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pSIREN-RetroQ-MYB-shRNA

Plasmid

#25790

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

shRNA against human MYB (MYB Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
PTPRA_deltaD1-SHC

Plasmid

#139615

Purpose

StrepIII-HA-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

PTPRA_deltaD1 (PTPRA Human)

Tags

StrepIII-HA

Expression

Mammalian

Mutation

D1 domain deletion

Promoter

CMV

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV iGluu

Plasmid

#105832

Purpose

Fast indicator for extracellular glutamate imaging

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR S72T variant

Tags

Myc and PDGFR

Expression

Mammalian

Mutation

Changed Ser 72 to Thr

Promoter

CMV

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pRSV-CaMKIV

Plasmid

#45062

Purpose

Expression of CaMKIV

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

Cam Kinase IV (Camk4 Mouse)

Expression

Mammalian

Mutation

*

Promoter

RSV

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pCR4-huNFATc1nuc

Plasmid

#23988

Depositor

Jerry Crabtree

Article

Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

Insert

NFATc1 (NFATC1 Human)

Use

Cloning vector

Tags

EE tag

Mutation

S172A S175A S176A S178A S179A S181A S184A S187A S…

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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pGEX-p53 R175H

Plasmid

#39480

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Insert

TP53 (TP53 Human)

Tags

GST

Expression

Bacterial

Mutation

P72R and R175H

Available Since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only
pSP64T-hLbCpf1

Plasmid

#107187

Purpose

plasmid to carry out IVT of human codon optimized LbCpf1 (based on Addgene 69988)

Depositor

Antonio Giraldez

Article

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

hLbCpf1

Use

CRISPR

Tags

NLS-3xHA

Promoter

sp6

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMIR-Dsred-IRES-His-Halo-Tev-Keap C288S

Plasmid

#62459

Purpose

expresses his-tagged C288S human Keap1 protein

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Mammalian

Mutation

Changed Cysteine 288 of Keap1 to serine

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLHCX-Flag-mPKM2(C358S)

Plasmid

#42513

Depositor

Dimitrios Anastasiou

Article

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

Pyruvate Kinase M2 Cys358->Ser

Use

Retroviral

Tags

Flag

Mutation

Changed Cysteine 358 to Serine

Promoter

CMV

Available Since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-UBE2QL1-Strep/HA

Plasmid

#124666

Purpose

Expresses UBE2QL1 with C-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 (UBE2QL1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC-NMYC-mTRF1

Plasmid

#64162

Purpose

Retroviral vector expressing mouse TRF1 with N-terminal Myc tag

Depositor

Titia de Lange

Article

Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

Insert

mTRF1 (Terf1 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-Flag-H2A K5-9R

Plasmid

#63562

Purpose

Expression of human H2A with K5R and K9R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine 5 and Lysine 9 are now Arginine

Promoter

CMV

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only
LPCX BMP-RIB Q203D

Plasmid

#12642

Depositor

Rik Derynck

Article

Skillington et al J Cell Biol. 2002 Oct 14. 159(1):135-46.

Insert

BMP-RIB (Bmpr1b Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Q203D, activating mutation

Available Since

Oct. 17, 2006

Availability

Academic Institutions and Nonprofits only
AA008

Plasmid

#216003

Purpose

Fragmid fragment: (Cas protein) preferred over wildtype Cas12a

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas12a_v1.1 [EnAs]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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dCas9-CRY2Clust-FUSN-mut

Plasmid

#200970

Purpose

induction of phase-separated condensate mediated by FUSN-IDR mutant

Depositor

Jian Xu

Article

Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

Insert

FUSNmut

Use

Lentiviral

Expression

Bacterial and Mammalian

Mutation

15 Tyr-to-Ser substitutions

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pT3TS-zAsCpf1

Plasmid

#107185

Purpose

plasmid to carry out IVT of AsCpf1 (zebrafish codon optimized)

Depositor

Antonio Giraldez

Article

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

AsCpf1

Use

CRISPR

Tags

NLS-3xHA

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pL6-H2B-iRFP720-puro

Plasmid

#189992

Purpose

Lentivirial plasmid for nuclear iRFP-720 expression with puromycin resistance

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Human histone H2B

Use

Lentiviral

Tags

iRFP720

Expression

Mammalian

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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Sa-dCas9-NLS-3xFLAG/pcDNA3.1

Plasmid

#98041

Purpose

Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

Sa-dCas9-NLS-3xFLAG

Use

CRISPR

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

D10A + H557A

Promoter

CMV

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X1 Flag hDPY30

Plasmid

#15556

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

DPY30 (DPY30 Human)

Tags

Flag and GST

Expression

Bacterial

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pLX302-JSP1-V5 puro

Plasmid

#87773

Purpose

Expresses V5 tagged human JSP1 (DUSP22)

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

JSP1 (DUSP22 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

March 23, 2017

Availability

Academic Institutions and Nonprofits only

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