain (PUM1 Human)

Tags

intein

Expression

Bacterial

Mutation

E1083Q

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-nTermPuroR-nTermIntein

Plasmid

#208378

Purpose

Episomal plasmid - for expression of 2 proteins linked with EGFP and n-terminal split puromycin resistance and split intein

Depositor

Daniel Hawiger

Article

Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

Type

Empty backbone

Expression

Mammalian

Promoter

human CMV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[21-881]-C-int-G418R

Plasmid

#199198

Purpose

Insert to create split-intein Gal4[21-881]-C-int knock-in vectors, with G418 resistance, via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[21-881]-C-int-G418R

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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ISG15 C-term. Domain intein/chitin binding domain (79-156)

Plasmid

#110760

Purpose

Bacterial expression of ISG15 C-term.

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Tags

intein/chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTYB3-human PUM1-HD MUT3-2 (C935S-Q939E)-intein-CBD

Plasmid

#73302

Purpose

Expresses mutant human PUM1-HD

Depositor

Traci Hall

Article

Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

Insert

human PUM1 Pumilio homology domain (PUM1 Human)

Tags

intein

Expression

Bacterial

Mutation

C935S-Q939E

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH

Plasmid

#112211

Purpose

Targeted DNA methylation

Depositor

Xingxu Huang

Article

Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

Insert

C-terminus of dCas9

Use

AAV

Mutation

D10A

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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AAV Intein-C-SpyCas9, CMV-AcrIIA4-scaffold (2xBsmBI sites)

Plasmid

#120294

Purpose

AAV Vector for expression of C-terminal SpyCas9 fragemnt with split-intein and a CMV-driven AcrIIA4 (no miR binding sites)

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

C-terminal fragment of SpyCas9

Use

AAV and CRISPR

Tags

split-intein

Expression

Mammalian

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-inteinC-Sauri C aa439-1061-U6-Camk2d sgRNA7

Plasmid

#220129

Purpose

Expresses Sauri cas9C by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, inteinC, nSauri Cas9C

Use

AAV and CRISPR

Expression

Mammalian

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-inteinC-SpC aa714-1368-U6-Camk2d sgRNA7

Plasmid

#226916

Purpose

Expresses Sp cas9C by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, inteinC, nSp Cas9C

Use

AAV

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TNT4-TadA8e-SpRY N aa2-713-InteinN-miR122 TS

Plasmid

#209787

Purpose

Expresses TadA8e and SpRY cas9N by the specific TNT4 promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

TNT4, TadA8e, SpRY N, inteinN, miR122 TS

Use

AAV

Promoter

TNT4

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CASI-InteinC-SpRY C aa714-1368-U6-Camk2d sgRNA

Plasmid

#209788

Purpose

Expresses SpRY cas9C by the constitutive CASI promoter and sgRNA targeting the oxidative activation sites of Camk2d by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

SpRY C, Camk2d sgRNA

Use

AAV

Promoter

CASI

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CASI-InteinC-SpG C aa714-1368-U6-Lmna sgRNA1

Plasmid

#208110

Purpose

Expresses SpG cas9C by the constitutive CASI promoter and sgRNA targeting murine Lmna c.1621T mutation by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

Insert

SpG C, U6, Lmna sgRNA1, scaffold

Use

AAV

Promoter

U6

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTYB3-human PUM1-HD MUT7-2 (S1079N-E1083Q)-intein-CBD

Plasmid

#73299

Purpose

Expresses mutant human PUM1-HD

Depositor

Traci Hall

Article

Cheong et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

Insert

human PUM1 Pumilio homology domain (PUM1 Human)

Tags

intein

Expression

Bacterial

Mutation

S1079N-E1083Q

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
ISG15 C-term. Domain deltaC intein/chitin binding domain (79-154)

Plasmid

#110761

Purpose

Bacterial expression of proISG15

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Tags

intein/chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-TadA8e-SpN aa2-713- inteinN-U6-Camk2d sgRNA7

Plasmid

#226915

Purpose

Expresses TadA8e and Sp cas9N by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSp Cas9N, inteinN

Use

AAV

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-HA-TadA8e-Sauri N aa1-438-inteinN-U6-Camk2d sgRNA7

Plasmid

#226678

Purpose

Expresses TadA8e and Sauri cas9N by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSauri Cas9N, inteinN

Use

AAV

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pRDAX-491 GFP[1] L3 Stuffer/Intein3 L4 GFP[2] IRES mCherry GFP(1-9)

Plasmid

#130174

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

GFP

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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