Addgene

We narrowed to 2,134 results for: mt

Showing: 1471 - 1500 of 2134 results

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pST_AdAPOA4-44 (pBS0745)

Plasmid

#185236

Purpose

For the mammalian expression of the Chinese giant salamander protein AdAPOA4-44. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AdAPOA4-44

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOA4_ANDDA_52-358 (pBS0744)

Plasmid

#185235

Purpose

For the mammalian expression of the Chinese giant salamander protein APOA4_ANDDA_52-358. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOA4_ANDDA_52-358

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pL-mWasabi

Plasmid

#176552

Purpose

Expression of mWasabi for auxotrophic selection in the absence of leucine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

mWasabi

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH1(GAP)

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pM-eCFP

Plasmid

#176558

Purpose

Expression of eCFP for auxotrophic selection in the absence of methionine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

eCFP

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH1(GAP)

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pH-TagRFP657

Plasmid

#176560

Purpose

Expression of TagRFP657 for auxotrophic selection in the absence of lysine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

TagRFP657

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH1(GAP)

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pM-TagRFP657

Plasmid

#176563

Purpose

Expression of TagRFP657 for auxotrophic selection in the absence of methionine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

TagRFP657

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH1(GAP)

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV ShadowY-AURKA-mTurq2

Plasmid

#157770

Purpose

Expression of AuroraA kinase biosensor under CMV promoter

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Use

Tags

mTurquoise2 and shadowY

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP N1 AURKA deltaNter

Plasmid

#157753

Purpose

Expression of AURKA without Nter, fused to GFP in mammalian cells

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 AURKA deltaNter

Plasmid

#157749

Purpose

Expression of AURKA without Nter in mammalian cells

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Use

Tags

Expression

Mammalian

Mutation

the first 30AA of Aurora A are lacking

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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FMR1-E17B-P2A-NLuc

Plasmid

#157857

Purpose

donor plasmid for FMR1-Nluc

Depositor

Xinyu Zhao

Article

Li et al Stem Cells. 2017 Jan;35(1):158-169. doi:

Insert

FMR1-Nlu (FMR1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pHA853 - mec-4p::Fynempty

Plasmid

#139210

Purpose

Control vector for Fyn kinase expression in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Use

Tags

Expression

Worm

Mutation

deletion and frame shift to prevent Fyn expression

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA851 - osm-10p::Fynempty

Plasmid

#139208

Purpose

Control vector for Fyn kinase expression in osm-10 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Use

Tags

Expression

Worm

Mutation

deletion and frame shift to prevent Fyn expression

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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FUS_LC_charge_RtoK

Plasmid

#139127

Purpose

express FUS LC with hnRNPA2-like charge patterning where all R are changed to K

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

FUS (FUS Human)

Use

Tags

Expression

Bacterial

Mutation

Add K and D to FUS LC so it has the same charge p…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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FUS_LC_charge

Plasmid

#139126

Purpose

express FUS LC with an hnRNPA2 LC-like charge patterning

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

FUS (FUS Human)

Use

Tags

Expression

Bacterial

Mutation

Add R, K, D to make FUS LC have similar charge pa…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-NCOA7g3 (BB24)

Plasmid

#139459

Purpose

Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a / U6

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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S2 1058 AIDx no UGI

Plasmid

#135349

Purpose

Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx-ID-1058

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S2 1058 APOBEC3 no UGI

Plasmid

#135350

Purpose

Expression of intradomain insertion of rat cytosine deaminase, rAPOBEC3, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-rAPOBEC3-ID-1058

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
S1 1029 CP APOBEC3 no UGI

Plasmid

#135352

Purpose

Expression of circular permutant of nSpyCas9 lacking uracil DNA glycosylase inhibitor at amino acid position 1029 encoding rat cytosine deaminase, rAPOBEC3 at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

rAPOBEC3-nSpyCas9 aa 1029

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S1 1029 AIDx with UGI

Plasmid

#135354

Purpose

Expression of nSpyCas9 encoding truncated human cytosine deaminase hAIDx at amino acid 1029 and uracil DNA glycosylase inhibitor (UGI) at the C terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

hAIDx-nSpyCas9 aa-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S1 1029 CP ABEMax strategy 2

Plasmid

#135358

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding de-coupled ABEMax (wildtype Tada monomer at N terminus, evolved Tada monomer at C terminus)

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

WT Tada-nSpyCas9 aa 1029-evolved Tada

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
S1 1029 CP ABEMax strategy 3

Plasmid

#135359

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the C-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

1029 CP nSpyCas9 ABEMax

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S1 1029 miniABEMax V82G strategy1

Plasmid

#135360

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

mini ABEMax 1029 nSpyCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
S1 1029 V82G ABEMax Strategy 3

Plasmid

#135363

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

1029 V82G ABEMax

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1816

Plasmid

#154320

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet-I^SEC (Lox511I)^3xMyc

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1827

Plasmid

#154323

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet-I

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1841

Plasmid

#154338

Purpose

Promoter reporter

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR

Use

Tags

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1948

Plasmid

#154340

Purpose

Enhancer reporter

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

pes-10delta::SV40 NLS::mScarlet_I::PEST(dpi)::-tbb-2 3'UTR

Use

Tags

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT

Plasmid

#61789

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad2 with MRE-2 and MRE-3 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad2 3'UTR with both miR-200c MRE-2 and MRE-3 mutated

Use

Luciferase

Tags

Expression

Mutation

both miR-200c MRE-2 and MRE-3 mutated

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pGEX-2TK-WW-CR-W3083F/P3086A

Plasmid

#18077

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Second conserved Tryptophan (W) of the WW domain …

Promoter

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-2TK-WW-CR-d-ZZ-W3083F

Plasmid

#18078

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Second conserved Tryptophan (W) of the WW domain …

Promoter

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only