Addgene

We narrowed to 3,140 results for: cat.3

Showing: 981 - 1000 of 3140 results

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49535-sgFMR1_E17A

Plasmid

#157782

Purpose

Expresses a sgRNA targeting stop codon of human FMR1 gene

Depositor

Xinyu Zhao

Article

Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

Insert

FMR1 (FMR1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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ZsGreen1-cMYC/pLVX-Puromycin

Plasmid

#180278

Purpose

Plasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).

Depositor

Andre Catic

Article

Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

Insert

ZsGreen1-cMYC fusion protein MYC (MYC Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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Epac-S-H90

Plasmid

#170340

Purpose

CFP(UST)_EPACdDEPCD_cp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

Depositor

Kees Jalink

Article

van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

Insert

CFP(UST)_EPACdDEPCD_cp173Venus(ST) (RAPGEF3 Human)

Use

Tags

Expression

Mammalian

Mutation

Deletion of DEP domain, Catalytically dead T781A …

Promoter

CMV

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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Epac-S-H81

Plasmid

#170339

Purpose

GFPDel(UST)_EPACdDEPCD_mRFP biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

Depositor

Kees Jalink

Article

van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

Insert

GFPDel(UST)_EPACdDEPCD_mRFP (RAPGEF3 Human)

Use

Tags

Expression

Mammalian

Mutation

Deletion of DEP domain, Catalytically dead T781A …

Promoter

CMV

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_F753A_6xH

Plasmid

#185710

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed F753 to A

Promoter

T7

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pUC57-HBB-nLuc

Plasmid

#175432

Purpose

Used to generate IVT RNA for translation extracts

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

HBB-nLuc

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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AAV8-cPE-C

Plasmid

#183539

Purpose

Deliver a split compact Prime Editor in vivo

Depositor

Wen Xue

Article

Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

Insert

C-terminal fragment of SpCas9 nickase (H840A), Reverse Transcriptase

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U1A

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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AAV8-cPE-N

Plasmid

#183538

Purpose

Deliver a split compact Prime Editor in vivo

Depositor

Wen Xue

Article

Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

Insert

N-terminal fragment of SpCas9 nickase (H840A)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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3xFLAG-SENP6

Plasmid

#42887

Depositor

Edward Yeh

Article

Dou et al Mol Cell. 2010 Aug 13. 39(3):333-45.

Insert

SENP6 (SENP6 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Please see depositor comments

Promoter

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP_mEGFP-GluA1c

Plasmid

#114136

Purpose

Mammalian expression of the cytosolic C terminus of GluA1 N-terminally tagged by mEGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

mEGFP tagged GluA1 C-terminus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_W403A_F753A_6xH

Plasmid

#185713

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed W403 and F753 to A

Promoter

T7

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pQE60 R130A wgMDH

Plasmid

#204266

Purpose

Bacterial expression of R130A wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

R130A

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Level 1 P1 OsActinP:Hygint:NosT

Plasmid

#165423

Purpose

GoldenGate (MoClo) Level 1 Position 1 (Reverse) hygromycin selection cassette

Depositor

John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

Article

Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

Insert

Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator

Use

Synthetic Biology; L1 p1(r) golden gate (moclo) h…

Tags

Expression

Mutation

Promoter

Os Actin promoter :: Hygromycin resistance gene (…

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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GST-p25

Plasmid

#24896

Depositor

Harish Pant

Article

Amin et al J Neurosci Res. 2002 Feb 1. 67(3):354-62

Insert

p25 (CDK5R1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

N-terminal truncated version of p35, contains aa …

Promoter

Available since

May 17, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3 Flag TSC2 (S939A/T1462A)

Plasmid

#14131

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 S939A/T1462A (TSC2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S939A/T1462A

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
C3 luc (T1 del)

Plasmid

#11358

Depositor

Donald McDonnell

Article

Fan et al Mol Endocrinol. 1996 Dec . 10(12):1605-1

Insert

C3 promoter (C3 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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pQE60 R124K wgMDH

Plasmid

#204258

Purpose

Bacterial expression of R124K wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

R124K

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 R124Q wgMDH

Plasmid

#204259

Purpose

Bacterial expression of R124Q wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

R124Q

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 R124A wgMDH

Plasmid

#204257

Purpose

Bacterial expression of R124A wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

R124A

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 R130E wgMDH

Plasmid

#204267

Purpose

Bacterial expression of R130E wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

R130E

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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