We narrowed to 25,364 results for: ina
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Plasmid#197114PurposeExpression of kinase-deficient GFP-tagged TAOK2 (K57A) / PSK1 (K57A) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTAOK2 (K57A) (TAOK2 Human)
TagsGFPExpressionMammalianMutationChanged K 57 to APromoterCMVAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-mCherry-Rheb(C181S)
Plasmid#192438PurposeEncodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLyso-mCherry-Rheb(C181S) (RHEB Human)
TagsFull-length LAMP1 and mCherryExpressionMammalianMutationRheb Cys 181 mutated to Ser; abolishes C-terminal…PromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-Rheb(C181S)
Plasmid#192437PurposeEncodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry-Rheb(C181S) (RHEB Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationRheb Cys 181 mutated to Ser; abolishes C-terminal…PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
mcherry-p62 delta PB1 silent
Plasmid#187985Purposehuman p62 lacking PB1 domain (=aa1-102) with a silent mutation to get resistance for sip62 #5 (Dharmacon). Internal Reference: SMC552DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertp62 (NUP62 Human)
TagsmCherryExpressionMammalianMutationLacking PB1 domain (aa1-102) with a silent mutati…Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDNA3-Cerulean-FHA1-NES
Plasmid#138202PurposeEncodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCerulean-FHA1-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mVenus-NES-SspB-SOS2cat-P2A-iLIDcaax
Plasmid#173867PurposeMammalian expression of mVenus-NES-SspB-SOS2cat-P2A-iLIDcaax (opto-Ras)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmVenus-NES-SspB-SOS2cat-P2A-iLIDcaax
TagsC-terminal CAAX-box and mVenusExpressionMammalianPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 hMOG-alpha2-EGFP
Plasmid#160976PurposeExpresses a human myelin oligodendrocyte glycoprotein isoform alpha2 - EGFP fusion protein in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHomo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant alpha2 (MOG Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 hMOG-alpha3-EGFP
Plasmid#160977PurposeExpresses a human myelin oligodendrocyte glycoprotein isoform alpha3 - EGFP fusion protein in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHomo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant alpha3 (MOG Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IPU-FLAG-NEK9T210A
Plasmid#168270PurposeExpresses kinase-dead NEK9 mutant in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNEK9 (NEK9 Human)
TagsFLAGExpressionMammalianMutationchanged threonine 210 to alanineAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)
Plasmid#169168PurposeConjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.DepositorInsertMAP1LC3B (MAP1LC3B Human)
Tags6X Histidine Tag, TEV cleavage site, mCherryExpressionBacterialPromoterT7 lac promoterAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGBW-m4092686
Plasmid#153895PurposeBacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTRDepositorInsertfragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionBacterialMutationNearly native encoding;inactivating mutations in …PromoterPromoter | t7 consensus from NEB, G is +1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBa.GFP-KIF1Balpha tail 387-1150
Plasmid#134619PurposeMammalian expression of mouse KIF1Balpha 387-1150DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKIF1Balpha (Kif1b Mouse)
TagseGFPExpressionMammalianMutationWT with deletion of the 386 N-terminal residues.PromoterChicken Beta actinAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_WW-PolyA
Plasmid#112290PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) WW mutant. WQDP (199-202) in the first WW domain was changed to AQDA, and the WLDP (258-261) of the second WW domain was changed to ALDADepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutationHuman YAP1-2gamma with mutations in the WW domain…PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231G
Plasmid#113953Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationdelete N-terminal 21 residues, I132S, S212N, and …PromotercmvAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKAR1-NES
Plasmid#84632PurposeUnimolecular, FRET-based JNK activity reporter; cytosol targetedDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertECFP-FHA1-JNKsubDD-Citrine-NES
Tags6xHis, Citrine, ECFP, Nuclear export signal (NES)…ExpressionMammalianPromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-ATL1-G20TM
Plasmid#109019Purposestable lentiviral expression of cDNADepositorInsertATL1-deltaTM (ATL1 Human)
UseLentiviralTagseGFPExpressionMammalianMutationtruncated form without C-terminal ER transmembran…PromoterEIF-1a promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
B270 + SMARCAL1 sgSTOP
Plasmid#100717PurposeB270 plasmid expressing SMARCAL1 sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgSTOP targeting SMARCAL1 (cloned using BbsI) and ATP1A1 (SMARCAL1 Human)
UseCRISPRExpressionMammalianPromoterU6 promotersAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP3-44
Plasmid#91780PurposeMammalian expression of low affinity calcium-sensing fluorescent proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGCaMP3-44
Tags6xHis, T7 epitope, and Xpress tagExpressionMammalianPromoterCMVAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTE4566
Plasmid#88904PurposeExpresses MbCpf1 crRNA and inactive/dead, humanized MbCpf1 nucleaseDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsMb crRNA
hMbCpf1(D986A)
UseCRISPRTags3xHA and NLSExpressionMammalianPromoterCMV and human U6Available sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 8 GFP RFP
Plasmid#83564PurposeExpresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.DepositorInsertPKC alpha (PRKCA Human)
Tags10 nm ER/K Helix, FLAG purification tag, TEV Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only